Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4897 and GFF4898 are separated by 236 nucleotides
GFF4897 - Seryl-tRNA synthetase (EC 6.1.1.11), at 18,036 to 19,328
GFF4897
GFF4898 - Anaerobic dimethyl sulfoxide reductase chain A (EC 1.8.99.-), at 19,565 to 22,009
GFF4898
Position (kb) 19
20
21
22
23 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 19.573 kb on + strand at 19.753 kb on + strand at 19.754 kb on - strand at 19.786 kb on + strand at 19.786 kb on + strand at 19.786 kb on + strand at 19.819 kb on - strand, within GFF4898 at 19.847 kb on + strand, within GFF4898 at 19.892 kb on - strand, within GFF4898 at 19.907 kb on + strand, within GFF4898 at 19.907 kb on + strand, within GFF4898 at 19.910 kb on - strand, within GFF4898 at 19.976 kb on - strand, within GFF4898 at 19.979 kb on - strand, within GFF4898 at 20.015 kb on - strand, within GFF4898 at 20.208 kb on - strand, within GFF4898 at 20.214 kb on - strand, within GFF4898 at 20.260 kb on + strand, within GFF4898 at 20.320 kb on - strand, within GFF4898 at 20.328 kb on - strand, within GFF4898 at 20.469 kb on - strand, within GFF4898 at 20.513 kb on - strand, within GFF4898 at 20.583 kb on - strand, within GFF4898 at 20.585 kb on - strand, within GFF4898 at 20.641 kb on + strand, within GFF4898 at 20.812 kb on + strand, within GFF4898 at 20.857 kb on + strand, within GFF4898 at 20.857 kb on + strand, within GFF4898 at 20.971 kb on + strand, within GFF4898 at 21.005 kb on + strand, within GFF4898 at 21.109 kb on + strand, within GFF4898 at 21.209 kb on + strand, within GFF4898 at 21.253 kb on + strand, within GFF4898 at 21.457 kb on + strand, within GFF4898 at 21.494 kb on - strand, within GFF4898 at 21.618 kb on - strand, within GFF4898 at 21.619 kb on + strand, within GFF4898 at 21.681 kb on + strand, within GFF4898 at 21.692 kb on + strand, within GFF4898 at 21.692 kb on + strand, within GFF4898 at 21.692 kb on + strand, within GFF4898 at 21.817 kb on - strand at 21.820 kb on + strand at 21.884 kb on + strand at 21.931 kb on - strand at 21.979 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 19,573 + -0.1 19,753 + +0.8 19,754 - +1.0 19,786 + +0.7 19,786 + -0.3 19,786 + +0.2 19,819 - GFF4898 0.10 +0.1 19,847 + GFF4898 0.12 +1.9 19,892 - GFF4898 0.13 -2.3 19,907 + GFF4898 0.14 +0.5 19,907 + GFF4898 0.14 +0.2 19,910 - GFF4898 0.14 -1.2 19,976 - GFF4898 0.17 -0.0 19,979 - GFF4898 0.17 -0.2 20,015 - GFF4898 0.18 +0.2 20,208 - GFF4898 0.26 -1.2 20,214 - GFF4898 0.27 +0.1 20,260 + GFF4898 0.28 -0.6 20,320 - GFF4898 0.31 +0.8 20,328 - GFF4898 0.31 +0.6 20,469 - GFF4898 0.37 +0.5 20,513 - GFF4898 0.39 -1.2 20,583 - GFF4898 0.42 -2.1 20,585 - GFF4898 0.42 +0.1 20,641 + GFF4898 0.44 +0.9 20,812 + GFF4898 0.51 +0.7 20,857 + GFF4898 0.53 +0.3 20,857 + GFF4898 0.53 +0.7 20,971 + GFF4898 0.58 +0.2 21,005 + GFF4898 0.59 +0.7 21,109 + GFF4898 0.63 -0.5 21,209 + GFF4898 0.67 +1.1 21,253 + GFF4898 0.69 +3.1 21,457 + GFF4898 0.77 +0.1 21,494 - GFF4898 0.79 +0.7 21,618 - GFF4898 0.84 -0.2 21,619 + GFF4898 0.84 +0.3 21,681 + GFF4898 0.87 +3.4 21,692 + GFF4898 0.87 -1.5 21,692 + GFF4898 0.87 +1.1 21,692 + GFF4898 0.87 +0.3 21,817 - +0.0 21,820 + +0.5 21,884 + +0.6 21,931 - +1.9 21,979 + +0.4
Or see this region's nucleotide sequence