Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4440 and GFF4441 are separated by 2 nucleotides GFF4441 and GFF4442 are separated by 172 nucleotides GFF4442 and GFF4443 are separated by 43 nucleotides GFF4443 and GFF4444 overlap by 4 nucleotides
GFF4440 - UDP-2,3-diacylglucosamine diphosphatase (EC 3.6.1.54), at 227,957 to 228,679
GFF4440
GFF4441 - Peptidyl-prolyl cis-trans isomerase PpiB (EC 5.2.1.8), at 228,682 to 229,176
GFF4441
GFF4442 - Cysteinyl-tRNA synthetase (EC 6.1.1.16), at 229,349 to 230,734
GFF4442
GFF4443 - Putative outer membrane protein, at 230,778 to 231,602
GFF4443
GFF4444 - Putative inner membrane protein, at 231,599 to 232,036
GFF4444
Position (kb) 229
230
231 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 229.228 kb on - strand at 229.228 kb on - strand at 229.228 kb on - strand at 229.228 kb on - strand at 229.228 kb on - strand at 229.228 kb on - strand at 229.228 kb on - strand at 229.230 kb on - strand at 229.230 kb on - strand at 229.248 kb on - strand at 229.297 kb on - strand at 229.297 kb on - strand at 229.297 kb on - strand at 229.303 kb on + strand at 230.755 kb on - strand at 230.870 kb on + strand, within GFF4443 at 230.899 kb on - strand, within GFF4443 at 231.051 kb on - strand, within GFF4443 at 231.051 kb on - strand, within GFF4443 at 231.144 kb on + strand, within GFF4443 at 231.150 kb on - strand, within GFF4443 at 231.188 kb on + strand, within GFF4443 at 231.193 kb on + strand, within GFF4443 at 231.291 kb on + strand, within GFF4443 at 231.291 kb on + strand, within GFF4443 at 231.292 kb on - strand, within GFF4443 at 231.292 kb on - strand, within GFF4443 at 231.293 kb on + strand, within GFF4443 at 231.295 kb on + strand, within GFF4443 at 231.295 kb on + strand, within GFF4443 at 231.410 kb on + strand, within GFF4443 at 231.536 kb on + strand at 231.537 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 229,228 - +0.4 229,228 - -0.8 229,228 - -0.8 229,228 - -0.4 229,228 - -0.6 229,228 - -0.1 229,228 - -0.1 229,230 - +3.7 229,230 - -0.0 229,248 - -0.6 229,297 - -0.2 229,297 - -1.1 229,297 - -0.2 229,303 + -1.6 230,755 - +0.0 230,870 + GFF4443 0.11 +0.7 230,899 - GFF4443 0.15 -0.6 231,051 - GFF4443 0.33 -0.1 231,051 - GFF4443 0.33 -0.5 231,144 + GFF4443 0.44 +0.5 231,150 - GFF4443 0.45 -0.0 231,188 + GFF4443 0.50 +0.5 231,193 + GFF4443 0.50 -0.3 231,291 + GFF4443 0.62 +0.2 231,291 + GFF4443 0.62 +0.1 231,292 - GFF4443 0.62 -0.4 231,292 - GFF4443 0.62 +1.3 231,293 + GFF4443 0.62 -0.7 231,295 + GFF4443 0.63 -2.1 231,295 + GFF4443 0.63 +2.1 231,410 + GFF4443 0.77 +2.7 231,536 + -1.4 231,537 - +0.3
Or see this region's nucleotide sequence