Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4435 and GFF4436 are separated by 32 nucleotides GFF4436 and GFF4437 are separated by 3 nucleotides GFF4437 and GFF4438 are separated by 40 nucleotides
GFF4435 - FIG074102: hypothetical protein, at 223,248 to 224,507
GFF4435
GFF4436 - FIG074102: hypothetical protein, at 224,540 to 225,328
GFF4436
GFF4437 - Carbamate kinase (EC 2.7.2.2), at 225,332 to 226,225
GFF4437
GFF4438 - Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21), at 226,266 to 227,333
GFF4438
Position (kb) 224
225
226 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 223.560 kb on - strand, within GFF4435 at 223.560 kb on - strand, within GFF4435 at 223.723 kb on + strand, within GFF4435 at 223.724 kb on - strand, within GFF4435 at 224.030 kb on + strand, within GFF4435 at 224.402 kb on + strand at 224.527 kb on + strand at 224.608 kb on + strand at 224.634 kb on + strand, within GFF4436 at 224.853 kb on - strand, within GFF4436 at 224.917 kb on - strand, within GFF4436 at 225.176 kb on + strand, within GFF4436 at 225.346 kb on - strand at 225.369 kb on + strand at 225.415 kb on + strand at 225.415 kb on + strand at 225.415 kb on + strand at 225.471 kb on + strand, within GFF4437 at 225.475 kb on - strand, within GFF4437 at 225.475 kb on - strand, within GFF4437 at 225.589 kb on + strand, within GFF4437 at 225.589 kb on + strand, within GFF4437 at 225.713 kb on + strand, within GFF4437 at 225.749 kb on + strand, within GFF4437 at 225.779 kb on + strand, within GFF4437 at 225.931 kb on - strand, within GFF4437 at 226.013 kb on + strand, within GFF4437 at 226.014 kb on - strand, within GFF4437 at 226.267 kb on + strand at 226.271 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 223,560 - GFF4435 0.25 -0.2 223,560 - GFF4435 0.25 -0.7 223,723 + GFF4435 0.38 +0.7 223,724 - GFF4435 0.38 -2.3 224,030 + GFF4435 0.62 -0.4 224,402 + +1.2 224,527 + +1.3 224,608 + -2.8 224,634 + GFF4436 0.12 +1.0 224,853 - GFF4436 0.40 +2.2 224,917 - GFF4436 0.48 +0.3 225,176 + GFF4436 0.81 +0.8 225,346 - -0.8 225,369 + +0.3 225,415 + +3.8 225,415 + +0.9 225,415 + -1.6 225,471 + GFF4437 0.16 -1.6 225,475 - GFF4437 0.16 -1.1 225,475 - GFF4437 0.16 -0.0 225,589 + GFF4437 0.29 -2.5 225,589 + GFF4437 0.29 -0.9 225,713 + GFF4437 0.43 +0.6 225,749 + GFF4437 0.47 -1.1 225,779 + GFF4437 0.50 -0.1 225,931 - GFF4437 0.67 +0.4 226,013 + GFF4437 0.76 -0.6 226,014 - GFF4437 0.76 -0.3 226,267 + -0.6 226,271 + -0.1
Or see this region's nucleotide sequence