Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF516 and GFF517 are separated by 181 nucleotides GFF517 and GFF518 are separated by 6 nucleotides GFF518 and GFF519 are separated by 91 nucleotides
GFF516 - Putative phosphatase, at 245,643 to 246,914
GFF516
GFF517 - Oligopeptidase A (EC 3.4.24.70), at 247,096 to 249,138
GFF517
GFF518 - rRNA small subunit methyltransferase J, at 249,145 to 249,903
GFF518
GFF519 - Di/tripeptide permease DtpB, at 249,995 to 251,413
GFF519
Position (kb) 247
248
249
250 Strain fitness (log2 ratio) -1
0
1
2 at 246.144 kb on + strand, within GFF516 at 246.144 kb on + strand, within GFF516 at 246.149 kb on + strand, within GFF516 at 246.182 kb on + strand, within GFF516 at 246.257 kb on + strand, within GFF516 at 246.257 kb on + strand, within GFF516 at 246.257 kb on + strand, within GFF516 at 246.257 kb on + strand, within GFF516 at 246.258 kb on - strand, within GFF516 at 246.258 kb on - strand, within GFF516 at 246.356 kb on + strand, within GFF516 at 246.356 kb on + strand, within GFF516 at 246.356 kb on + strand, within GFF516 at 246.455 kb on - strand, within GFF516 at 246.500 kb on + strand, within GFF516 at 246.501 kb on - strand, within GFF516 at 246.514 kb on - strand, within GFF516 at 246.519 kb on - strand, within GFF516 at 246.536 kb on + strand, within GFF516 at 246.537 kb on - strand, within GFF516 at 246.659 kb on + strand, within GFF516 at 246.659 kb on + strand, within GFF516 at 246.666 kb on + strand, within GFF516 at 246.684 kb on + strand, within GFF516 at 246.684 kb on + strand, within GFF516 at 246.684 kb on + strand, within GFF516 at 246.685 kb on - strand, within GFF516 at 246.956 kb on - strand at 246.956 kb on - strand at 246.968 kb on + strand at 246.992 kb on - strand at 247.112 kb on + strand at 247.113 kb on - strand at 247.113 kb on - strand at 247.206 kb on - strand at 247.206 kb on - strand at 247.237 kb on - strand at 247.237 kb on - strand at 247.290 kb on + strand at 247.351 kb on + strand, within GFF517 at 247.414 kb on + strand, within GFF517 at 247.599 kb on + strand, within GFF517 at 247.848 kb on + strand, within GFF517 at 247.849 kb on - strand, within GFF517 at 247.927 kb on + strand, within GFF517 at 248.105 kb on - strand, within GFF517 at 248.132 kb on - strand, within GFF517 at 248.153 kb on + strand, within GFF517 at 248.162 kb on - strand, within GFF517 at 248.238 kb on + strand, within GFF517 at 248.295 kb on + strand, within GFF517 at 248.329 kb on + strand, within GFF517 at 248.659 kb on + strand, within GFF517 at 248.720 kb on - strand, within GFF517 at 248.736 kb on + strand, within GFF517 at 248.737 kb on - strand, within GFF517 at 248.935 kb on + strand at 248.936 kb on - strand at 249.192 kb on - strand at 249.649 kb on + strand, within GFF518 at 249.874 kb on - strand at 249.922 kb on + strand at 249.951 kb on - strand at 249.997 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 246,144 + GFF516 0.39 +0.8 246,144 + GFF516 0.39 +0.5 246,149 + GFF516 0.40 +0.1 246,182 + GFF516 0.42 +0.1 246,257 + GFF516 0.48 +1.5 246,257 + GFF516 0.48 -0.4 246,257 + GFF516 0.48 +1.8 246,257 + GFF516 0.48 -0.8 246,258 - GFF516 0.48 -0.7 246,258 - GFF516 0.48 -0.6 246,356 + GFF516 0.56 +0.9 246,356 + GFF516 0.56 -0.2 246,356 + GFF516 0.56 +0.9 246,455 - GFF516 0.64 +0.4 246,500 + GFF516 0.67 -1.7 246,501 - GFF516 0.67 +0.8 246,514 - GFF516 0.68 -1.7 246,519 - GFF516 0.69 -1.2 246,536 + GFF516 0.70 +0.2 246,537 - GFF516 0.70 +1.2 246,659 + GFF516 0.80 -0.4 246,659 + GFF516 0.80 +0.7 246,666 + GFF516 0.80 -0.2 246,684 + GFF516 0.82 -0.2 246,684 + GFF516 0.82 +0.6 246,684 + GFF516 0.82 -0.0 246,685 - GFF516 0.82 -1.7 246,956 - -0.3 246,956 - +0.1 246,968 + +0.2 246,992 - +0.3 247,112 + +1.2 247,113 - +2.4 247,113 - +1.4 247,206 - +0.9 247,206 - +1.7 247,237 - +0.5 247,237 - +2.5 247,290 + -0.1 247,351 + GFF517 0.12 +0.4 247,414 + GFF517 0.16 +0.6 247,599 + GFF517 0.25 +1.8 247,848 + GFF517 0.37 +0.6 247,849 - GFF517 0.37 +0.4 247,927 + GFF517 0.41 +1.7 248,105 - GFF517 0.49 +1.2 248,132 - GFF517 0.51 +1.8 248,153 + GFF517 0.52 +1.4 248,162 - GFF517 0.52 +0.7 248,238 + GFF517 0.56 +2.5 248,295 + GFF517 0.59 +1.1 248,329 + GFF517 0.60 +1.1 248,659 + GFF517 0.77 +1.3 248,720 - GFF517 0.79 +1.3 248,736 + GFF517 0.80 +2.5 248,737 - GFF517 0.80 +1.9 248,935 + +1.4 248,936 - +1.3 249,192 - -0.3 249,649 + GFF518 0.66 -1.1 249,874 - -0.4 249,922 + +0.0 249,951 - +0.4 249,997 - +0.6
Or see this region's nucleotide sequence