Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3559 and GFF3560 are separated by 16 nucleotides GFF3560 and GFF3561 overlap by 26 nucleotides
GFF3559 - Iron-sulfur cluster assembly protein SufB, at 314,745 to 316,232
GFF3559
GFF3560 - Iron-sulfur cluster assembly ATPase protein SufC, at 316,249 to 316,995
GFF3560
GFF3561 - Iron-sulfur cluster assembly protein SufD, at 316,970 to 318,241
GFF3561
Position (kb) 316
317 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 315.291 kb on + strand, within GFF3559 at 315.368 kb on + strand, within GFF3559 at 315.368 kb on + strand, within GFF3559 at 315.368 kb on + strand, within GFF3559 at 315.368 kb on + strand, within GFF3559 at 315.369 kb on - strand, within GFF3559 at 315.369 kb on - strand, within GFF3559 at 315.373 kb on - strand, within GFF3559 at 315.425 kb on + strand, within GFF3559 at 315.484 kb on - strand, within GFF3559 at 315.484 kb on - strand, within GFF3559 at 315.500 kb on - strand, within GFF3559 at 315.529 kb on - strand, within GFF3559 at 315.529 kb on - strand, within GFF3559 at 315.567 kb on - strand, within GFF3559 at 316.022 kb on + strand, within GFF3559 at 316.022 kb on + strand, within GFF3559 at 316.260 kb on + strand at 316.440 kb on + strand, within GFF3560 at 316.658 kb on + strand, within GFF3560 at 317.067 kb on + strand at 317.266 kb on + strand, within GFF3561 at 317.384 kb on + strand, within GFF3561 at 317.384 kb on + strand, within GFF3561 at 317.384 kb on + strand, within GFF3561 at 317.384 kb on + strand, within GFF3561 at 317.385 kb on - strand, within GFF3561 at 317.388 kb on + strand, within GFF3561 at 317.388 kb on + strand, within GFF3561 at 317.388 kb on + strand, within GFF3561 at 317.389 kb on - strand, within GFF3561 at 317.431 kb on - strand, within GFF3561 at 317.437 kb on + strand, within GFF3561 at 317.546 kb on - strand, within GFF3561 at 317.546 kb on - strand, within GFF3561 at 317.727 kb on + strand, within GFF3561 at 317.728 kb on - strand, within GFF3561 at 317.782 kb on + strand, within GFF3561 at 317.783 kb on - strand, within GFF3561 at 317.839 kb on + strand, within GFF3561 at 317.839 kb on + strand, within GFF3561 at 317.854 kb on + strand, within GFF3561 at 317.854 kb on + strand, within GFF3561
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 315,291 + GFF3559 0.37 +0.5 315,368 + GFF3559 0.42 -0.1 315,368 + GFF3559 0.42 -1.5 315,368 + GFF3559 0.42 -0.8 315,368 + GFF3559 0.42 -0.9 315,369 - GFF3559 0.42 -0.7 315,369 - GFF3559 0.42 -1.1 315,373 - GFF3559 0.42 -0.2 315,425 + GFF3559 0.46 +0.5 315,484 - GFF3559 0.50 +0.5 315,484 - GFF3559 0.50 -0.9 315,500 - GFF3559 0.51 +0.1 315,529 - GFF3559 0.53 -0.4 315,529 - GFF3559 0.53 -0.1 315,567 - GFF3559 0.55 -0.1 316,022 + GFF3559 0.86 +0.8 316,022 + GFF3559 0.86 +0.8 316,260 + -0.1 316,440 + GFF3560 0.26 +3.9 316,658 + GFF3560 0.55 +1.3 317,067 + +0.4 317,266 + GFF3561 0.23 -0.4 317,384 + GFF3561 0.33 +0.5 317,384 + GFF3561 0.33 +0.2 317,384 + GFF3561 0.33 +0.4 317,384 + GFF3561 0.33 +2.6 317,385 - GFF3561 0.33 -0.3 317,388 + GFF3561 0.33 -0.2 317,388 + GFF3561 0.33 +0.1 317,388 + GFF3561 0.33 +2.1 317,389 - GFF3561 0.33 -1.4 317,431 - GFF3561 0.36 -0.8 317,437 + GFF3561 0.37 -0.4 317,546 - GFF3561 0.45 -0.4 317,546 - GFF3561 0.45 +0.4 317,727 + GFF3561 0.60 +0.5 317,728 - GFF3561 0.60 +0.3 317,782 + GFF3561 0.64 +0.4 317,783 - GFF3561 0.64 -0.8 317,839 + GFF3561 0.68 +0.3 317,839 + GFF3561 0.68 +1.9 317,854 + GFF3561 0.69 +1.0 317,854 + GFF3561 0.69 -0.1
Or see this region's nucleotide sequence