Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2453 and GFF2454 are separated by 48 nucleotides GFF2454 and GFF2455 are separated by 76 nucleotides GFF2455 and GFF2456 overlap by 4 nucleotides GFF2456 and GFF2457 are separated by 79 nucleotides
GFF2453 - Di/tripeptide permease YjdL, at 70,635 to 72,095
GFF2453
GFF2454 - Nitrogen regulatory protein P-II, at 72,144 to 72,482
GFF2454
GFF2455 - Putative sensory histidine kinase YfhA, at 72,559 to 73,896
GFF2455
GFF2456 - putative alpha helix protein, at 73,893 to 74,579
GFF2456
GFF2457 - Putative sensor-like histidine kinase YfhK, at 74,659 to 76,044
GFF2457
Position (kb) 72
73
74 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 71.863 kb on + strand, within GFF2453 at 71.873 kb on + strand, within GFF2453 at 71.877 kb on + strand, within GFF2453 at 71.877 kb on + strand, within GFF2453 at 71.877 kb on + strand, within GFF2453 at 71.878 kb on - strand, within GFF2453 at 71.880 kb on - strand, within GFF2453 at 71.881 kb on - strand, within GFF2453 at 72.329 kb on + strand, within GFF2454 at 72.330 kb on - strand, within GFF2454 at 72.331 kb on + strand, within GFF2454 at 72.410 kb on + strand, within GFF2454 at 72.410 kb on + strand, within GFF2454 at 72.411 kb on - strand, within GFF2454 at 72.546 kb on + strand at 72.601 kb on + strand at 72.601 kb on + strand at 72.602 kb on - strand at 72.657 kb on + strand at 72.658 kb on - strand at 72.690 kb on - strand at 72.690 kb on - strand at 73.010 kb on + strand, within GFF2455 at 73.594 kb on + strand, within GFF2455 at 73.702 kb on - strand, within GFF2455 at 73.760 kb on - strand, within GFF2455 at 73.760 kb on - strand, within GFF2455 at 73.762 kb on + strand, within GFF2455 at 73.985 kb on - strand, within GFF2456 at 73.985 kb on - strand, within GFF2456 at 74.054 kb on + strand, within GFF2456 at 74.135 kb on + strand, within GFF2456 at 74.136 kb on - strand, within GFF2456 at 74.221 kb on - strand, within GFF2456 at 74.221 kb on - strand, within GFF2456 at 74.327 kb on + strand, within GFF2456 at 74.327 kb on + strand, within GFF2456 at 74.488 kb on + strand, within GFF2456 at 74.572 kb on - strand at 74.660 kb on + strand at 74.661 kb on - strand at 74.757 kb on + strand at 74.883 kb on + strand, within GFF2457
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 71,863 + GFF2453 0.84 +0.7 71,873 + GFF2453 0.85 -0.5 71,877 + GFF2453 0.85 -0.6 71,877 + GFF2453 0.85 +1.5 71,877 + GFF2453 0.85 +1.5 71,878 - GFF2453 0.85 +0.2 71,880 - GFF2453 0.85 -0.3 71,881 - GFF2453 0.85 +0.0 72,329 + GFF2454 0.55 -0.9 72,330 - GFF2454 0.55 +0.8 72,331 + GFF2454 0.55 +0.5 72,410 + GFF2454 0.78 -1.0 72,410 + GFF2454 0.78 +0.8 72,411 - GFF2454 0.79 -0.5 72,546 + -0.5 72,601 + +3.0 72,601 + -2.5 72,602 - -0.4 72,657 + +0.1 72,658 - +1.1 72,690 - +0.3 72,690 - +1.3 73,010 + GFF2455 0.34 +1.5 73,594 + GFF2455 0.77 -0.9 73,702 - GFF2455 0.85 +0.1 73,760 - GFF2455 0.90 +3.7 73,760 - GFF2455 0.90 -0.1 73,762 + GFF2455 0.90 -0.4 73,985 - GFF2456 0.13 +1.2 73,985 - GFF2456 0.13 +1.3 74,054 + GFF2456 0.23 -0.1 74,135 + GFF2456 0.35 +0.3 74,136 - GFF2456 0.35 +0.2 74,221 - GFF2456 0.48 +0.2 74,221 - GFF2456 0.48 +0.8 74,327 + GFF2456 0.63 -0.4 74,327 + GFF2456 0.63 -1.2 74,488 + GFF2456 0.87 -0.3 74,572 - -0.7 74,660 + -0.0 74,661 - +0.0 74,757 + +0.1 74,883 + GFF2457 0.16 +2.9
Or see this region's nucleotide sequence