Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4820 and GFF4821 are separated by 324 nucleotides GFF4821 and GFF4822 are separated by 30 nucleotides GFF4822 and GFF4823 are separated by 87 nucleotides GFF4823 and GFF4824 overlap by 2 nucleotides GFF4824 and GFF4825 are separated by 326 nucleotides
GFF4820 - Sulfite reductase [NADPH] flavoprotein alpha-component (EC 1.8.1.2), at 120,677 to 122,476
GFF4820
GFF4821 - FIG01047384: hypothetical protein, at 122,801 to 122,971
GFF4821
GFF4822 - Queuosine biosynthesis QueD, PTPS-I, at 123,002 to 123,262
GFF4822
GFF4823 - STY3078 protein, at 123,350 to 124,147
GFF4823
GFF4824 - FIG01046827: hypothetical protein, at 124,146 to 124,400
GFF4824
GFF4825 - hypothetical protein, at 124,727 to 124,963
GFF4825
Position (kb)
123
124
125 Strain fitness (log2 ratio)
-1
0
1
2 at 122.484 kb on + strand at 122.561 kb on - strand at 122.561 kb on - strand at 122.917 kb on + strand, within GFF4821 at 122.921 kb on + strand, within GFF4821 at 122.972 kb on - strand at 123.092 kb on + strand, within GFF4822 at 123.114 kb on - strand, within GFF4822 at 123.575 kb on - strand, within GFF4823 at 123.634 kb on - strand, within GFF4823 at 123.793 kb on - strand, within GFF4823 at 123.934 kb on - strand, within GFF4823 at 124.037 kb on - strand, within GFF4823 at 124.037 kb on - strand, within GFF4823 at 124.128 kb on - strand at 124.208 kb on + strand, within GFF4824 at 124.239 kb on + strand, within GFF4824 at 124.251 kb on - strand, within GFF4824 at 124.312 kb on - strand, within GFF4824 at 124.397 kb on - strand at 124.399 kb on - strand at 124.399 kb on - strand at 124.402 kb on - strand at 124.601 kb on + strand at 124.701 kb on + strand at 124.962 kb on - strand at 125.007 kb on + strand at 125.088 kb on - strand at 125.088 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 122,484 + +0.7 122,561 - -0.4 122,561 - +0.2 122,917 + GFF4821 0.68 +0.4 122,921 + GFF4821 0.70 -0.2 122,972 - +0.2 123,092 + GFF4822 0.34 +1.5 123,114 - GFF4822 0.43 -1.3 123,575 - GFF4823 0.28 -0.8 123,634 - GFF4823 0.36 -0.1 123,793 - GFF4823 0.56 +0.1 123,934 - GFF4823 0.73 -0.4 124,037 - GFF4823 0.86 -1.4 124,037 - GFF4823 0.86 +2.5 124,128 - +1.2 124,208 + GFF4824 0.24 +1.5 124,239 + GFF4824 0.36 +1.5 124,251 - GFF4824 0.41 +0.6 124,312 - GFF4824 0.65 -0.2 124,397 - +0.3 124,399 - +0.9 124,399 - +0.2 124,402 - +0.2 124,601 + +2.3 124,701 + -1.5 124,962 - +0.2 125,007 + -0.0 125,088 - -0.7 125,088 - -0.2
Or see this region's nucleotide sequence