Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4305

Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4304 and GFF4305 are separated by 196 nucleotidesGFF4305 and GFF4306 are separated by 22 nucleotidesGFF4306 and GFF4307 are separated by 60 nucleotides GFF4304 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-), at 89,772 to 90,836 GFF4304 GFF4305 - tRNA-guanine transglycosylase (EC 2.4.2.29), at 91,033 to 92,160 GFF4305 GFF4306 - Preprotein translocase subunit YajC (TC 3.A.5.1.1), at 92,183 to 92,515 GFF4306 GFF4307 - Protein-export membrane protein SecD (TC 3.A.5.1.1), at 92,576 to 94,390 GFF4307 Position (kb) 91 92 93Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 90.225 kb on + strand, within GFF4304at 90.226 kb on - strand, within GFF4304at 90.589 kb on + strand, within GFF4304at 90.624 kb on + strand, within GFF4304at 90.633 kb on + strand, within GFF4304at 90.741 kb on + strandat 90.743 kb on + strandat 90.744 kb on - strandat 90.744 kb on - strandat 90.744 kb on - strandat 90.754 kb on + strandat 90.776 kb on + strandat 90.804 kb on + strandat 90.804 kb on + strandat 90.805 kb on - strandat 90.891 kb on - strandat 90.967 kb on + strandat 90.967 kb on + strandat 91.031 kb on + strandat 91.046 kb on + strandat 91.119 kb on + strandat 91.135 kb on + strandat 91.136 kb on - strandat 91.493 kb on - strand, within GFF4305at 91.498 kb on - strand, within GFF4305at 91.701 kb on + strand, within GFF4305at 91.815 kb on + strand, within GFF4305at 91.815 kb on + strand, within GFF4305at 91.905 kb on + strand, within GFF4305at 92.167 kb on + strandat 92.475 kb on + strand, within GFF4306

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
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90,225 + GFF4304 0.43 -0.5
90,226 - GFF4304 0.43 -0.6
90,589 + GFF4304 0.77 +0.4
90,624 + GFF4304 0.80 -0.5
90,633 + GFF4304 0.81 +0.6
90,741 + -0.4
90,743 + +2.4
90,744 - -0.8
90,744 - -0.4
90,744 - +0.0
90,754 + +0.0
90,776 + -1.4
90,804 + -0.6
90,804 + -0.6
90,805 - -0.4
90,891 - -0.8
90,967 + +0.3
90,967 + +2.1
91,031 + -1.5
91,046 + +0.4
91,119 + -0.8
91,135 + +0.1
91,136 - +1.2
91,493 - GFF4305 0.41 -1.2
91,498 - GFF4305 0.41 +1.1
91,701 + GFF4305 0.59 -1.0
91,815 + GFF4305 0.69 -1.4
91,815 + GFF4305 0.69 -0.7
91,905 + GFF4305 0.77 -0.5
92,167 + -2.4
92,475 + GFF4306 0.88 +5.5

Or see this region's nucleotide sequence