Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3906 and GFF3907 overlap by 7 nucleotides GFF3907 and GFF3908 overlap by 19 nucleotides GFF3908 and GFF3909 overlap by 1 nucleotides GFF3909 and GFF3910 overlap by 1 nucleotides
GFF3906 - Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) / Phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31), at 188,102 to 188,713
GFF3906
GFF3907 - Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-), at 188,707 to 189,483
GFF3907
GFF3908 - Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (EC 5.3.1.16), at 189,465 to 190,202
GFF3908
GFF3909 - Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-), at 190,202 to 190,792
GFF3909
GFF3910 - Histidinol-phosphatase (EC 3.1.3.15) / Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19), at 190,792 to 191,859
GFF3910
Position (kb) 189
190
191 Strain fitness (log2 ratio) -1
0
1
2 at 188.605 kb on + strand, within GFF3906 at 188.607 kb on + strand, within GFF3906 at 188.608 kb on - strand, within GFF3906 at 188.621 kb on + strand, within GFF3906 at 189.111 kb on + strand, within GFF3907 at 189.111 kb on + strand, within GFF3907 at 189.111 kb on + strand, within GFF3907 at 189.112 kb on - strand, within GFF3907 at 189.467 kb on - strand at 189.700 kb on - strand, within GFF3908 at 189.700 kb on - strand, within GFF3908 at 189.700 kb on - strand, within GFF3908 at 189.808 kb on - strand, within GFF3908 at 190.186 kb on - strand at 190.203 kb on + strand at 190.268 kb on + strand, within GFF3909 at 190.269 kb on - strand, within GFF3909 at 190.586 kb on - strand, within GFF3909 at 190.593 kb on + strand, within GFF3909 at 190.595 kb on - strand, within GFF3909 at 190.754 kb on + strand at 191.005 kb on - strand, within GFF3910 at 191.016 kb on + strand, within GFF3910 at 191.164 kb on + strand, within GFF3910 at 191.187 kb on - strand, within GFF3910 at 191.187 kb on - strand, within GFF3910 at 191.187 kb on - strand, within GFF3910
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 188,605 + GFF3906 0.82 -0.7 188,607 + GFF3906 0.83 -0.4 188,608 - GFF3906 0.83 +0.3 188,621 + GFF3906 0.85 -1.5 189,111 + GFF3907 0.52 -0.4 189,111 + GFF3907 0.52 -1.5 189,111 + GFF3907 0.52 -1.5 189,112 - GFF3907 0.52 -0.7 189,467 - -0.5 189,700 - GFF3908 0.32 +2.5 189,700 - GFF3908 0.32 -1.1 189,700 - GFF3908 0.32 -1.0 189,808 - GFF3908 0.46 +0.7 190,186 - +1.1 190,203 + -0.0 190,268 + GFF3909 0.11 -0.2 190,269 - GFF3909 0.11 +0.0 190,586 - GFF3909 0.65 -0.3 190,593 + GFF3909 0.66 +0.3 190,595 - GFF3909 0.66 +0.8 190,754 + +0.1 191,005 - GFF3910 0.20 -1.1 191,016 + GFF3910 0.21 -1.7 191,164 + GFF3910 0.35 +0.0 191,187 - GFF3910 0.37 +0.3 191,187 - GFF3910 0.37 +0.0 191,187 - GFF3910 0.37 -1.5
Or see this region's nucleotide sequence