Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4404 and GFF4405 are separated by 109 nucleotides GFF4405 and GFF4406 are separated by 0 nucleotides GFF4406 and GFF4407 overlap by 4 nucleotides GFF4407 and GFF4408 are separated by 146 nucleotides GFF4408 and GFF4409 overlap by 14 nucleotides
GFF4404 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 188,159 to 190,660
GFF4404
GFF4405 - HTH-type transcriptional regulator cueR, at 190,770 to 191,186
GFF4405
GFF4406 - Putative activity regulator of membrane protease YbbK, at 191,187 to 191,639
GFF4406
GFF4407 - Putative stomatin/prohibitin-family membrane protease subunit YbbK, at 191,636 to 192,553
GFF4407
GFF4408 - YbbL ABC transporter ATP-binding protein, at 192,700 to 193,377
GFF4408
GFF4409 - YbbM seven transmembrane helix protein, at 193,364 to 194,143
GFF4409
Position (kb) 191
192
193 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 190.649 kb on - strand at 190.661 kb on - strand at 190.702 kb on + strand at 190.705 kb on + strand at 190.769 kb on + strand at 190.804 kb on + strand at 190.858 kb on + strand, within GFF4405 at 190.859 kb on - strand, within GFF4405 at 190.859 kb on - strand, within GFF4405 at 190.944 kb on - strand, within GFF4405 at 191.142 kb on - strand, within GFF4405 at 191.243 kb on + strand, within GFF4406 at 191.336 kb on + strand, within GFF4406 at 191.802 kb on - strand, within GFF4407 at 191.978 kb on + strand, within GFF4407 at 192.130 kb on + strand, within GFF4407 at 192.258 kb on + strand, within GFF4407 at 192.535 kb on + strand at 192.536 kb on - strand at 192.612 kb on - strand at 192.647 kb on - strand at 192.659 kb on - strand at 192.659 kb on - strand at 192.712 kb on - strand at 192.912 kb on + strand, within GFF4408 at 192.935 kb on - strand, within GFF4408 at 192.952 kb on + strand, within GFF4408 at 192.992 kb on - strand, within GFF4408 at 192.992 kb on - strand, within GFF4408 at 193.134 kb on + strand, within GFF4408 at 193.135 kb on - strand, within GFF4408 at 193.149 kb on + strand, within GFF4408 at 193.213 kb on - strand, within GFF4408 at 193.236 kb on + strand, within GFF4408 at 193.375 kb on + strand at 193.376 kb on - strand at 193.378 kb on + strand at 193.378 kb on + strand at 193.378 kb on + strand at 193.378 kb on + strand at 193.388 kb on - strand at 193.482 kb on + strand, within GFF4409 at 193.483 kb on - strand, within GFF4409
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 190,649 - +0.1 190,661 - +4.2 190,702 + +4.3 190,705 + -1.8 190,769 + +1.6 190,804 + +0.5 190,858 + GFF4405 0.21 +0.2 190,859 - GFF4405 0.21 +0.9 190,859 - GFF4405 0.21 +0.9 190,944 - GFF4405 0.42 +0.4 191,142 - GFF4405 0.89 +3.6 191,243 + GFF4406 0.12 +0.9 191,336 + GFF4406 0.33 +1.1 191,802 - GFF4407 0.18 -0.8 191,978 + GFF4407 0.37 -1.4 192,130 + GFF4407 0.54 -0.2 192,258 + GFF4407 0.68 -0.4 192,535 + -0.6 192,536 - +0.2 192,612 - +0.1 192,647 - +1.1 192,659 - +0.2 192,659 - -0.2 192,712 - -1.1 192,912 + GFF4408 0.31 -0.2 192,935 - GFF4408 0.35 +0.9 192,952 + GFF4408 0.37 -0.1 192,992 - GFF4408 0.43 +1.8 192,992 - GFF4408 0.43 -0.2 193,134 + GFF4408 0.64 +0.5 193,135 - GFF4408 0.64 -0.7 193,149 + GFF4408 0.66 -0.9 193,213 - GFF4408 0.76 -0.3 193,236 + GFF4408 0.79 -0.8 193,375 + +2.0 193,376 - +0.0 193,378 + -1.1 193,378 + -1.7 193,378 + +1.2 193,378 + -0.4 193,388 - +1.8 193,482 + GFF4409 0.15 +1.7 193,483 - GFF4409 0.15 +0.9
Or see this region's nucleotide sequence