Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1973 and GFF1974 are separated by 19 nucleotides GFF1974 and GFF1975 are separated by 179 nucleotides
GFF1973 - Chloride channel protein, at 165,523 to 166,758
GFF1973
GFF1974 - Pyruvate decarboxylase (EC 4.1.1.1); Alpha-keto-acid decarboxylase (EC 4.1.1.-), at 166,778 to 168,430
GFF1974
GFF1975 - Putative ion-channel protein, at 168,610 to 169,608
GFF1975
Position (kb) 166
167
168
169 Strain fitness (log2 ratio) -2
-1
0
1
2 at 165.842 kb on + strand, within GFF1973 at 165.867 kb on - strand, within GFF1973 at 165.900 kb on - strand, within GFF1973 at 166.320 kb on + strand, within GFF1973 at 166.322 kb on + strand, within GFF1973 at 166.428 kb on + strand, within GFF1973 at 166.429 kb on - strand, within GFF1973 at 166.970 kb on + strand, within GFF1974 at 166.971 kb on - strand, within GFF1974 at 167.002 kb on + strand, within GFF1974 at 167.003 kb on - strand, within GFF1974 at 167.037 kb on - strand, within GFF1974 at 167.041 kb on + strand, within GFF1974 at 167.044 kb on - strand, within GFF1974 at 167.228 kb on + strand, within GFF1974 at 167.322 kb on + strand, within GFF1974 at 167.330 kb on + strand, within GFF1974 at 167.446 kb on + strand, within GFF1974 at 167.590 kb on + strand, within GFF1974 at 167.609 kb on + strand, within GFF1974 at 167.610 kb on - strand, within GFF1974 at 167.649 kb on + strand, within GFF1974 at 167.649 kb on + strand, within GFF1974 at 167.702 kb on + strand, within GFF1974 at 167.940 kb on + strand, within GFF1974 at 167.941 kb on - strand, within GFF1974 at 168.011 kb on + strand, within GFF1974 at 168.012 kb on - strand, within GFF1974 at 168.184 kb on + strand, within GFF1974 at 168.219 kb on - strand, within GFF1974 at 168.416 kb on - strand at 168.454 kb on + strand at 168.455 kb on - strand at 168.457 kb on + strand at 168.457 kb on + strand at 168.526 kb on + strand at 168.542 kb on - strand at 168.576 kb on + strand at 168.577 kb on - strand at 168.806 kb on - strand, within GFF1975 at 169.015 kb on + strand, within GFF1975 at 169.068 kb on - strand, within GFF1975 at 169.099 kb on + strand, within GFF1975 at 169.114 kb on - strand, within GFF1975 at 169.117 kb on + strand, within GFF1975 at 169.143 kb on + strand, within GFF1975 at 169.192 kb on - strand, within GFF1975 at 169.195 kb on + strand, within GFF1975 at 169.306 kb on + strand, within GFF1975 at 169.307 kb on - strand, within GFF1975 at 169.374 kb on + strand, within GFF1975
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 165,842 + GFF1973 0.26 +1.3 165,867 - GFF1973 0.28 -0.3 165,900 - GFF1973 0.31 -0.1 166,320 + GFF1973 0.64 +0.5 166,322 + GFF1973 0.65 +0.3 166,428 + GFF1973 0.73 -2.3 166,429 - GFF1973 0.73 +0.2 166,970 + GFF1974 0.12 -1.3 166,971 - GFF1974 0.12 +0.1 167,002 + GFF1974 0.14 +1.9 167,003 - GFF1974 0.14 +0.8 167,037 - GFF1974 0.16 -0.3 167,041 + GFF1974 0.16 +1.1 167,044 - GFF1974 0.16 -0.5 167,228 + GFF1974 0.27 +0.4 167,322 + GFF1974 0.33 +0.7 167,330 + GFF1974 0.33 -0.2 167,446 + GFF1974 0.40 +1.1 167,590 + GFF1974 0.49 +0.2 167,609 + GFF1974 0.50 -0.4 167,610 - GFF1974 0.50 +1.7 167,649 + GFF1974 0.53 -0.6 167,649 + GFF1974 0.53 +0.5 167,702 + GFF1974 0.56 +2.6 167,940 + GFF1974 0.70 -0.7 167,941 - GFF1974 0.70 -0.4 168,011 + GFF1974 0.75 +2.1 168,012 - GFF1974 0.75 -1.9 168,184 + GFF1974 0.85 -0.1 168,219 - GFF1974 0.87 +0.8 168,416 - -1.3 168,454 + +1.8 168,455 - +0.6 168,457 + -0.5 168,457 + +0.1 168,526 + +0.2 168,542 - -0.6 168,576 + +0.8 168,577 - -0.1 168,806 - GFF1975 0.20 -0.3 169,015 + GFF1975 0.41 -0.5 169,068 - GFF1975 0.46 +0.1 169,099 + GFF1975 0.49 +1.4 169,114 - GFF1975 0.50 -0.1 169,117 + GFF1975 0.51 -0.3 169,143 + GFF1975 0.53 +0.5 169,192 - GFF1975 0.58 +0.5 169,195 + GFF1975 0.59 -1.2 169,306 + GFF1975 0.70 +0.3 169,307 - GFF1975 0.70 -1.0 169,374 + GFF1975 0.76 +0.4
Or see this region's nucleotide sequence