Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2233 and GFF2234 are separated by 43 nucleotides GFF2234 and GFF2235 are separated by 18 nucleotides
GFF2233 - Alpha-glucosyltransferase YihQ, at 27,589 to 29,625
GFF2233
GFF2234 - Sugar-1-epimerase YihR, at 29,669 to 30,511
GFF2234
GFF2235 - Aldose-ketose isomerase YihS, at 30,530 to 31,771
GFF2235
Position (kb)
29
30
31 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 28.689 kb on - strand, within GFF2233 at 28.773 kb on + strand, within GFF2233 at 28.897 kb on + strand, within GFF2233 at 28.898 kb on - strand, within GFF2233 at 28.932 kb on - strand, within GFF2233 at 28.932 kb on - strand, within GFF2233 at 28.955 kb on - strand, within GFF2233 at 28.955 kb on - strand, within GFF2233 at 28.965 kb on + strand, within GFF2233 at 29.021 kb on + strand, within GFF2233 at 29.022 kb on - strand, within GFF2233 at 29.028 kb on + strand, within GFF2233 at 29.028 kb on + strand, within GFF2233 at 29.029 kb on - strand, within GFF2233 at 29.029 kb on - strand, within GFF2233 at 29.046 kb on - strand, within GFF2233 at 29.048 kb on + strand, within GFF2233 at 29.049 kb on - strand, within GFF2233 at 29.094 kb on - strand, within GFF2233 at 29.119 kb on - strand, within GFF2233 at 29.214 kb on - strand, within GFF2233 at 29.238 kb on - strand, within GFF2233 at 29.238 kb on - strand, within GFF2233 at 29.238 kb on - strand, within GFF2233 at 29.241 kb on + strand, within GFF2233 at 29.242 kb on - strand, within GFF2233 at 29.269 kb on + strand, within GFF2233 at 29.277 kb on + strand, within GFF2233 at 29.443 kb on - strand at 29.460 kb on + strand at 29.461 kb on - strand at 29.743 kb on + strand at 29.812 kb on - strand, within GFF2234 at 29.819 kb on + strand, within GFF2234 at 29.872 kb on + strand, within GFF2234 at 29.900 kb on - strand, within GFF2234 at 29.911 kb on + strand, within GFF2234 at 30.002 kb on + strand, within GFF2234 at 30.010 kb on + strand, within GFF2234 at 30.169 kb on - strand, within GFF2234 at 30.216 kb on + strand, within GFF2234 at 30.221 kb on - strand, within GFF2234 at 30.309 kb on + strand, within GFF2234 at 30.310 kb on - strand, within GFF2234 at 30.315 kb on + strand, within GFF2234 at 30.315 kb on + strand, within GFF2234 at 30.531 kb on + strand at 30.531 kb on + strand at 30.627 kb on + strand at 30.627 kb on + strand at 30.632 kb on - strand at 30.632 kb on - strand at 30.708 kb on + strand, within GFF2235 at 30.724 kb on + strand, within GFF2235 at 30.724 kb on + strand, within GFF2235 at 30.725 kb on - strand, within GFF2235 at 30.725 kb on - strand, within GFF2235 at 30.843 kb on - strand, within GFF2235 at 30.848 kb on - strand, within GFF2235 at 30.853 kb on + strand, within GFF2235 at 31.072 kb on + strand, within GFF2235 at 31.073 kb on - strand, within GFF2235 at 31.081 kb on + strand, within GFF2235 at 31.082 kb on - strand, within GFF2235 at 31.218 kb on + strand, within GFF2235 at 31.249 kb on + strand, within GFF2235 at 31.338 kb on + strand, within GFF2235 at 31.356 kb on - strand, within GFF2235 at 31.460 kb on + strand, within GFF2235
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 28,689 - GFF2233 0.54 +0.5 28,773 + GFF2233 0.58 +2.6 28,897 + GFF2233 0.64 +0.4 28,898 - GFF2233 0.64 +0.2 28,932 - GFF2233 0.66 +0.4 28,932 - GFF2233 0.66 -1.0 28,955 - GFF2233 0.67 +0.6 28,955 - GFF2233 0.67 +3.7 28,965 + GFF2233 0.68 -0.7 29,021 + GFF2233 0.70 -1.8 29,022 - GFF2233 0.70 +2.7 29,028 + GFF2233 0.71 -0.5 29,028 + GFF2233 0.71 -0.7 29,029 - GFF2233 0.71 +0.6 29,029 - GFF2233 0.71 -0.1 29,046 - GFF2233 0.72 +1.6 29,048 + GFF2233 0.72 +0.0 29,049 - GFF2233 0.72 -1.0 29,094 - GFF2233 0.74 +0.4 29,119 - GFF2233 0.75 +2.3 29,214 - GFF2233 0.80 +0.7 29,238 - GFF2233 0.81 -1.3 29,238 - GFF2233 0.81 -1.8 29,238 - GFF2233 0.81 -0.5 29,241 + GFF2233 0.81 -1.3 29,242 - GFF2233 0.81 -0.2 29,269 + GFF2233 0.82 +1.5 29,277 + GFF2233 0.83 +0.2 29,443 - +0.5 29,460 + +0.4 29,461 - -0.5 29,743 + +0.0 29,812 - GFF2234 0.17 -1.1 29,819 + GFF2234 0.18 -0.5 29,872 + GFF2234 0.24 +0.2 29,900 - GFF2234 0.27 -0.7 29,911 + GFF2234 0.29 +2.2 30,002 + GFF2234 0.40 +5.3 30,010 + GFF2234 0.40 +0.0 30,169 - GFF2234 0.59 -0.9 30,216 + GFF2234 0.65 -0.3 30,221 - GFF2234 0.65 -0.0 30,309 + GFF2234 0.76 +0.7 30,310 - GFF2234 0.76 +0.9 30,315 + GFF2234 0.77 +1.1 30,315 + GFF2234 0.77 -1.7 30,531 + +2.4 30,531 + -2.3 30,627 + -0.3 30,627 + +0.7 30,632 - +2.5 30,632 - -0.7 30,708 + GFF2235 0.14 +0.6 30,724 + GFF2235 0.16 -0.9 30,724 + GFF2235 0.16 -1.3 30,725 - GFF2235 0.16 -0.6 30,725 - GFF2235 0.16 -1.1 30,843 - GFF2235 0.25 +1.1 30,848 - GFF2235 0.26 -0.7 30,853 + GFF2235 0.26 -2.1 31,072 + GFF2235 0.44 -0.1 31,073 - GFF2235 0.44 +0.2 31,081 + GFF2235 0.44 +2.6 31,082 - GFF2235 0.44 +0.1 31,218 + GFF2235 0.55 -1.9 31,249 + GFF2235 0.58 -1.4 31,338 + GFF2235 0.65 +0.5 31,356 - GFF2235 0.67 -0.5 31,460 + GFF2235 0.75 +0.4
Or see this region's nucleotide sequence