Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2845 and GFF2846 are separated by 19 nucleotides GFF2846 and GFF2847 are separated by 97 nucleotides GFF2847 and GFF2848 are separated by 128 nucleotides
GFF2845 - Tail-specific protease precursor (EC 3.4.21.102), at 366,406 to 368,454
GFF2845
GFF2846 - ProQ: influences osmotic activation of compatible solute ProP, at 368,474 to 369,160
GFF2846
GFF2847 - Free methionine-(R)-sulfoxide reductase, contains GAF domain, at 369,258 to 369,755
GFF2847
GFF2848 - Paraquat-inducible protein A, at 369,884 to 371,167
GFF2848
Position (kb)
368
369
370 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 367.672 kb on - strand, within GFF2845 at 367.681 kb on - strand, within GFF2845 at 367.682 kb on + strand, within GFF2845 at 367.744 kb on - strand, within GFF2845 at 367.772 kb on + strand, within GFF2845 at 368.215 kb on - strand, within GFF2845 at 368.217 kb on - strand, within GFF2845 at 368.284 kb on - strand at 368.376 kb on + strand at 368.377 kb on - strand at 368.377 kb on - strand at 368.380 kb on - strand at 368.380 kb on - strand at 368.440 kb on - strand at 368.559 kb on - strand, within GFF2846 at 368.720 kb on - strand, within GFF2846 at 368.939 kb on - strand, within GFF2846 at 368.946 kb on + strand, within GFF2846 at 368.947 kb on - strand, within GFF2846 at 368.968 kb on + strand, within GFF2846 at 368.971 kb on - strand, within GFF2846 at 368.979 kb on - strand, within GFF2846 at 368.979 kb on - strand, within GFF2846 at 369.137 kb on - strand at 369.146 kb on - strand at 369.174 kb on + strand at 369.220 kb on - strand at 369.221 kb on + strand at 369.222 kb on - strand at 369.236 kb on + strand at 369.244 kb on - strand at 369.244 kb on - strand at 369.380 kb on + strand, within GFF2847 at 369.385 kb on - strand, within GFF2847 at 369.385 kb on - strand, within GFF2847 at 369.464 kb on + strand, within GFF2847 at 369.494 kb on + strand, within GFF2847 at 369.614 kb on - strand, within GFF2847 at 369.654 kb on + strand, within GFF2847 at 369.726 kb on + strand at 369.737 kb on - strand at 369.737 kb on - strand at 369.739 kb on - strand at 369.757 kb on - strand at 369.757 kb on - strand at 369.777 kb on + strand at 369.798 kb on - strand at 369.809 kb on + strand at 369.853 kb on + strand at 369.858 kb on + strand at 370.031 kb on + strand, within GFF2848 at 370.032 kb on - strand, within GFF2848 at 370.116 kb on + strand, within GFF2848 at 370.153 kb on + strand, within GFF2848 at 370.157 kb on + strand, within GFF2848
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 367,672 - GFF2845 0.62 +1.8 367,681 - GFF2845 0.62 +1.7 367,682 + GFF2845 0.62 +1.3 367,744 - GFF2845 0.65 +0.8 367,772 + GFF2845 0.67 +0.9 368,215 - GFF2845 0.88 +0.9 368,217 - GFF2845 0.88 +1.2 368,284 - +0.0 368,376 + +1.3 368,377 - +2.4 368,377 - +0.8 368,380 - +2.3 368,380 - -0.7 368,440 - +0.9 368,559 - GFF2846 0.12 +0.8 368,720 - GFF2846 0.36 +1.4 368,939 - GFF2846 0.68 +1.5 368,946 + GFF2846 0.69 +4.7 368,947 - GFF2846 0.69 +0.3 368,968 + GFF2846 0.72 +1.9 368,971 - GFF2846 0.72 +0.3 368,979 - GFF2846 0.74 +0.4 368,979 - GFF2846 0.74 -0.6 369,137 - +0.2 369,146 - +0.2 369,174 + -0.2 369,220 - +1.7 369,221 + -1.2 369,222 - +1.0 369,236 + -1.1 369,244 - +0.1 369,244 - +0.1 369,380 + GFF2847 0.24 +0.5 369,385 - GFF2847 0.26 -0.7 369,385 - GFF2847 0.26 +0.5 369,464 + GFF2847 0.41 +0.2 369,494 + GFF2847 0.47 -0.8 369,614 - GFF2847 0.71 +1.1 369,654 + GFF2847 0.80 -0.0 369,726 + -0.3 369,737 - +0.6 369,737 - -0.1 369,739 - +0.3 369,757 - +1.6 369,757 - +1.6 369,777 + -2.1 369,798 - +1.3 369,809 + +0.4 369,853 + -0.1 369,858 + +0.1 370,031 + GFF2848 0.11 +0.6 370,032 - GFF2848 0.12 -0.6 370,116 + GFF2848 0.18 -2.6 370,153 + GFF2848 0.21 -0.2 370,157 + GFF2848 0.21 +0.4
Or see this region's nucleotide sequence