Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF417 and GFF418 are separated by 220 nucleotides GFF418 and GFF419 are separated by 475 nucleotides
GFF417 - Mannitol-1-phosphate 5-dehydrogenase (EC 1.1.1.17), at 134,526 to 135,674
GFF417
GFF418 - PTS system, mannitol-specific IIC component (EC 2.7.1.69) / PTS system, mannitol-specific IIB component (EC 2.7.1.69) / PTS system, mannitol-specific IIA component, at 135,895 to 137,811
GFF418
GFF419 - Uncharacterized GST-like protein yibF, at 138,287 to 138,895
GFF419
Position (kb) 135
136
137
138 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 135.011 kb on + strand, within GFF417 at 135.011 kb on + strand, within GFF417 at 135.011 kb on + strand, within GFF417 at 135.012 kb on - strand, within GFF417 at 135.041 kb on + strand, within GFF417 at 135.041 kb on - strand, within GFF417 at 135.108 kb on - strand, within GFF417 at 135.108 kb on - strand, within GFF417 at 135.197 kb on + strand, within GFF417 at 135.326 kb on + strand, within GFF417 at 135.327 kb on - strand, within GFF417 at 135.393 kb on - strand, within GFF417 at 135.430 kb on - strand, within GFF417 at 135.430 kb on - strand, within GFF417 at 135.430 kb on - strand, within GFF417 at 135.542 kb on + strand, within GFF417 at 135.543 kb on - strand, within GFF417 at 135.543 kb on - strand, within GFF417 at 135.579 kb on + strand at 135.737 kb on - strand at 135.737 kb on - strand at 135.820 kb on - strand at 135.823 kb on + strand at 135.823 kb on + strand at 135.824 kb on - strand at 135.824 kb on - strand at 135.827 kb on + strand at 135.827 kb on + strand at 135.828 kb on - strand at 135.828 kb on - strand at 135.828 kb on - strand at 135.889 kb on + strand at 135.890 kb on - strand at 136.023 kb on + strand at 136.024 kb on - strand at 136.082 kb on - strand at 136.178 kb on + strand, within GFF418 at 136.220 kb on + strand, within GFF418 at 136.221 kb on - strand, within GFF418 at 136.312 kb on - strand, within GFF418 at 136.341 kb on + strand, within GFF418 at 136.342 kb on - strand, within GFF418 at 136.396 kb on - strand, within GFF418 at 136.396 kb on - strand, within GFF418 at 136.396 kb on - strand, within GFF418 at 136.440 kb on - strand, within GFF418 at 136.528 kb on - strand, within GFF418 at 137.120 kb on + strand, within GFF418 at 137.316 kb on + strand, within GFF418 at 137.317 kb on - strand, within GFF418 at 137.560 kb on - strand, within GFF418 at 137.613 kb on + strand, within GFF418 at 137.640 kb on - strand at 137.700 kb on + strand at 137.715 kb on + strand at 137.742 kb on + strand at 137.745 kb on + strand at 137.757 kb on + strand at 137.758 kb on - strand at 137.827 kb on + strand at 137.827 kb on + strand at 137.827 kb on + strand at 137.870 kb on + strand at 137.901 kb on - strand at 137.901 kb on - strand at 137.905 kb on - strand at 137.954 kb on + strand at 137.973 kb on - strand at 137.994 kb on - strand at 137.994 kb on - strand at 138.006 kb on - strand at 138.251 kb on - strand at 138.251 kb on - strand at 138.263 kb on - strand at 138.301 kb on + strand at 138.356 kb on - strand, within GFF419 at 138.358 kb on + strand, within GFF419 at 138.358 kb on + strand, within GFF419 at 138.359 kb on - strand, within GFF419 at 138.386 kb on + strand, within GFF419 at 138.413 kb on + strand, within GFF419 at 138.462 kb on - strand, within GFF419 at 138.493 kb on + strand, within GFF419 at 138.493 kb on + strand, within GFF419 at 138.586 kb on + strand, within GFF419 at 138.587 kb on - strand, within GFF419 at 138.691 kb on + strand, within GFF419 at 138.692 kb on - strand, within GFF419
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 135,011 + GFF417 0.42 -1.0 135,011 + GFF417 0.42 -0.6 135,011 + GFF417 0.42 -1.9 135,012 - GFF417 0.42 +0.0 135,041 + GFF417 0.45 -2.2 135,041 - GFF417 0.45 +2.3 135,108 - GFF417 0.51 +0.1 135,108 - GFF417 0.51 +0.3 135,197 + GFF417 0.58 -1.1 135,326 + GFF417 0.70 -0.0 135,327 - GFF417 0.70 +0.1 135,393 - GFF417 0.75 +1.3 135,430 - GFF417 0.79 +0.7 135,430 - GFF417 0.79 +0.5 135,430 - GFF417 0.79 +0.6 135,542 + GFF417 0.88 +2.1 135,543 - GFF417 0.89 -1.6 135,543 - GFF417 0.89 +1.8 135,579 + -2.2 135,737 - +0.1 135,737 - +0.4 135,820 - +1.7 135,823 + +1.6 135,823 + +1.1 135,824 - -0.1 135,824 - -0.3 135,827 + +0.0 135,827 + -2.6 135,828 - +0.2 135,828 - +0.1 135,828 - -0.8 135,889 + +0.1 135,890 - -0.4 136,023 + +0.1 136,024 - -2.0 136,082 - +1.2 136,178 + GFF418 0.15 +0.2 136,220 + GFF418 0.17 +0.8 136,221 - GFF418 0.17 -1.1 136,312 - GFF418 0.22 +1.4 136,341 + GFF418 0.23 +2.3 136,342 - GFF418 0.23 +4.3 136,396 - GFF418 0.26 -0.1 136,396 - GFF418 0.26 -0.2 136,396 - GFF418 0.26 -0.5 136,440 - GFF418 0.28 -0.2 136,528 - GFF418 0.33 +2.7 137,120 + GFF418 0.64 +0.4 137,316 + GFF418 0.74 -0.9 137,317 - GFF418 0.74 -1.7 137,560 - GFF418 0.87 +1.7 137,613 + GFF418 0.90 +2.2 137,640 - +1.3 137,700 + +0.5 137,715 + -0.2 137,742 + -0.3 137,745 + -2.1 137,757 + +0.7 137,758 - -0.4 137,827 + -0.0 137,827 + -0.1 137,827 + +0.9 137,870 + +0.0 137,901 - +0.3 137,901 - +1.2 137,905 - +1.0 137,954 + -0.6 137,973 - -0.1 137,994 - -0.0 137,994 - +0.7 138,006 - -0.1 138,251 - +0.5 138,251 - +0.3 138,263 - +1.6 138,301 + -0.6 138,356 - GFF419 0.11 +0.3 138,358 + GFF419 0.12 -0.3 138,358 + GFF419 0.12 -0.9 138,359 - GFF419 0.12 +0.1 138,386 + GFF419 0.16 -0.5 138,413 + GFF419 0.21 -0.6 138,462 - GFF419 0.29 +0.6 138,493 + GFF419 0.34 -0.3 138,493 + GFF419 0.34 +0.6 138,586 + GFF419 0.49 +1.4 138,587 - GFF419 0.49 -0.7 138,691 + GFF419 0.66 -0.6 138,692 - GFF419 0.67 -0.4
Or see this region's nucleotide sequence