Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3700

Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3699 and GFF3700 are separated by 334 nucleotidesGFF3700 and GFF3701 are separated by 11 nucleotidesGFF3701 and GFF3702 are separated by 109 nucleotides GFF3699 - putative surface-exposed virulence protein, at 78 to 5,765 GFF3699 GFF3700 - Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 6,100 to 7,473 GFF3700 GFF3701 - Nitrite transporter NirC, at 7,485 to 8,294 GFF3701 GFF3702 - hypothetical protein, at 8,404 to 8,559 GFF3702 Position (kb) 6 7 8Strain fitness (log2 ratio) -2 -1 0 1 2 3at 5.177 kb on + strand, within GFF3699at 5.178 kb on - strand, within GFF3699at 5.180 kb on + strand, within GFF3699at 5.236 kb on - strandat 5.236 kb on - strandat 5.236 kb on - strandat 5.236 kb on - strandat 5.318 kb on + strandat 5.489 kb on - strandat 5.489 kb on - strandat 5.498 kb on + strandat 5.499 kb on - strandat 5.601 kb on - strandat 5.601 kb on - strandat 6.416 kb on - strand, within GFF3700at 6.709 kb on - strand, within GFF3700at 7.140 kb on + strand, within GFF3700at 7.141 kb on - strand, within GFF3700at 7.321 kb on + strand, within GFF3700at 7.486 kb on + strandat 7.596 kb on - strand, within GFF3701at 7.644 kb on - strand, within GFF3701at 7.645 kb on + strand, within GFF3701at 7.646 kb on - strand, within GFF3701at 7.749 kb on - strand, within GFF3701at 7.927 kb on + strand, within GFF3701at 7.985 kb on + strand, within GFF3701at 8.127 kb on + strand, within GFF3701at 8.185 kb on + strand, within GFF3701at 8.186 kb on - strand, within GFF3701at 8.186 kb on - strand, within GFF3701at 8.273 kb on + strandat 8.366 kb on + strandat 8.366 kb on + strandat 8.374 kb on + strandat 8.374 kb on + strandat 8.375 kb on - strandat 8.375 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
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5,177 + GFF3699 0.90 +0.1
5,178 - GFF3699 0.90 +0.4
5,180 + GFF3699 0.90 -0.6
5,236 - -2.5
5,236 - +0.1
5,236 - +1.3
5,236 - -0.8
5,318 + +0.7
5,489 - +0.8
5,489 - -0.4
5,498 + -0.6
5,499 - +0.1
5,601 - +0.1
5,601 - +0.7
6,416 - GFF3700 0.23 -0.8
6,709 - GFF3700 0.44 -0.8
7,140 + GFF3700 0.76 +1.5
7,141 - GFF3700 0.76 -0.0
7,321 + GFF3700 0.89 -0.1
7,486 + +0.6
7,596 - GFF3701 0.14 +0.5
7,644 - GFF3701 0.20 +0.0
7,645 + GFF3701 0.20 +0.2
7,646 - GFF3701 0.20 +0.2
7,749 - GFF3701 0.33 +1.9
7,927 + GFF3701 0.55 +0.1
7,985 + GFF3701 0.62 -0.7
8,127 + GFF3701 0.79 +0.1
8,185 + GFF3701 0.86 -0.9
8,186 - GFF3701 0.87 +0.2
8,186 - GFF3701 0.87 -0.4
8,273 + -0.2
8,366 + -0.1
8,366 + -2.0
8,374 + +3.4
8,374 + +0.3
8,375 - -0.3
8,375 - +0.5

Or see this region's nucleotide sequence