Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2243 and GFF2244 are separated by 14 nucleotides GFF2244 and GFF2245 are separated by 14 nucleotides GFF2245 and GFF2246 are separated by 85 nucleotides
GFF2243 - GNAT family acetyltransferase YiiD potentially involved in tRNA processing, at 37,617 to 38,558
GFF2243
GFF2244 - FIG074102: hypothetical protein, at 38,573 to 39,001
GFF2244
GFF2245 - Sea40, at 39,016 to 39,327
GFF2245
GFF2246 - Lipase, GDXG family, at 39,413 to 40,342
GFF2246
Position (kb) 38
39
40 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 37.611 kb on - strand at 37.611 kb on - strand at 37.620 kb on + strand at 37.780 kb on + strand, within GFF2243 at 37.801 kb on - strand, within GFF2243 at 37.802 kb on + strand, within GFF2243 at 37.803 kb on - strand, within GFF2243 at 37.835 kb on + strand, within GFF2243 at 37.835 kb on + strand, within GFF2243 at 37.836 kb on - strand, within GFF2243 at 37.843 kb on + strand, within GFF2243 at 37.843 kb on + strand, within GFF2243 at 37.844 kb on - strand, within GFF2243 at 38.000 kb on + strand, within GFF2243 at 38.000 kb on + strand, within GFF2243 at 38.001 kb on - strand, within GFF2243 at 38.003 kb on + strand, within GFF2243 at 38.003 kb on + strand, within GFF2243 at 38.043 kb on - strand, within GFF2243 at 38.050 kb on + strand, within GFF2243 at 38.346 kb on - strand, within GFF2243 at 38.507 kb on - strand at 38.567 kb on - strand at 38.598 kb on - strand at 38.614 kb on + strand at 38.626 kb on + strand, within GFF2244 at 38.658 kb on - strand, within GFF2244 at 38.658 kb on - strand, within GFF2244 at 38.847 kb on - strand, within GFF2244 at 38.969 kb on - strand at 39.036 kb on - strand at 39.038 kb on + strand at 39.038 kb on + strand at 39.038 kb on + strand at 39.075 kb on + strand, within GFF2245 at 39.094 kb on - strand, within GFF2245 at 39.170 kb on + strand, within GFF2245 at 39.195 kb on + strand, within GFF2245 at 39.281 kb on + strand, within GFF2245 at 39.440 kb on + strand at 39.440 kb on + strand at 39.441 kb on - strand at 39.441 kb on - strand at 39.441 kb on - strand at 39.616 kb on + strand, within GFF2246 at 39.617 kb on - strand, within GFF2246 at 39.700 kb on + strand, within GFF2246 at 39.702 kb on + strand, within GFF2246 at 39.702 kb on + strand, within GFF2246 at 39.702 kb on + strand, within GFF2246 at 39.702 kb on + strand, within GFF2246 at 39.702 kb on + strand, within GFF2246 at 39.702 kb on + strand, within GFF2246 at 39.703 kb on - strand, within GFF2246 at 39.744 kb on + strand, within GFF2246 at 39.744 kb on + strand, within GFF2246 at 39.745 kb on - strand, within GFF2246 at 39.765 kb on + strand, within GFF2246 at 39.766 kb on - strand, within GFF2246 at 39.766 kb on - strand, within GFF2246 at 39.802 kb on - strand, within GFF2246 at 39.802 kb on - strand, within GFF2246 at 39.946 kb on + strand, within GFF2246 at 39.950 kb on - strand, within GFF2246 at 39.974 kb on - strand, within GFF2246
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 37,611 - +0.2 37,611 - -1.2 37,620 + +1.2 37,780 + GFF2243 0.17 +1.3 37,801 - GFF2243 0.20 -0.0 37,802 + GFF2243 0.20 -0.7 37,803 - GFF2243 0.20 -0.9 37,835 + GFF2243 0.23 -1.3 37,835 + GFF2243 0.23 +0.4 37,836 - GFF2243 0.23 -0.4 37,843 + GFF2243 0.24 +0.2 37,843 + GFF2243 0.24 -1.0 37,844 - GFF2243 0.24 +0.8 38,000 + GFF2243 0.41 -0.0 38,000 + GFF2243 0.41 -0.1 38,001 - GFF2243 0.41 +0.0 38,003 + GFF2243 0.41 +0.3 38,003 + GFF2243 0.41 +0.2 38,043 - GFF2243 0.45 -0.8 38,050 + GFF2243 0.46 -0.9 38,346 - GFF2243 0.77 +0.4 38,507 - +0.0 38,567 - +2.6 38,598 - -1.1 38,614 + -1.0 38,626 + GFF2244 0.12 -1.1 38,658 - GFF2244 0.20 +0.4 38,658 - GFF2244 0.20 +1.4 38,847 - GFF2244 0.64 -1.6 38,969 - -0.0 39,036 - +1.0 39,038 + -0.1 39,038 + -1.5 39,038 + +0.5 39,075 + GFF2245 0.19 -0.6 39,094 - GFF2245 0.25 +4.2 39,170 + GFF2245 0.49 +0.2 39,195 + GFF2245 0.57 +0.2 39,281 + GFF2245 0.85 -0.8 39,440 + -0.5 39,440 + -0.8 39,441 - -0.2 39,441 - -0.7 39,441 - -0.7 39,616 + GFF2246 0.22 +0.4 39,617 - GFF2246 0.22 +0.7 39,700 + GFF2246 0.31 +0.9 39,702 + GFF2246 0.31 -0.5 39,702 + GFF2246 0.31 +0.6 39,702 + GFF2246 0.31 +1.5 39,702 + GFF2246 0.31 -1.0 39,702 + GFF2246 0.31 -0.0 39,702 + GFF2246 0.31 +0.5 39,703 - GFF2246 0.31 -0.9 39,744 + GFF2246 0.36 +0.1 39,744 + GFF2246 0.36 +1.2 39,745 - GFF2246 0.36 -0.8 39,765 + GFF2246 0.38 +2.7 39,766 - GFF2246 0.38 +0.9 39,766 - GFF2246 0.38 -0.3 39,802 - GFF2246 0.42 -0.7 39,802 - GFF2246 0.42 +1.5 39,946 + GFF2246 0.57 +0.0 39,950 - GFF2246 0.58 -0.9 39,974 - GFF2246 0.60 -1.0
Or see this region's nucleotide sequence