Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2793 and GFF2794 are separated by 11 nucleotides GFF2794 and GFF2795 are separated by 496 nucleotides
GFF2793 - Cytochrome d ubiquinol oxidase subunit I (EC 1.10.3.-), at 315,662 to 317,206
GFF2793
GFF2794 - Cytochrome d ubiquinol oxidase subunit II (EC 1.10.3.-), at 317,218 to 318,354
GFF2794
GFF2795 - NAD-specific glutamate dehydrogenase (EC 1.4.1.2); NADP-specific glutamate dehydrogenase (EC 1.4.1.4), at 318,851 to 320,125
GFF2795
Position (kb) 317
318
319 Strain fitness (log2 ratio) -2
-1
0
1
2 at 316.235 kb on + strand, within GFF2793 at 316.835 kb on - strand, within GFF2793 at 316.837 kb on + strand, within GFF2793 at 316.838 kb on - strand, within GFF2793 at 316.863 kb on + strand, within GFF2793 at 316.888 kb on - strand, within GFF2793 at 316.988 kb on - strand, within GFF2793 at 317.147 kb on + strand at 317.228 kb on - strand at 317.452 kb on + strand, within GFF2794 at 317.708 kb on + strand, within GFF2794 at 317.727 kb on - strand, within GFF2794 at 317.760 kb on + strand, within GFF2794 at 317.994 kb on - strand, within GFF2794 at 318.171 kb on + strand, within GFF2794 at 318.274 kb on + strand at 318.275 kb on - strand at 318.276 kb on + strand at 318.463 kb on - strand at 318.557 kb on + strand at 318.796 kb on + strand at 318.803 kb on + strand at 318.826 kb on - strand at 318.866 kb on + strand at 318.898 kb on + strand at 318.898 kb on + strand at 319.023 kb on + strand, within GFF2795 at 319.253 kb on + strand, within GFF2795 at 319.274 kb on - strand, within GFF2795 at 319.294 kb on + strand, within GFF2795 at 319.324 kb on + strand, within GFF2795 at 319.352 kb on + strand, within GFF2795 at 319.352 kb on + strand, within GFF2795
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 316,235 + GFF2793 0.37 +0.5 316,835 - GFF2793 0.76 +1.0 316,837 + GFF2793 0.76 +2.4 316,838 - GFF2793 0.76 +1.2 316,863 + GFF2793 0.78 -0.5 316,888 - GFF2793 0.79 -1.5 316,988 - GFF2793 0.86 +1.2 317,147 + +0.3 317,228 - -0.5 317,452 + GFF2794 0.21 +0.7 317,708 + GFF2794 0.43 +1.0 317,727 - GFF2794 0.45 -0.7 317,760 + GFF2794 0.48 -0.2 317,994 - GFF2794 0.68 -0.9 318,171 + GFF2794 0.84 -1.9 318,274 + -0.1 318,275 - -0.7 318,276 + +0.5 318,463 - -0.0 318,557 + -2.1 318,796 + +0.5 318,803 + +0.8 318,826 - +0.2 318,866 + +0.0 318,898 + +0.8 318,898 + +0.3 319,023 + GFF2795 0.13 +0.3 319,253 + GFF2795 0.32 +0.8 319,274 - GFF2795 0.33 -1.2 319,294 + GFF2795 0.35 +0.5 319,324 + GFF2795 0.37 +0.5 319,352 + GFF2795 0.39 -0.5 319,352 + GFF2795 0.39 +0.0
Or see this region's nucleotide sequence