Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2699 and GFF2700 are separated by 121 nucleotides GFF2700 and GFF2701 are separated by 86 nucleotides GFF2701 and GFF2702 are separated by 258 nucleotides GFF2702 and GFF2703 are separated by 119 nucleotides GFF2703 and GFF2704 overlap by 1 nucleotides
GFF2699 - FIG00637999: hypothetical protein, at 222,151 to 224,142
GFF2699
GFF2700 - FIG01045186: hypothetical protein, at 224,264 to 224,443
GFF2700
GFF2701 - Transcriptional regulatory protein YciT, at 224,530 to 225,282
GFF2701
GFF2702 - Osmotically inducible lipoprotein B precursor, at 225,541 to 225,759
GFF2702
GFF2703 - Translation initiation factor SUI1-related protein, at 225,879 to 226,205
GFF2703
GFF2704 - 'Orotidine 5'-phosphate decarboxylase (EC 4.1.1.23)' transl_table=11, at 226,205 to 227,002
GFF2704
Position (kb) 224
225
226 Strain fitness (log2 ratio) -1
0
1
2 at 223.532 kb on - strand, within GFF2699 at 223.586 kb on + strand, within GFF2699 at 223.690 kb on - strand, within GFF2699 at 223.923 kb on + strand, within GFF2699 at 224.006 kb on + strand at 224.110 kb on + strand at 224.183 kb on + strand at 224.184 kb on - strand at 224.184 kb on - strand at 224.354 kb on - strand, within GFF2700 at 224.529 kb on + strand at 224.529 kb on + strand at 224.666 kb on - strand, within GFF2701 at 224.666 kb on - strand, within GFF2701 at 224.670 kb on - strand, within GFF2701 at 224.745 kb on - strand, within GFF2701 at 224.884 kb on + strand, within GFF2701 at 224.966 kb on + strand, within GFF2701 at 225.491 kb on + strand at 226.113 kb on - strand, within GFF2703 at 226.178 kb on - strand at 226.234 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 223,532 - GFF2699 0.69 +1.0 223,586 + GFF2699 0.72 -0.4 223,690 - GFF2699 0.77 -0.0 223,923 + GFF2699 0.89 +0.1 224,006 + +1.6 224,110 + -0.8 224,183 + -0.2 224,184 - +0.4 224,184 - +0.0 224,354 - GFF2700 0.50 -0.2 224,529 + -1.0 224,529 + +0.2 224,666 - GFF2701 0.18 +0.1 224,666 - GFF2701 0.18 -0.5 224,670 - GFF2701 0.19 -0.3 224,745 - GFF2701 0.29 -1.1 224,884 + GFF2701 0.47 +0.7 224,966 + GFF2701 0.58 -1.3 225,491 + +2.5 226,113 - GFF2703 0.72 -0.0 226,178 - -0.3 226,234 + -0.9
Or see this region's nucleotide sequence