Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3774 and GFF3775 are separated by 78 nucleotides GFF3775 and GFF3776 overlap by 45 nucleotides GFF3776 and GFF3777 are separated by 27 nucleotides
GFF3774 - Endonuclease IV (EC 3.1.21.2), at 41,335 to 42,192
GFF3774
GFF3775 - Putative membrane protein YeiH, at 42,271 to 43,320
GFF3775
GFF3776 - hypothetical protein, at 43,276 to 43,413
GFF3776
GFF3777 - LysR family transcriptional regulator YeiE, at 43,441 to 44,304
GFF3777
Position (kb) 42
43
44 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 41.451 kb on + strand, within GFF3774 at 41.656 kb on - strand, within GFF3774 at 41.661 kb on + strand, within GFF3774 at 41.682 kb on + strand, within GFF3774 at 41.683 kb on - strand, within GFF3774 at 41.736 kb on + strand, within GFF3774 at 41.800 kb on - strand, within GFF3774 at 41.970 kb on + strand, within GFF3774 at 42.186 kb on - strand at 42.186 kb on - strand at 42.226 kb on + strand at 42.226 kb on + strand at 42.227 kb on - strand at 42.267 kb on + strand at 42.535 kb on - strand, within GFF3775 at 42.639 kb on + strand, within GFF3775 at 42.722 kb on + strand, within GFF3775 at 42.723 kb on - strand, within GFF3775 at 42.723 kb on - strand, within GFF3775 at 42.723 kb on - strand, within GFF3775 at 42.768 kb on - strand, within GFF3775 at 42.831 kb on + strand, within GFF3775 at 42.994 kb on + strand, within GFF3775 at 42.994 kb on + strand, within GFF3775 at 42.994 kb on + strand, within GFF3775 at 42.995 kb on - strand, within GFF3775 at 42.995 kb on - strand, within GFF3775 at 43.126 kb on + strand, within GFF3775 at 43.127 kb on - strand, within GFF3775 at 43.288 kb on - strand at 43.446 kb on + strand at 43.446 kb on + strand at 43.446 kb on + strand at 43.450 kb on - strand at 43.580 kb on + strand, within GFF3777 at 43.720 kb on + strand, within GFF3777 at 43.721 kb on - strand, within GFF3777 at 43.890 kb on + strand, within GFF3777 at 44.303 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 41,451 + GFF3774 0.14 -1.8 41,656 - GFF3774 0.37 -0.8 41,661 + GFF3774 0.38 -0.4 41,682 + GFF3774 0.40 -0.2 41,683 - GFF3774 0.41 -0.5 41,736 + GFF3774 0.47 -0.7 41,800 - GFF3774 0.54 +0.3 41,970 + GFF3774 0.74 -0.9 42,186 - +1.0 42,186 - +1.0 42,226 + +0.5 42,226 + -0.6 42,227 - +1.5 42,267 + +0.7 42,535 - GFF3775 0.25 -0.2 42,639 + GFF3775 0.35 +1.4 42,722 + GFF3775 0.43 +1.2 42,723 - GFF3775 0.43 -0.5 42,723 - GFF3775 0.43 +2.4 42,723 - GFF3775 0.43 -0.5 42,768 - GFF3775 0.47 -0.9 42,831 + GFF3775 0.53 +1.4 42,994 + GFF3775 0.69 +1.4 42,994 + GFF3775 0.69 -0.4 42,994 + GFF3775 0.69 -1.5 42,995 - GFF3775 0.69 -0.3 42,995 - GFF3775 0.69 +1.8 43,126 + GFF3775 0.81 +0.3 43,127 - GFF3775 0.82 -1.0 43,288 - +2.0 43,446 + +1.9 43,446 + +0.7 43,446 + +0.0 43,450 - -0.4 43,580 + GFF3777 0.16 -0.7 43,720 + GFF3777 0.32 -2.8 43,721 - GFF3777 0.32 -1.9 43,890 + GFF3777 0.52 +0.5 44,303 - +2.5
Or see this region's nucleotide sequence