Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2826 and GFF2827 are separated by 3 nucleotides GFF2827 and GFF2828 are separated by 329 nucleotides GFF2828 and GFF2829 are separated by 137 nucleotides
GFF2826 - Para-aminobenzoate synthase, aminase component (EC 2.6.1.85), at 346,438 to 347,802
GFF2826
GFF2827 - Hypothetical nudix hydrolase YeaB, at 347,806 to 348,384
GFF2827
GFF2828 - L-serine dehydratase, beta subunit (EC 4.3.1.17) / L-serine dehydratase, alpha subunit (EC 4.3.1.17), at 348,714 to 350,012
GFF2828
GFF2829 - Rtn protein, at 350,150 to 351,751
GFF2829
Position (kb) 348
349
350
351 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 347.804 kb on - strand at 347.885 kb on + strand, within GFF2827 at 348.159 kb on + strand, within GFF2827 at 348.316 kb on + strand, within GFF2827 at 348.388 kb on + strand at 348.389 kb on - strand at 348.464 kb on - strand at 348.466 kb on - strand at 348.466 kb on - strand at 348.466 kb on - strand at 348.467 kb on + strand at 348.467 kb on + strand at 348.468 kb on - strand at 348.468 kb on - strand at 348.468 kb on - strand at 348.472 kb on - strand at 348.521 kb on - strand at 348.586 kb on - strand at 348.601 kb on - strand at 348.602 kb on + strand at 348.603 kb on - strand at 348.641 kb on + strand at 348.641 kb on + strand at 348.645 kb on - strand at 348.671 kb on + strand at 348.672 kb on - strand at 348.725 kb on + strand at 348.760 kb on + strand at 348.796 kb on - strand at 348.846 kb on - strand, within GFF2828 at 348.891 kb on - strand, within GFF2828 at 349.062 kb on + strand, within GFF2828 at 349.062 kb on + strand, within GFF2828 at 349.063 kb on - strand, within GFF2828 at 349.074 kb on + strand, within GFF2828 at 349.077 kb on + strand, within GFF2828 at 349.147 kb on + strand, within GFF2828 at 349.247 kb on + strand, within GFF2828 at 349.248 kb on - strand, within GFF2828 at 349.383 kb on - strand, within GFF2828 at 349.582 kb on - strand, within GFF2828 at 349.823 kb on + strand, within GFF2828 at 349.932 kb on + strand at 350.044 kb on - strand at 350.358 kb on + strand, within GFF2829 at 350.498 kb on - strand, within GFF2829 at 350.500 kb on + strand, within GFF2829 at 350.672 kb on - strand, within GFF2829 at 350.677 kb on - strand, within GFF2829 at 350.701 kb on + strand, within GFF2829 at 350.740 kb on - strand, within GFF2829 at 350.740 kb on - strand, within GFF2829 at 350.741 kb on + strand, within GFF2829 at 350.776 kb on - strand, within GFF2829 at 350.824 kb on + strand, within GFF2829 at 350.824 kb on + strand, within GFF2829 at 350.856 kb on + strand, within GFF2829 at 350.923 kb on + strand, within GFF2829 at 350.924 kb on - strand, within GFF2829
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 347,804 - +0.2 347,885 + GFF2827 0.14 +0.1 348,159 + GFF2827 0.61 +0.0 348,316 + GFF2827 0.88 +1.6 348,388 + +0.5 348,389 - -0.7 348,464 - -0.5 348,466 - +0.8 348,466 - -0.0 348,466 - -2.7 348,467 + +0.9 348,467 + +0.4 348,468 - -1.6 348,468 - -0.0 348,468 - -0.2 348,472 - +0.1 348,521 - -0.9 348,586 - -0.5 348,601 - +0.3 348,602 + +0.0 348,603 - -0.3 348,641 + -0.4 348,641 + -1.5 348,645 - -0.8 348,671 + -0.1 348,672 - +0.5 348,725 + -0.3 348,760 + -0.1 348,796 - -1.3 348,846 - GFF2828 0.10 +0.9 348,891 - GFF2828 0.14 -1.1 349,062 + GFF2828 0.27 +0.8 349,062 + GFF2828 0.27 +0.8 349,063 - GFF2828 0.27 +1.1 349,074 + GFF2828 0.28 +0.3 349,077 + GFF2828 0.28 +1.1 349,147 + GFF2828 0.33 +0.6 349,247 + GFF2828 0.41 +3.9 349,248 - GFF2828 0.41 -0.4 349,383 - GFF2828 0.52 +0.5 349,582 - GFF2828 0.67 +0.2 349,823 + GFF2828 0.85 +1.1 349,932 + +0.7 350,044 - +0.5 350,358 + GFF2829 0.13 +0.3 350,498 - GFF2829 0.22 +0.9 350,500 + GFF2829 0.22 +0.1 350,672 - GFF2829 0.33 -0.2 350,677 - GFF2829 0.33 -0.5 350,701 + GFF2829 0.34 -0.2 350,740 - GFF2829 0.37 +0.1 350,740 - GFF2829 0.37 -0.0 350,741 + GFF2829 0.37 +0.2 350,776 - GFF2829 0.39 -1.4 350,824 + GFF2829 0.42 -0.5 350,824 + GFF2829 0.42 -1.2 350,856 + GFF2829 0.44 +0.1 350,923 + GFF2829 0.48 +0.5 350,924 - GFF2829 0.48 -0.3
Or see this region's nucleotide sequence