Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF552 and GFF553 are separated by 131 nucleotides GFF553 and GFF554 are separated by 107 nucleotides GFF554 and GFF555 are separated by 258 nucleotides
GFF552 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 283,711 to 284,424
GFF552
GFF553 - RelB/StbD replicon stabilization protein (antitoxin to RelE/StbE), at 284,556 to 284,777
GFF553
GFF554 - Death on curing protein, Doc toxin, at 284,885 to 285,142
GFF554
GFF555 - Glycerol-3-phosphate ABC transporter, periplasmic glycerol-3-phosphate-binding protein (TC 3.A.1.1.3), at 285,401 to 286,717
GFF555
Position (kb) 284
285
286 Strain fitness (log2 ratio) -1
0
1
2
3 at 283.908 kb on + strand, within GFF552 at 283.917 kb on - strand, within GFF552 at 284.059 kb on - strand, within GFF552 at 284.072 kb on - strand, within GFF552 at 284.183 kb on + strand, within GFF552 at 284.184 kb on - strand, within GFF552 at 284.410 kb on + strand at 284.411 kb on - strand at 284.530 kb on - strand at 284.538 kb on - strand at 284.571 kb on + strand at 284.572 kb on - strand at 284.572 kb on - strand at 284.572 kb on - strand at 284.654 kb on + strand, within GFF553 at 284.781 kb on + strand at 284.788 kb on + strand at 284.806 kb on - strand at 284.884 kb on - strand at 285.009 kb on + strand, within GFF554 at 285.013 kb on + strand, within GFF554 at 285.013 kb on + strand, within GFF554 at 285.017 kb on - strand, within GFF554 at 285.107 kb on - strand, within GFF554 at 285.132 kb on + strand at 285.204 kb on + strand at 285.204 kb on + strand at 285.212 kb on + strand at 285.213 kb on - strand at 285.216 kb on + strand at 285.284 kb on - strand at 285.395 kb on + strand at 285.395 kb on + strand at 285.418 kb on + strand at 285.426 kb on + strand at 285.581 kb on + strand, within GFF555 at 285.581 kb on + strand, within GFF555 at 285.583 kb on + strand, within GFF555 at 285.593 kb on + strand, within GFF555 at 285.593 kb on + strand, within GFF555 at 285.593 kb on + strand, within GFF555 at 285.593 kb on + strand, within GFF555 at 285.594 kb on - strand, within GFF555 at 285.594 kb on - strand, within GFF555 at 285.594 kb on - strand, within GFF555 at 285.652 kb on - strand, within GFF555 at 285.652 kb on - strand, within GFF555 at 285.662 kb on + strand, within GFF555 at 285.736 kb on + strand, within GFF555 at 285.739 kb on + strand, within GFF555 at 285.739 kb on + strand, within GFF555 at 285.779 kb on + strand, within GFF555 at 285.783 kb on - strand, within GFF555 at 285.783 kb on - strand, within GFF555 at 285.824 kb on - strand, within GFF555 at 285.849 kb on - strand, within GFF555 at 285.923 kb on + strand, within GFF555 at 285.923 kb on + strand, within GFF555 at 285.924 kb on - strand, within GFF555 at 286.142 kb on + strand, within GFF555
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 283,908 + GFF552 0.28 +0.5 283,917 - GFF552 0.29 +0.1 284,059 - GFF552 0.49 -0.6 284,072 - GFF552 0.51 +1.9 284,183 + GFF552 0.66 +0.1 284,184 - GFF552 0.66 -0.0 284,410 + +0.5 284,411 - +0.5 284,530 - +0.1 284,538 - +0.4 284,571 + +1.9 284,572 - +1.2 284,572 - +0.9 284,572 - +0.6 284,654 + GFF553 0.44 +0.5 284,781 + -1.4 284,788 + -0.4 284,806 - +2.7 284,884 - +0.7 285,009 + GFF554 0.48 +0.5 285,013 + GFF554 0.50 +0.8 285,013 + GFF554 0.50 +2.1 285,017 - GFF554 0.51 -0.6 285,107 - GFF554 0.86 -0.1 285,132 + -1.1 285,204 + -0.2 285,204 + +0.3 285,212 + +1.6 285,213 - -0.1 285,216 + -0.8 285,284 - -0.1 285,395 + -1.2 285,395 + +1.1 285,418 + +0.5 285,426 + +0.1 285,581 + GFF555 0.14 +1.1 285,581 + GFF555 0.14 +0.1 285,583 + GFF555 0.14 +0.1 285,593 + GFF555 0.15 +0.9 285,593 + GFF555 0.15 +0.7 285,593 + GFF555 0.15 +1.4 285,593 + GFF555 0.15 +0.1 285,594 - GFF555 0.15 -0.5 285,594 - GFF555 0.15 +3.4 285,594 - GFF555 0.15 +1.2 285,652 - GFF555 0.19 -0.8 285,652 - GFF555 0.19 -1.0 285,662 + GFF555 0.20 +2.1 285,736 + GFF555 0.25 +0.9 285,739 + GFF555 0.26 -0.7 285,739 + GFF555 0.26 +2.1 285,779 + GFF555 0.29 -0.5 285,783 - GFF555 0.29 +0.6 285,783 - GFF555 0.29 +1.6 285,824 - GFF555 0.32 +0.2 285,849 - GFF555 0.34 +0.0 285,923 + GFF555 0.40 +2.3 285,923 + GFF555 0.40 +0.2 285,924 - GFF555 0.40 -1.0 286,142 + GFF555 0.56 +2.5
Or see this region's nucleotide sequence