Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2558 and GFF2559 overlap by 8 nucleotides GFF2559 and GFF2560 are separated by 13 nucleotides GFF2560 and GFF2561 are separated by 22 nucleotides GFF2561 and GFF2562 are separated by 159 nucleotides
GFF2558 - Formate dehydrogenase N gamma subunit (EC 1.2.1.2), at 74,512 to 75,168
GFF2558
GFF2559 - Formate dehydrogenase N beta subunit (EC 1.2.1.2), at 75,161 to 76,045
GFF2559
GFF2560 - Formate dehydrogenase N alpha subunit (EC 1.2.1.2) @ selenocysteine-containing, at 76,059 to 79,106
GFF2560
GFF2561 - hypothetical protein, at 79,129 to 79,281
GFF2561
GFF2562 - Permease of the drug/metabolite transporter (DMT) superfamily, at 79,441 to 80,265
GFF2562
Position (kb)
76
77
78
79
80 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 75.220 kb on - strand at 75.220 kb on - strand at 75.369 kb on + strand, within GFF2559 at 75.409 kb on + strand, within GFF2559 at 75.745 kb on + strand, within GFF2559 at 75.745 kb on + strand, within GFF2559 at 75.746 kb on - strand, within GFF2559 at 75.850 kb on - strand, within GFF2559 at 75.883 kb on + strand, within GFF2559 at 75.969 kb on + strand at 76.045 kb on + strand at 76.046 kb on - strand at 76.297 kb on + strand at 76.298 kb on - strand at 76.298 kb on - strand at 76.314 kb on - strand at 76.377 kb on - strand, within GFF2560 at 76.377 kb on - strand, within GFF2560 at 76.381 kb on - strand, within GFF2560 at 76.400 kb on + strand, within GFF2560 at 76.424 kb on - strand, within GFF2560 at 76.424 kb on - strand, within GFF2560 at 76.483 kb on + strand, within GFF2560 at 76.589 kb on + strand, within GFF2560 at 76.596 kb on - strand, within GFF2560 at 76.667 kb on + strand, within GFF2560 at 76.771 kb on + strand, within GFF2560 at 76.977 kb on - strand, within GFF2560 at 77.009 kb on - strand, within GFF2560 at 77.232 kb on - strand, within GFF2560 at 77.309 kb on + strand, within GFF2560 at 77.309 kb on + strand, within GFF2560 at 77.310 kb on - strand, within GFF2560 at 77.375 kb on - strand, within GFF2560 at 77.504 kb on - strand, within GFF2560 at 77.617 kb on + strand, within GFF2560 at 77.666 kb on - strand, within GFF2560 at 77.837 kb on + strand, within GFF2560 at 77.838 kb on - strand, within GFF2560 at 77.884 kb on + strand, within GFF2560 at 77.885 kb on - strand, within GFF2560 at 77.995 kb on + strand, within GFF2560 at 78.088 kb on + strand, within GFF2560 at 78.089 kb on - strand, within GFF2560 at 78.105 kb on - strand, within GFF2560 at 78.194 kb on - strand, within GFF2560 at 78.194 kb on - strand, within GFF2560 at 78.293 kb on - strand, within GFF2560 at 78.293 kb on - strand, within GFF2560 at 78.446 kb on + strand, within GFF2560 at 78.449 kb on + strand, within GFF2560 at 78.735 kb on - strand, within GFF2560 at 78.783 kb on + strand, within GFF2560 at 78.784 kb on - strand, within GFF2560 at 78.805 kb on + strand at 78.920 kb on + strand at 78.922 kb on + strand at 78.922 kb on + strand at 78.923 kb on - strand at 78.992 kb on + strand at 79.029 kb on - strand at 79.196 kb on + strand, within GFF2561 at 79.254 kb on - strand, within GFF2561 at 79.279 kb on + strand at 79.339 kb on + strand at 79.406 kb on + strand at 79.409 kb on + strand at 79.410 kb on - strand at 79.505 kb on - strand at 79.505 kb on - strand at 79.545 kb on - strand, within GFF2562 at 79.573 kb on + strand, within GFF2562 at 79.686 kb on - strand, within GFF2562 at 79.827 kb on + strand, within GFF2562 at 79.884 kb on + strand, within GFF2562 at 79.919 kb on - strand, within GFF2562 at 79.979 kb on + strand, within GFF2562 at 80.013 kb on - strand, within GFF2562 at 80.093 kb on + strand, within GFF2562
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 75,220 - +0.1 75,220 - +1.5 75,369 + GFF2559 0.24 +1.9 75,409 + GFF2559 0.28 +0.9 75,745 + GFF2559 0.66 -1.1 75,745 + GFF2559 0.66 -0.5 75,746 - GFF2559 0.66 -0.1 75,850 - GFF2559 0.78 -0.8 75,883 + GFF2559 0.82 -0.3 75,969 + -0.7 76,045 + -0.4 76,046 - +0.3 76,297 + -0.6 76,298 - -0.1 76,298 - -0.8 76,314 - +0.2 76,377 - GFF2560 0.10 -1.2 76,377 - GFF2560 0.10 +0.4 76,381 - GFF2560 0.11 +0.9 76,400 + GFF2560 0.11 +1.4 76,424 - GFF2560 0.12 +1.0 76,424 - GFF2560 0.12 +0.0 76,483 + GFF2560 0.14 +0.0 76,589 + GFF2560 0.17 -0.9 76,596 - GFF2560 0.18 -1.4 76,667 + GFF2560 0.20 -0.3 76,771 + GFF2560 0.23 -0.6 76,977 - GFF2560 0.30 -0.2 77,009 - GFF2560 0.31 +1.1 77,232 - GFF2560 0.38 -2.1 77,309 + GFF2560 0.41 -0.4 77,309 + GFF2560 0.41 -0.7 77,310 - GFF2560 0.41 +0.5 77,375 - GFF2560 0.43 -0.7 77,504 - GFF2560 0.47 +0.0 77,617 + GFF2560 0.51 +0.5 77,666 - GFF2560 0.53 +0.1 77,837 + GFF2560 0.58 +0.3 77,838 - GFF2560 0.58 +1.3 77,884 + GFF2560 0.60 -0.7 77,885 - GFF2560 0.60 +2.0 77,995 + GFF2560 0.64 -2.7 78,088 + GFF2560 0.67 -0.8 78,089 - GFF2560 0.67 -0.5 78,105 - GFF2560 0.67 +1.1 78,194 - GFF2560 0.70 +5.9 78,194 - GFF2560 0.70 +3.0 78,293 - GFF2560 0.73 +0.3 78,293 - GFF2560 0.73 -0.3 78,446 + GFF2560 0.78 -0.6 78,449 + GFF2560 0.78 +1.3 78,735 - GFF2560 0.88 +0.3 78,783 + GFF2560 0.89 -0.2 78,784 - GFF2560 0.89 +0.9 78,805 + -0.2 78,920 + +0.5 78,922 + +1.2 78,922 + -0.4 78,923 - -0.2 78,992 + -0.8 79,029 - +2.7 79,196 + GFF2561 0.44 +0.1 79,254 - GFF2561 0.82 +0.2 79,279 + -1.4 79,339 + -1.5 79,406 + +0.0 79,409 + +1.9 79,410 - +0.4 79,505 - -2.2 79,505 - -0.4 79,545 - GFF2562 0.13 +0.5 79,573 + GFF2562 0.16 -1.5 79,686 - GFF2562 0.30 -0.4 79,827 + GFF2562 0.47 +0.6 79,884 + GFF2562 0.54 -0.9 79,919 - GFF2562 0.58 +0.5 79,979 + GFF2562 0.65 +0.6 80,013 - GFF2562 0.69 +0.1 80,093 + GFF2562 0.79 -0.1
Or see this region's nucleotide sequence