Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF88 and GFF89 are separated by 194 nucleotides GFF89 and GFF90 overlap by 4 nucleotides GFF90 and GFF91 are separated by 120 nucleotides
GFF88 - glutamyl-Q-tRNA synthetase, at 72,716 to 73,657
GFF88
GFF89 - Poly(A) polymerase (EC 2.7.7.19), at 73,852 to 75,216
GFF89
GFF90 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3), at 75,213 to 75,692
GFF90
GFF91 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), at 75,813 to 76,604
GFF91
Position (kb) 73
74
75
76 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 72.852 kb on + strand, within GFF88 at 72.853 kb on - strand, within GFF88 at 72.853 kb on - strand, within GFF88 at 72.988 kb on - strand, within GFF88 at 72.988 kb on - strand, within GFF88 at 73.059 kb on - strand, within GFF88 at 73.059 kb on - strand, within GFF88 at 73.059 kb on - strand, within GFF88 at 73.160 kb on - strand, within GFF88 at 73.276 kb on + strand, within GFF88 at 73.373 kb on + strand, within GFF88 at 73.374 kb on - strand, within GFF88 at 73.375 kb on + strand, within GFF88 at 73.626 kb on + strand at 73.720 kb on - strand at 73.720 kb on - strand at 73.792 kb on + strand at 73.797 kb on + strand at 74.326 kb on + strand, within GFF89 at 74.605 kb on + strand, within GFF89 at 74.655 kb on + strand, within GFF89 at 74.842 kb on + strand, within GFF89 at 74.916 kb on + strand, within GFF89 at 74.934 kb on + strand, within GFF89 at 75.636 kb on - strand, within GFF90 at 75.773 kb on - strand at 75.773 kb on - strand at 75.796 kb on + strand at 75.907 kb on + strand, within GFF91 at 75.923 kb on + strand, within GFF91 at 75.924 kb on - strand, within GFF91 at 76.005 kb on - strand, within GFF91 at 76.051 kb on + strand, within GFF91 at 76.074 kb on - strand, within GFF91
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 72,852 + GFF88 0.14 -0.9 72,853 - GFF88 0.15 +0.4 72,853 - GFF88 0.15 +0.3 72,988 - GFF88 0.29 +0.1 72,988 - GFF88 0.29 +2.6 73,059 - GFF88 0.36 -0.8 73,059 - GFF88 0.36 +0.3 73,059 - GFF88 0.36 -1.4 73,160 - GFF88 0.47 -0.9 73,276 + GFF88 0.59 -0.2 73,373 + GFF88 0.70 +0.0 73,374 - GFF88 0.70 -0.4 73,375 + GFF88 0.70 +0.5 73,626 + -1.2 73,720 - -2.3 73,720 - -5.1 73,792 + +1.1 73,797 + +0.5 74,326 + GFF89 0.35 -1.2 74,605 + GFF89 0.55 -2.8 74,655 + GFF89 0.59 -2.5 74,842 + GFF89 0.73 -1.1 74,916 + GFF89 0.78 -2.3 74,934 + GFF89 0.79 -0.4 75,636 - GFF90 0.88 -2.0 75,773 - +1.2 75,773 - +0.4 75,796 + +0.4 75,907 + GFF91 0.12 -0.6 75,923 + GFF91 0.14 +0.7 75,924 - GFF91 0.14 -0.5 76,005 - GFF91 0.24 +0.5 76,051 + GFF91 0.30 +0.2 76,074 - GFF91 0.33 -0.2
Or see this region's nucleotide sequence