Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4609 and GFF4610 are separated by 20 nucleotides GFF4610 and GFF4611 are separated by 425 nucleotides GFF4611 and GFF4612 are separated by 110 nucleotides
GFF4609 - Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1), at 400,831 to 402,879
GFF4609
GFF4610 - Potassium-transporting ATPase A chain (EC 3.6.3.12) (TC 3.A.3.7.1), at 402,900 to 404,579
GFF4610
GFF4611 - FIG01200701: possible membrane protein, at 405,005 to 405,211
GFF4611
GFF4612 - Deoxyribodipyrimidine photolyase (EC 4.1.99.3), at 405,322 to 406,743
GFF4612
Position (kb) 402
403
404
405 Strain fitness (log2 ratio) -2
-1
0
1
2 at 401.946 kb on + strand, within GFF4609 at 401.946 kb on + strand, within GFF4609 at 402.687 kb on + strand at 402.719 kb on - strand at 402.879 kb on + strand at 402.887 kb on + strand at 402.888 kb on - strand at 402.950 kb on + strand at 402.998 kb on + strand at 403.209 kb on + strand, within GFF4610 at 403.209 kb on + strand, within GFF4610 at 403.210 kb on - strand, within GFF4610 at 403.210 kb on - strand, within GFF4610 at 403.210 kb on - strand, within GFF4610 at 403.439 kb on - strand, within GFF4610 at 403.439 kb on - strand, within GFF4610 at 403.540 kb on - strand, within GFF4610 at 404.046 kb on + strand, within GFF4610 at 404.227 kb on + strand, within GFF4610 at 404.244 kb on - strand, within GFF4610 at 404.314 kb on - strand, within GFF4610 at 404.391 kb on + strand, within GFF4610 at 404.392 kb on - strand, within GFF4610 at 404.694 kb on - strand at 404.843 kb on + strand at 404.964 kb on + strand at 404.989 kb on + strand at 405.004 kb on + strand at 405.014 kb on + strand at 405.128 kb on + strand, within GFF4611 at 405.128 kb on + strand, within GFF4611 at 405.207 kb on - strand at 405.273 kb on + strand at 405.274 kb on - strand at 405.274 kb on - strand at 405.321 kb on + strand at 405.322 kb on - strand at 405.322 kb on - strand at 405.322 kb on - strand at 405.335 kb on + strand at 405.426 kb on + strand at 405.426 kb on + strand at 405.427 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 401,946 + GFF4609 0.54 -0.0 401,946 + GFF4609 0.54 -0.6 402,687 + -2.1 402,719 - +0.6 402,879 + -1.1 402,887 + -0.1 402,888 - +0.0 402,950 + +0.8 402,998 + -1.3 403,209 + GFF4610 0.18 -0.5 403,209 + GFF4610 0.18 +1.6 403,210 - GFF4610 0.18 +0.8 403,210 - GFF4610 0.18 +0.2 403,210 - GFF4610 0.18 +1.5 403,439 - GFF4610 0.32 -1.4 403,439 - GFF4610 0.32 +1.7 403,540 - GFF4610 0.38 +0.3 404,046 + GFF4610 0.68 +0.5 404,227 + GFF4610 0.79 -0.1 404,244 - GFF4610 0.80 -0.1 404,314 - GFF4610 0.84 +0.2 404,391 + GFF4610 0.89 +1.8 404,392 - GFF4610 0.89 -0.1 404,694 - +0.1 404,843 + -1.0 404,964 + -0.5 404,989 + -1.8 405,004 + +0.4 405,014 + +0.7 405,128 + GFF4611 0.59 +0.0 405,128 + GFF4611 0.59 +1.0 405,207 - +0.1 405,273 + +1.0 405,274 - -0.1 405,274 - -2.6 405,321 + -0.0 405,322 - +0.8 405,322 - -1.0 405,322 - -0.3 405,335 + -0.4 405,426 + +0.2 405,426 + -0.4 405,427 - +0.2
Or see this region's nucleotide sequence