Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4013 and GFF4014 overlap by 4 nucleotides GFF4014 and GFF4015 are separated by 21 nucleotides GFF4015 and GFF4016 overlap by 1 nucleotides GFF4016 and GFF4017 overlap by 8 nucleotides
GFF4013 - Flagellar hook-length control protein FliK, at 281,895 to 283,112
GFF4013
GFF4014 - Flagellar protein FliJ, at 283,109 to 283,552
GFF4014
GFF4015 - Flagellum-specific ATP synthase FliI, at 283,574 to 284,944
GFF4015
GFF4016 - Flagellar assembly protein FliH, at 284,944 to 285,651
GFF4016
GFF4017 - Flagellar motor switch protein FliG, at 285,644 to 286,639
GFF4017
Position (kb) 283
284
285 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 282.752 kb on + strand, within GFF4013 at 282.916 kb on - strand, within GFF4013 at 282.928 kb on - strand, within GFF4013 at 283.500 kb on - strand, within GFF4014 at 283.679 kb on - strand at 283.771 kb on + strand, within GFF4015 at 283.791 kb on + strand, within GFF4015 at 284.115 kb on + strand, within GFF4015 at 284.495 kb on + strand, within GFF4015 at 284.708 kb on + strand, within GFF4015 at 284.728 kb on + strand, within GFF4015 at 284.852 kb on + strand at 285.033 kb on + strand, within GFF4016 at 285.033 kb on + strand, within GFF4016 at 285.646 kb on - strand at 285.646 kb on - strand at 285.651 kb on + strand at 285.651 kb on + strand at 285.651 kb on + strand at 285.651 kb on + strand at 285.651 kb on + strand at 285.655 kb on + strand at 285.655 kb on + strand at 285.709 kb on + strand at 285.808 kb on + strand, within GFF4017 at 285.808 kb on + strand, within GFF4017
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 282,752 + GFF4013 0.70 +0.2 282,916 - GFF4013 0.84 -0.1 282,928 - GFF4013 0.85 +1.6 283,500 - GFF4014 0.88 -2.7 283,679 - +1.0 283,771 + GFF4015 0.14 +3.5 283,791 + GFF4015 0.16 +0.6 284,115 + GFF4015 0.39 +0.3 284,495 + GFF4015 0.67 +0.8 284,708 + GFF4015 0.83 -0.1 284,728 + GFF4015 0.84 +0.5 284,852 + +1.8 285,033 + GFF4016 0.13 -0.4 285,033 + GFF4016 0.13 +0.3 285,646 - +0.5 285,646 - +1.1 285,651 + -0.1 285,651 + -1.0 285,651 + +0.1 285,651 + -0.8 285,651 + +0.6 285,655 + +0.2 285,655 + +0.5 285,709 + -1.2 285,808 + GFF4017 0.16 -1.1 285,808 + GFF4017 0.16 -0.8
Or see this region's nucleotide sequence