Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF223 and GFF224 are separated by 158 nucleotides GFF224 and GFF225 are separated by 16 nucleotides
GFF223 - Na(+)Citrate OH(-) antiporter, at 220,698 to 222,038
GFF223
GFF224 - Oxaloacetate decarboxylase gamma chain (EC 4.1.1.3), at 222,197 to 222,436
GFF224
GFF225 - Oxaloacetate decarboxylase alpha chain (EC 4.1.1.3), at 222,453 to 224,228
GFF225
Position (kb) 222
223 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 221.339 kb on + strand, within GFF223 at 221.340 kb on - strand, within GFF223 at 221.395 kb on + strand, within GFF223 at 221.442 kb on - strand, within GFF223 at 221.544 kb on + strand, within GFF223 at 221.545 kb on - strand, within GFF223 at 221.704 kb on + strand, within GFF223 at 221.722 kb on + strand, within GFF223 at 221.722 kb on + strand, within GFF223 at 221.722 kb on + strand, within GFF223 at 221.723 kb on - strand, within GFF223 at 221.740 kb on - strand, within GFF223 at 221.790 kb on - strand, within GFF223 at 221.945 kb on + strand at 221.945 kb on + strand at 221.945 kb on + strand at 221.961 kb on + strand at 221.962 kb on - strand at 221.962 kb on - strand at 222.036 kb on + strand at 222.085 kb on - strand at 222.210 kb on + strand at 222.210 kb on + strand at 222.211 kb on - strand at 222.213 kb on + strand at 222.213 kb on + strand at 222.214 kb on - strand at 222.434 kb on + strand at 222.727 kb on - strand, within GFF225 at 222.883 kb on - strand, within GFF225 at 222.974 kb on + strand, within GFF225 at 222.974 kb on + strand, within GFF225 at 222.974 kb on + strand, within GFF225 at 222.975 kb on - strand, within GFF225 at 223.005 kb on + strand, within GFF225 at 223.159 kb on - strand, within GFF225 at 223.159 kb on - strand, within GFF225 at 223.279 kb on - strand, within GFF225
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 221,339 + GFF223 0.48 -0.7 221,340 - GFF223 0.48 -0.6 221,395 + GFF223 0.52 +0.9 221,442 - GFF223 0.55 -1.6 221,544 + GFF223 0.63 -0.0 221,545 - GFF223 0.63 +3.6 221,704 + GFF223 0.75 +2.0 221,722 + GFF223 0.76 -2.1 221,722 + GFF223 0.76 -0.2 221,722 + GFF223 0.76 -0.1 221,723 - GFF223 0.76 -1.1 221,740 - GFF223 0.78 -0.6 221,790 - GFF223 0.81 -0.4 221,945 + -1.7 221,945 + -1.5 221,945 + +1.9 221,961 + -0.2 221,962 - -0.8 221,962 - +1.1 222,036 + -1.3 222,085 - +0.0 222,210 + -0.2 222,210 + -0.4 222,211 - -0.4 222,213 + -0.3 222,213 + +0.4 222,214 - -0.5 222,434 + -0.2 222,727 - GFF225 0.15 +0.5 222,883 - GFF225 0.24 +0.1 222,974 + GFF225 0.29 +0.3 222,974 + GFF225 0.29 +0.0 222,974 + GFF225 0.29 -1.7 222,975 - GFF225 0.29 -1.6 223,005 + GFF225 0.31 +0.0 223,159 - GFF225 0.40 +1.1 223,159 - GFF225 0.40 +0.7 223,279 - GFF225 0.47 +4.7
Or see this region's nucleotide sequence