Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3276

Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3274 and GFF3275 are separated by 332 nucleotidesGFF3275 and GFF3276 are separated by 17 nucleotidesGFF3276 and GFF3277 are separated by 217 nucleotidesGFF3277 and GFF3278 are separated by 274 nucleotides GFF3274 - 5-Hydroxyisourate Hydrolase (HIUase) (EC 3.5.2.17), at 46,319 to 46,729 GFF3274 GFF3275 - 4-hydroxyphenylacetate 3-monooxygenase, reductase component (EC 1.6.8.-), at 47,062 to 47,574 GFF3275 GFF3276 - 4-hydroxyphenylacetate 3-monooxygenase (EC 1.14.13.3), at 47,592 to 49,154 GFF3276 GFF3277 - Homoprotocatechuate degradative operon repressor, at 49,372 to 49,812 GFF3277 GFF3278 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 50,087 to 51,376 GFF3278 Position (kb) 47 48 49 50Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 46.690 kb on - strandat 46.707 kb on - strandat 46.933 kb on + strandat 47.008 kb on - strandat 47.140 kb on - strand, within GFF3275at 47.149 kb on - strand, within GFF3275at 47.194 kb on + strand, within GFF3275at 47.235 kb on + strand, within GFF3275at 47.347 kb on + strand, within GFF3275at 47.349 kb on + strand, within GFF3275at 47.349 kb on + strand, within GFF3275at 47.350 kb on - strand, within GFF3275at 47.350 kb on - strand, within GFF3275at 47.353 kb on + strand, within GFF3275at 47.353 kb on + strand, within GFF3275at 47.358 kb on + strand, within GFF3275at 47.415 kb on + strand, within GFF3275at 47.415 kb on + strand, within GFF3275at 47.415 kb on + strand, within GFF3275at 47.416 kb on - strand, within GFF3275at 47.420 kb on - strand, within GFF3275at 47.630 kb on + strandat 47.706 kb on - strandat 47.712 kb on - strandat 47.838 kb on - strand, within GFF3276at 47.998 kb on + strand, within GFF3276at 48.089 kb on + strand, within GFF3276at 48.090 kb on - strand, within GFF3276at 48.253 kb on + strand, within GFF3276at 48.315 kb on + strand, within GFF3276at 48.316 kb on - strand, within GFF3276at 48.433 kb on + strand, within GFF3276at 48.547 kb on + strand, within GFF3276at 48.548 kb on - strand, within GFF3276at 48.597 kb on - strand, within GFF3276at 48.629 kb on + strand, within GFF3276at 48.631 kb on + strand, within GFF3276at 48.632 kb on - strand, within GFF3276at 48.686 kb on + strand, within GFF3276at 48.696 kb on - strand, within GFF3276at 48.700 kb on + strand, within GFF3276at 48.701 kb on - strand, within GFF3276at 48.701 kb on - strand, within GFF3276at 48.760 kb on + strand, within GFF3276at 48.918 kb on - strand, within GFF3276at 48.951 kb on + strand, within GFF3276at 48.952 kb on - strand, within GFF3276at 48.975 kb on - strand, within GFF3276at 49.235 kb on + strandat 49.370 kb on + strandat 49.371 kb on - strandat 49.371 kb on - strandat 49.374 kb on - strandat 49.374 kb on - strandat 49.416 kb on + strand, within GFF3277at 49.517 kb on + strand, within GFF3277at 49.517 kb on + strand, within GFF3277at 49.550 kb on + strand, within GFF3277at 49.550 kb on + strand, within GFF3277at 49.553 kb on - strand, within GFF3277at 49.553 kb on - strand, within GFF3277at 49.553 kb on - strand, within GFF3277at 49.553 kb on - strand, within GFF3277at 49.557 kb on + strand, within GFF3277at 49.557 kb on + strand, within GFF3277at 50.082 kb on + strandat 50.086 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
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46,690 - +0.7
46,707 - +0.4
46,933 + -2.1
47,008 - +0.2
47,140 - GFF3275 0.15 -0.2
47,149 - GFF3275 0.17 -0.7
47,194 + GFF3275 0.26 +2.9
47,235 + GFF3275 0.34 -0.5
47,347 + GFF3275 0.56 +1.0
47,349 + GFF3275 0.56 -0.6
47,349 + GFF3275 0.56 +0.0
47,350 - GFF3275 0.56 +1.8
47,350 - GFF3275 0.56 +0.2
47,353 + GFF3275 0.57 -0.2
47,353 + GFF3275 0.57 -0.2
47,358 + GFF3275 0.58 +0.5
47,415 + GFF3275 0.69 -0.5
47,415 + GFF3275 0.69 -0.4
47,415 + GFF3275 0.69 -0.3
47,416 - GFF3275 0.69 +0.5
47,420 - GFF3275 0.70 -0.4
47,630 + +0.5
47,706 - +0.7
47,712 - -0.5
47,838 - GFF3276 0.16 +1.0
47,998 + GFF3276 0.26 -1.2
48,089 + GFF3276 0.32 +0.2
48,090 - GFF3276 0.32 -0.0
48,253 + GFF3276 0.42 -0.4
48,315 + GFF3276 0.46 +1.3
48,316 - GFF3276 0.46 -0.1
48,433 + GFF3276 0.54 -0.1
48,547 + GFF3276 0.61 +0.4
48,548 - GFF3276 0.61 -0.4
48,597 - GFF3276 0.64 -0.5
48,629 + GFF3276 0.66 +0.1
48,631 + GFF3276 0.66 -0.0
48,632 - GFF3276 0.67 -0.9
48,686 + GFF3276 0.70 +2.1
48,696 - GFF3276 0.71 +0.8
48,700 + GFF3276 0.71 +0.5
48,701 - GFF3276 0.71 -1.2
48,701 - GFF3276 0.71 -1.4
48,760 + GFF3276 0.75 +0.8
48,918 - GFF3276 0.85 +0.3
48,951 + GFF3276 0.87 -0.9
48,952 - GFF3276 0.87 -0.9
48,975 - GFF3276 0.88 -0.1
49,235 + -0.9
49,370 + -0.6
49,371 - -1.7
49,371 - +0.4
49,374 - -0.2
49,374 - -1.7
49,416 + GFF3277 0.10 -0.4
49,517 + GFF3277 0.33 +1.2
49,517 + GFF3277 0.33 -1.1
49,550 + GFF3277 0.40 -0.3
49,550 + GFF3277 0.40 -1.7
49,553 - GFF3277 0.41 +0.7
49,553 - GFF3277 0.41 +4.2
49,553 - GFF3277 0.41 -1.0
49,553 - GFF3277 0.41 +0.8
49,557 + GFF3277 0.42 +0.1
49,557 + GFF3277 0.42 -0.0
50,082 + -0.1
50,086 + +1.4

Or see this region's nucleotide sequence