Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF567 and GFF568 are separated by 468 nucleotides GFF568 and GFF569 are separated by 123 nucleotides GFF569 and GFF570 are separated by 171 nucleotides
GFF567 - Putative acetyltransferase, at 296,973 to 297,461
GFF567
GFF568 - FIG00553873: hypothetical protein, at 297,930 to 298,967
GFF568
GFF569 - Qercetin 2,3-dioxygenase, at 299,091 to 299,786
GFF569
GFF570 - Gluconate utilization system Gnt-I transcriptional repressor, at 299,958 to 300,911
GFF570
Position (kb) 297
298
299 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 297.069 kb on - strand, within GFF567 at 297.071 kb on - strand, within GFF567 at 297.116 kb on + strand, within GFF567 at 297.261 kb on - strand, within GFF567 at 297.308 kb on + strand, within GFF567 at 297.370 kb on - strand, within GFF567 at 297.383 kb on - strand, within GFF567 at 297.467 kb on - strand at 297.490 kb on - strand at 297.699 kb on + strand at 297.741 kb on + strand at 297.741 kb on + strand at 297.747 kb on + strand at 297.748 kb on - strand at 297.895 kb on - strand at 297.895 kb on - strand at 297.937 kb on + strand at 297.968 kb on + strand at 298.011 kb on - strand at 298.145 kb on + strand, within GFF568 at 298.146 kb on - strand, within GFF568 at 298.176 kb on - strand, within GFF568 at 298.467 kb on - strand, within GFF568 at 298.478 kb on + strand, within GFF568 at 298.513 kb on - strand, within GFF568 at 298.532 kb on + strand, within GFF568 at 298.712 kb on + strand, within GFF568 at 298.712 kb on + strand, within GFF568 at 298.784 kb on + strand, within GFF568 at 298.898 kb on + strand at 299.030 kb on + strand at 299.059 kb on + strand at 299.097 kb on + strand at 299.289 kb on + strand, within GFF569 at 299.289 kb on + strand, within GFF569 at 299.289 kb on + strand, within GFF569 at 299.289 kb on + strand, within GFF569 at 299.290 kb on - strand, within GFF569 at 299.290 kb on - strand, within GFF569 at 299.290 kb on - strand, within GFF569 at 299.294 kb on - strand, within GFF569 at 299.653 kb on + strand, within GFF569 at 299.654 kb on - strand, within GFF569 at 299.654 kb on - strand, within GFF569 at 299.804 kb on + strand at 299.892 kb on - strand at 299.901 kb on - strand at 299.901 kb on - strand at 299.903 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 297,069 - GFF567 0.20 +0.4 297,071 - GFF567 0.20 -0.7 297,116 + GFF567 0.29 +3.1 297,261 - GFF567 0.59 +0.2 297,308 + GFF567 0.69 -1.1 297,370 - GFF567 0.81 -0.1 297,383 - GFF567 0.84 +0.6 297,467 - -0.3 297,490 - +0.1 297,699 + +0.2 297,741 + +0.2 297,741 + -0.8 297,747 + -0.6 297,748 - +0.1 297,895 - +0.2 297,895 - +0.3 297,937 + +0.5 297,968 + +0.4 298,011 - -0.8 298,145 + GFF568 0.21 -0.2 298,146 - GFF568 0.21 +0.7 298,176 - GFF568 0.24 -0.8 298,467 - GFF568 0.52 +1.6 298,478 + GFF568 0.53 -0.3 298,513 - GFF568 0.56 +4.4 298,532 + GFF568 0.58 +1.7 298,712 + GFF568 0.75 -0.1 298,712 + GFF568 0.75 -0.4 298,784 + GFF568 0.82 -1.5 298,898 + +0.5 299,030 + +0.6 299,059 + -0.7 299,097 + +0.0 299,289 + GFF569 0.28 +1.3 299,289 + GFF569 0.28 +0.6 299,289 + GFF569 0.28 -1.0 299,289 + GFF569 0.28 -0.6 299,290 - GFF569 0.29 +0.7 299,290 - GFF569 0.29 -0.8 299,290 - GFF569 0.29 -0.4 299,294 - GFF569 0.29 +3.5 299,653 + GFF569 0.81 +0.4 299,654 - GFF569 0.81 +0.2 299,654 - GFF569 0.81 -0.1 299,804 + -0.2 299,892 - -0.4 299,901 - +0.0 299,901 - -1.0 299,903 - -0.3
Or see this region's nucleotide sequence