Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3852 and GFF3853 overlap by 4 nucleotides GFF3853 and GFF3854 are separated by 0 nucleotides
GFF3852 - Multidrug transporter MdtD, at 121,715 to 123,127
GFF3852
GFF3853 - Multidrug transporter MdtC, at 123,124 to 126,204
GFF3853
GFF3854 - Multidrug transporter MdtB, at 126,205 to 129,327
GFF3854
Position (kb) 123
124
125
126
127 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 122.168 kb on + strand, within GFF3852 at 122.169 kb on - strand, within GFF3852 at 122.558 kb on + strand, within GFF3852 at 122.559 kb on - strand, within GFF3852 at 122.566 kb on - strand, within GFF3852 at 122.593 kb on + strand, within GFF3852 at 122.731 kb on + strand, within GFF3852 at 122.733 kb on + strand, within GFF3852 at 122.733 kb on + strand, within GFF3852 at 122.734 kb on - strand, within GFF3852 at 122.734 kb on - strand, within GFF3852 at 122.820 kb on - strand, within GFF3852 at 122.899 kb on + strand, within GFF3852 at 122.967 kb on + strand, within GFF3852 at 122.968 kb on - strand, within GFF3852 at 122.968 kb on - strand, within GFF3852 at 122.968 kb on - strand, within GFF3852 at 123.133 kb on + strand at 123.296 kb on - strand at 123.439 kb on - strand, within GFF3853 at 123.493 kb on + strand, within GFF3853 at 123.615 kb on - strand, within GFF3853 at 123.866 kb on + strand, within GFF3853 at 123.867 kb on - strand, within GFF3853 at 123.966 kb on + strand, within GFF3853 at 123.966 kb on + strand, within GFF3853 at 123.966 kb on + strand, within GFF3853 at 124.082 kb on + strand, within GFF3853 at 124.086 kb on + strand, within GFF3853 at 124.087 kb on - strand, within GFF3853 at 124.215 kb on + strand, within GFF3853 at 124.216 kb on - strand, within GFF3853 at 124.216 kb on - strand, within GFF3853 at 124.575 kb on - strand, within GFF3853 at 124.629 kb on + strand, within GFF3853 at 124.636 kb on - strand, within GFF3853 at 124.734 kb on + strand, within GFF3853 at 124.923 kb on + strand, within GFF3853 at 125.072 kb on + strand, within GFF3853 at 125.073 kb on - strand, within GFF3853 at 125.125 kb on + strand, within GFF3853 at 125.152 kb on + strand, within GFF3853 at 125.152 kb on + strand, within GFF3853 at 125.188 kb on - strand, within GFF3853 at 125.203 kb on + strand, within GFF3853 at 125.203 kb on + strand, within GFF3853 at 125.203 kb on + strand, within GFF3853 at 125.444 kb on - strand, within GFF3853 at 125.460 kb on + strand, within GFF3853 at 125.460 kb on + strand, within GFF3853 at 125.461 kb on - strand, within GFF3853 at 125.462 kb on + strand, within GFF3853 at 125.463 kb on - strand, within GFF3853 at 125.538 kb on + strand, within GFF3853 at 125.538 kb on + strand, within GFF3853 at 125.539 kb on - strand, within GFF3853 at 125.772 kb on + strand, within GFF3853 at 125.773 kb on - strand, within GFF3853 at 125.787 kb on + strand, within GFF3853 at 125.901 kb on + strand at 125.905 kb on - strand at 125.905 kb on - strand at 126.206 kb on + strand at 126.207 kb on - strand at 126.302 kb on - strand at 126.459 kb on + strand at 126.675 kb on - strand, within GFF3854 at 126.695 kb on + strand, within GFF3854 at 126.696 kb on - strand, within GFF3854 at 126.763 kb on - strand, within GFF3854 at 126.763 kb on - strand, within GFF3854 at 126.898 kb on + strand, within GFF3854 at 127.030 kb on - strand, within GFF3854
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 122,168 + GFF3852 0.32 -0.7 122,169 - GFF3852 0.32 +0.9 122,558 + GFF3852 0.60 -0.9 122,559 - GFF3852 0.60 +0.1 122,566 - GFF3852 0.60 -0.0 122,593 + GFF3852 0.62 +0.6 122,731 + GFF3852 0.72 +0.5 122,733 + GFF3852 0.72 -0.0 122,733 + GFF3852 0.72 +0.2 122,734 - GFF3852 0.72 +1.2 122,734 - GFF3852 0.72 +0.6 122,820 - GFF3852 0.78 +0.9 122,899 + GFF3852 0.84 -0.0 122,967 + GFF3852 0.89 +4.3 122,968 - GFF3852 0.89 +0.8 122,968 - GFF3852 0.89 -0.0 122,968 - GFF3852 0.89 +0.0 123,133 + +3.2 123,296 - +3.5 123,439 - GFF3853 0.10 +0.5 123,493 + GFF3853 0.12 -0.4 123,615 - GFF3853 0.16 -0.4 123,866 + GFF3853 0.24 -1.1 123,867 - GFF3853 0.24 -0.0 123,966 + GFF3853 0.27 -0.7 123,966 + GFF3853 0.27 +0.3 123,966 + GFF3853 0.27 +0.3 124,082 + GFF3853 0.31 +1.0 124,086 + GFF3853 0.31 -0.8 124,087 - GFF3853 0.31 +2.0 124,215 + GFF3853 0.35 -0.6 124,216 - GFF3853 0.35 +2.1 124,216 - GFF3853 0.35 -1.2 124,575 - GFF3853 0.47 -0.6 124,629 + GFF3853 0.49 +0.7 124,636 - GFF3853 0.49 +0.4 124,734 + GFF3853 0.52 -0.5 124,923 + GFF3853 0.58 -0.5 125,072 + GFF3853 0.63 +0.7 125,073 - GFF3853 0.63 +0.4 125,125 + GFF3853 0.65 +0.6 125,152 + GFF3853 0.66 +0.2 125,152 + GFF3853 0.66 -0.0 125,188 - GFF3853 0.67 +0.1 125,203 + GFF3853 0.67 -0.2 125,203 + GFF3853 0.67 -0.9 125,203 + GFF3853 0.67 +0.1 125,444 - GFF3853 0.75 -0.3 125,460 + GFF3853 0.76 +0.6 125,460 + GFF3853 0.76 +0.6 125,461 - GFF3853 0.76 -1.0 125,462 + GFF3853 0.76 +0.2 125,463 - GFF3853 0.76 +1.2 125,538 + GFF3853 0.78 -0.1 125,538 + GFF3853 0.78 -1.1 125,539 - GFF3853 0.78 -0.7 125,772 + GFF3853 0.86 -0.9 125,773 - GFF3853 0.86 +0.2 125,787 + GFF3853 0.86 +0.0 125,901 + -0.6 125,905 - +0.2 125,905 - +0.0 126,206 + +3.3 126,207 - +0.3 126,302 - -0.8 126,459 + -0.6 126,675 - GFF3854 0.15 -0.4 126,695 + GFF3854 0.16 +0.0 126,696 - GFF3854 0.16 +0.2 126,763 - GFF3854 0.18 +0.3 126,763 - GFF3854 0.18 +0.7 126,898 + GFF3854 0.22 +1.8 127,030 - GFF3854 0.26 -0.3
Or see this region's nucleotide sequence