Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3919 and GFF3920 are separated by 618 nucleotides GFF3920 and GFF3921 are separated by 14 nucleotides GFF3921 and GFF3922 overlap by 4 nucleotides
GFF3919 - secreted effector protein, at 200,935 to 203,283
GFF3919
GFF3920 - Thiosulfate reductase precursor (EC 1.-.-.-), at 203,902 to 206,178
GFF3920
GFF3921 - Thiosulfate reductase electron transport protein phsB, at 206,193 to 206,771
GFF3921
GFF3922 - Thiosulfate reductase cytochrome B subunit, at 206,768 to 207,532
GFF3922
Position (kb) 203
204
205
206
207 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 203.910 kb on + strand at 204.083 kb on + strand at 204.252 kb on + strand, within GFF3920 at 204.397 kb on - strand, within GFF3920 at 204.399 kb on + strand, within GFF3920 at 204.646 kb on - strand, within GFF3920 at 204.666 kb on - strand, within GFF3920 at 204.826 kb on - strand, within GFF3920 at 204.871 kb on - strand, within GFF3920 at 205.059 kb on + strand, within GFF3920 at 205.059 kb on + strand, within GFF3920 at 205.418 kb on + strand, within GFF3920 at 205.495 kb on + strand, within GFF3920 at 205.495 kb on + strand, within GFF3920 at 205.681 kb on + strand, within GFF3920 at 205.814 kb on - strand, within GFF3920 at 205.842 kb on + strand, within GFF3920 at 205.861 kb on + strand, within GFF3920 at 205.861 kb on + strand, within GFF3920 at 205.862 kb on - strand, within GFF3920 at 205.866 kb on - strand, within GFF3920 at 205.883 kb on + strand, within GFF3920 at 205.884 kb on - strand, within GFF3920 at 206.035 kb on + strand at 206.035 kb on + strand at 206.243 kb on + strand at 206.262 kb on - strand, within GFF3921 at 206.312 kb on - strand, within GFF3921 at 206.481 kb on - strand, within GFF3921 at 206.561 kb on + strand, within GFF3921 at 206.763 kb on - strand at 206.774 kb on - strand at 206.776 kb on + strand at 206.793 kb on + strand at 206.793 kb on + strand at 206.937 kb on - strand, within GFF3922 at 206.998 kb on - strand, within GFF3922 at 207.051 kb on + strand, within GFF3922
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 203,910 + +1.8 204,083 + +1.4 204,252 + GFF3920 0.15 +0.5 204,397 - GFF3920 0.22 -2.2 204,399 + GFF3920 0.22 +0.2 204,646 - GFF3920 0.33 -0.1 204,666 - GFF3920 0.34 -1.2 204,826 - GFF3920 0.41 -0.1 204,871 - GFF3920 0.43 +0.8 205,059 + GFF3920 0.51 +0.8 205,059 + GFF3920 0.51 +0.6 205,418 + GFF3920 0.67 +0.3 205,495 + GFF3920 0.70 -0.4 205,495 + GFF3920 0.70 -1.1 205,681 + GFF3920 0.78 -0.3 205,814 - GFF3920 0.84 +2.2 205,842 + GFF3920 0.85 -0.8 205,861 + GFF3920 0.86 -1.4 205,861 + GFF3920 0.86 +0.2 205,862 - GFF3920 0.86 +0.3 205,866 - GFF3920 0.86 -0.1 205,883 + GFF3920 0.87 -0.4 205,884 - GFF3920 0.87 -0.6 206,035 + -0.3 206,035 + +1.4 206,243 + +0.6 206,262 - GFF3921 0.12 -0.8 206,312 - GFF3921 0.21 -0.6 206,481 - GFF3921 0.50 +0.5 206,561 + GFF3921 0.64 +1.0 206,763 - -0.5 206,774 - +3.0 206,776 + -0.5 206,793 + +0.8 206,793 + +1.9 206,937 - GFF3922 0.22 -0.8 206,998 - GFF3922 0.30 +0.8 207,051 + GFF3922 0.37 -0.1
Or see this region's nucleotide sequence