Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2495 and GFF2496 are separated by 180 nucleotides GFF2496 and GFF2497 are separated by 129 nucleotides GFF2497 and GFF2498 are separated by 138 nucleotides
GFF2495 - FIG00638357: hypothetical protein, at 13,219 to 13,422
GFF2495
GFF2496 - Transcriptional regulator, GntR family, at 13,603 to 14,289
GFF2496
GFF2497 - 3-hydroxypropionate dehydrogenase (EC 1.1.1.298), at 14,419 to 15,165
GFF2497
GFF2498 - Dipeptidyl carboxypeptidase Dcp (EC 3.4.15.5), at 15,304 to 17,346
GFF2498
Position (kb) 14
15
16 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 13.486 kb on - strand at 13.487 kb on + strand at 13.731 kb on + strand, within GFF2496 at 13.785 kb on + strand, within GFF2496 at 13.785 kb on + strand, within GFF2496 at 13.926 kb on + strand, within GFF2496 at 13.926 kb on + strand, within GFF2496 at 13.926 kb on + strand, within GFF2496 at 13.926 kb on + strand, within GFF2496 at 13.927 kb on - strand, within GFF2496 at 13.958 kb on + strand, within GFF2496 at 14.070 kb on - strand, within GFF2496 at 14.070 kb on - strand, within GFF2496 at 14.070 kb on - strand, within GFF2496 at 14.121 kb on + strand, within GFF2496 at 14.347 kb on + strand at 14.374 kb on + strand at 14.413 kb on - strand at 14.420 kb on + strand at 14.557 kb on - strand, within GFF2497 at 14.727 kb on - strand, within GFF2497 at 15.090 kb on + strand, within GFF2497 at 15.152 kb on - strand at 15.195 kb on + strand at 15.195 kb on + strand at 15.199 kb on - strand at 15.203 kb on + strand at 15.228 kb on - strand at 15.333 kb on + strand at 15.333 kb on + strand at 15.616 kb on - strand, within GFF2498 at 15.887 kb on + strand, within GFF2498 at 16.141 kb on + strand, within GFF2498
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 13,486 - -0.0 13,487 + -0.4 13,731 + GFF2496 0.19 -2.0 13,785 + GFF2496 0.26 -0.5 13,785 + GFF2496 0.26 +0.9 13,926 + GFF2496 0.47 -1.1 13,926 + GFF2496 0.47 -0.6 13,926 + GFF2496 0.47 +0.0 13,926 + GFF2496 0.47 +1.2 13,927 - GFF2496 0.47 -0.2 13,958 + GFF2496 0.52 -2.0 14,070 - GFF2496 0.68 +1.2 14,070 - GFF2496 0.68 +0.0 14,070 - GFF2496 0.68 -0.4 14,121 + GFF2496 0.75 -1.1 14,347 + -1.2 14,374 + -0.7 14,413 - +0.5 14,420 + -0.1 14,557 - GFF2497 0.18 -0.5 14,727 - GFF2497 0.41 -0.5 15,090 + GFF2497 0.90 -0.3 15,152 - -1.2 15,195 + +0.8 15,195 + +4.8 15,199 - -0.3 15,203 + +0.2 15,228 - +3.3 15,333 + -0.6 15,333 + +2.8 15,616 - GFF2498 0.15 +0.4 15,887 + GFF2498 0.29 -1.2 16,141 + GFF2498 0.41 +0.2
Or see this region's nucleotide sequence