Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2691 and GFF2692 are separated by 1 nucleotides GFF2692 and GFF2693 are separated by 15 nucleotides GFF2693 and GFF2694 overlap by 13 nucleotides GFF2694 and GFF2695 are separated by 465 nucleotides
GFF2691 - Peptide transport system ATP-binding protein SapD, at 212,784 to 213,776
GFF2691
GFF2692 - Peptide transport system ATP-binding protein SapF, at 213,778 to 214,584
GFF2692
GFF2693 - FIG01046389: hypothetical protein, at 214,600 to 214,725
GFF2693
GFF2694 - FIG01046389: hypothetical protein, at 214,713 to 215,309
GFF2694
GFF2695 - Putative inner membrane protein, at 215,775 to 216,752
GFF2695
Position (kb) 214
215
216 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 214.324 kb on - strand, within GFF2692 at 214.387 kb on + strand, within GFF2692 at 214.388 kb on - strand, within GFF2692 at 214.390 kb on - strand, within GFF2692 at 214.393 kb on + strand, within GFF2692 at 214.394 kb on - strand, within GFF2692 at 214.425 kb on + strand, within GFF2692 at 214.426 kb on - strand, within GFF2692 at 214.428 kb on + strand, within GFF2692 at 214.727 kb on + strand at 214.918 kb on - strand, within GFF2694 at 214.950 kb on + strand, within GFF2694 at 215.013 kb on + strand, within GFF2694 at 215.014 kb on - strand, within GFF2694 at 215.014 kb on - strand, within GFF2694 at 215.026 kb on - strand, within GFF2694 at 215.410 kb on + strand at 215.483 kb on + strand at 215.574 kb on + strand at 215.575 kb on - strand at 216.171 kb on - strand, within GFF2695
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 214,324 - GFF2692 0.68 -0.7 214,387 + GFF2692 0.75 +0.9 214,388 - GFF2692 0.76 +0.3 214,390 - GFF2692 0.76 -1.4 214,393 + GFF2692 0.76 -2.6 214,394 - GFF2692 0.76 +0.6 214,425 + GFF2692 0.80 -0.4 214,426 - GFF2692 0.80 -1.3 214,428 + GFF2692 0.81 -3.2 214,727 + +0.6 214,918 - GFF2694 0.34 +1.0 214,950 + GFF2694 0.40 +0.4 215,013 + GFF2694 0.50 +0.3 215,014 - GFF2694 0.50 +1.8 215,014 - GFF2694 0.50 +0.2 215,026 - GFF2694 0.52 -1.8 215,410 + +0.1 215,483 + -0.7 215,574 + -1.4 215,575 - -0.7 216,171 - GFF2695 0.40 +0.5
Or see this region's nucleotide sequence