Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2156 and GFF2157 are separated by 37 nucleotides GFF2157 and GFF2114 are separated by 781 nucleotides GFF2114 and GFF2115 overlap by 25 nucleotides GFF2115 and GFF2116 are separated by 125 nucleotides
GFF2156 - tRNA-Thr-GGT, at 62 to 137
GFF2156
GFF2157 - 5S RNA, at 175 to 294
GFF2157
GFF2114 - probable lipoprotein, at 1,076 to 1,297
GFF2114
GFF2115 - FIG01045506: hypothetical protein, at 1,273 to 1,407
GFF2115
GFF2116 - RND efflux system, inner membrane transporter CmeB, at 1,533 to 4,646
GFF2116
Position (kb) 1
2 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 0.339 kb on + strand at 0.510 kb on - strand at 0.555 kb on + strand at 0.612 kb on + strand at 0.650 kb on - strand at 0.650 kb on - strand at 0.655 kb on + strand at 0.655 kb on + strand at 0.669 kb on - strand at 0.690 kb on + strand at 0.690 kb on + strand at 0.737 kb on - strand at 0.779 kb on + strand at 0.779 kb on + strand at 0.782 kb on + strand at 0.820 kb on + strand at 0.821 kb on - strand at 0.824 kb on + strand at 0.857 kb on - strand at 0.857 kb on - strand at 0.859 kb on - strand at 0.980 kb on - strand at 0.980 kb on - strand at 1.035 kb on - strand at 1.035 kb on - strand at 1.036 kb on + strand at 1.037 kb on - strand at 1.039 kb on + strand at 1.040 kb on - strand at 1.171 kb on + strand, within GFF2114 at 1.180 kb on + strand, within GFF2114 at 1.180 kb on + strand, within GFF2114 at 1.181 kb on - strand, within GFF2114 at 1.181 kb on - strand, within GFF2114 at 1.181 kb on - strand, within GFF2114 at 1.186 kb on + strand, within GFF2114 at 1.186 kb on + strand, within GFF2114 at 1.193 kb on - strand, within GFF2114 at 1.434 kb on + strand at 1.484 kb on + strand at 1.492 kb on + strand at 1.564 kb on - strand at 1.595 kb on + strand at 1.596 kb on - strand at 1.596 kb on - strand at 1.599 kb on + strand at 1.599 kb on + strand at 1.600 kb on - strand at 1.603 kb on - strand at 1.661 kb on + strand at 1.684 kb on - strand at 1.728 kb on - strand at 1.742 kb on + strand at 1.874 kb on - strand, within GFF2116 at 1.874 kb on - strand, within GFF2116 at 1.976 kb on - strand, within GFF2116 at 2.066 kb on + strand, within GFF2116 at 2.067 kb on - strand, within GFF2116 at 2.067 kb on - strand, within GFF2116 at 2.195 kb on - strand, within GFF2116 at 2.219 kb on - strand, within GFF2116 at 2.244 kb on - strand, within GFF2116 at 2.282 kb on - strand, within GFF2116
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 339 + +0.7 510 - +1.2 555 + -1.7 612 + -1.1 650 - -1.8 650 - +0.3 655 + -0.6 655 + +0.5 669 - +0.1 690 + +1.5 690 + -0.9 737 - +0.6 779 + +1.3 779 + +0.2 782 + -0.7 820 + -0.2 821 - +1.7 824 + +3.8 857 - +0.2 857 - -0.1 859 - +0.3 980 - +0.8 980 - -0.1 1,035 - -0.1 1,035 - +4.9 1,036 + -0.0 1,037 - +1.6 1,039 + -0.2 1,040 - -0.1 1,171 + GFF2114 0.43 +0.1 1,180 + GFF2114 0.47 +1.0 1,180 + GFF2114 0.47 -0.5 1,181 - GFF2114 0.47 -1.4 1,181 - GFF2114 0.47 -0.6 1,181 - GFF2114 0.47 -0.9 1,186 + GFF2114 0.50 -1.3 1,186 + GFF2114 0.50 -0.4 1,193 - GFF2114 0.53 -0.2 1,434 + +5.3 1,484 + +1.0 1,492 + -0.1 1,564 - +2.4 1,595 + +0.2 1,596 - +2.4 1,596 - -0.4 1,599 + +0.2 1,599 + +0.3 1,600 - -0.4 1,603 - -2.7 1,661 + -0.4 1,684 - +1.3 1,728 - -0.0 1,742 + -1.0 1,874 - GFF2116 0.11 -2.1 1,874 - GFF2116 0.11 +0.1 1,976 - GFF2116 0.14 +0.7 2,066 + GFF2116 0.17 +0.4 2,067 - GFF2116 0.17 +0.4 2,067 - GFF2116 0.17 +0.1 2,195 - GFF2116 0.21 -0.4 2,219 - GFF2116 0.22 +1.0 2,244 - GFF2116 0.23 +0.8 2,282 - GFF2116 0.24 +1.2
Or see this region's nucleotide sequence