Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1957 and GFF1958 are separated by 60 nucleotides GFF1958 and GFF1959 are separated by 288 nucleotides
GFF1957 - Long-chain fatty acid transport protein, at 148,785 to 150,092
GFF1957
GFF1958 - Lipoprotein, at 150,153 to 150,908
GFF1958
GFF1959 - putative transport, at 151,197 to 152,138
GFF1959
Position (kb)
150
151 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 149.160 kb on - strand, within GFF1957 at 149.160 kb on - strand, within GFF1957 at 149.163 kb on + strand, within GFF1957 at 149.164 kb on - strand, within GFF1957 at 149.248 kb on + strand, within GFF1957 at 149.266 kb on + strand, within GFF1957 at 149.267 kb on - strand, within GFF1957 at 149.541 kb on + strand, within GFF1957 at 149.541 kb on + strand, within GFF1957 at 149.564 kb on + strand, within GFF1957 at 149.666 kb on + strand, within GFF1957 at 149.700 kb on - strand, within GFF1957 at 149.823 kb on + strand, within GFF1957 at 149.823 kb on + strand, within GFF1957 at 149.823 kb on + strand, within GFF1957 at 149.957 kb on + strand, within GFF1957 at 149.991 kb on + strand at 149.991 kb on + strand at 149.992 kb on - strand at 149.993 kb on + strand at 149.993 kb on + strand at 149.993 kb on + strand at 149.994 kb on - strand at 149.994 kb on - strand at 150.259 kb on + strand, within GFF1958 at 150.278 kb on + strand, within GFF1958 at 150.279 kb on - strand, within GFF1958 at 150.279 kb on - strand, within GFF1958 at 150.279 kb on - strand, within GFF1958 at 150.281 kb on - strand, within GFF1958 at 150.467 kb on + strand, within GFF1958 at 150.468 kb on - strand, within GFF1958 at 150.469 kb on + strand, within GFF1958 at 150.469 kb on + strand, within GFF1958 at 150.470 kb on - strand, within GFF1958 at 150.540 kb on - strand, within GFF1958 at 150.586 kb on - strand, within GFF1958 at 150.586 kb on - strand, within GFF1958 at 150.591 kb on - strand, within GFF1958 at 150.725 kb on - strand, within GFF1958 at 150.725 kb on - strand, within GFF1958 at 150.761 kb on - strand, within GFF1958 at 150.761 kb on - strand, within GFF1958 at 150.790 kb on + strand, within GFF1958 at 150.791 kb on - strand, within GFF1958 at 150.908 kb on + strand at 150.928 kb on - strand at 150.928 kb on - strand at 150.928 kb on - strand at 150.928 kb on - strand at 150.928 kb on - strand at 151.018 kb on + strand at 151.018 kb on + strand at 151.019 kb on - strand at 151.019 kb on - strand at 151.019 kb on - strand at 151.019 kb on - strand at 151.077 kb on - strand at 151.172 kb on + strand at 151.499 kb on + strand, within GFF1959 at 151.499 kb on + strand, within GFF1959 at 151.500 kb on - strand, within GFF1959 at 151.511 kb on + strand, within GFF1959 at 151.541 kb on - strand, within GFF1959 at 151.683 kb on + strand, within GFF1959 at 151.683 kb on + strand, within GFF1959 at 151.684 kb on - strand, within GFF1959 at 151.685 kb on + strand, within GFF1959 at 151.685 kb on + strand, within GFF1959 at 151.686 kb on - strand, within GFF1959 at 151.686 kb on - strand, within GFF1959 at 151.693 kb on + strand, within GFF1959 at 151.693 kb on + strand, within GFF1959 at 151.895 kb on + strand, within GFF1959 at 151.896 kb on - strand, within GFF1959
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 149,160 - GFF1957 0.29 -0.1 149,160 - GFF1957 0.29 -1.5 149,163 + GFF1957 0.29 +1.0 149,164 - GFF1957 0.29 -0.3 149,248 + GFF1957 0.35 +0.1 149,266 + GFF1957 0.37 -0.4 149,267 - GFF1957 0.37 -0.3 149,541 + GFF1957 0.58 +1.2 149,541 + GFF1957 0.58 +0.0 149,564 + GFF1957 0.60 +1.1 149,666 + GFF1957 0.67 -1.0 149,700 - GFF1957 0.70 -0.4 149,823 + GFF1957 0.79 -2.9 149,823 + GFF1957 0.79 +2.4 149,823 + GFF1957 0.79 -0.8 149,957 + GFF1957 0.90 +0.2 149,991 + +3.6 149,991 + -1.0 149,992 - -0.9 149,993 + +0.1 149,993 + +0.3 149,993 + -0.5 149,994 - +0.4 149,994 - +1.5 150,259 + GFF1958 0.14 -0.0 150,278 + GFF1958 0.17 -1.2 150,279 - GFF1958 0.17 -0.4 150,279 - GFF1958 0.17 +0.2 150,279 - GFF1958 0.17 -0.4 150,281 - GFF1958 0.17 -0.6 150,467 + GFF1958 0.42 +2.7 150,468 - GFF1958 0.42 -0.6 150,469 + GFF1958 0.42 +1.1 150,469 + GFF1958 0.42 -2.2 150,470 - GFF1958 0.42 -0.7 150,540 - GFF1958 0.51 +0.3 150,586 - GFF1958 0.57 -0.1 150,586 - GFF1958 0.57 +0.2 150,591 - GFF1958 0.58 +0.2 150,725 - GFF1958 0.76 +0.5 150,725 - GFF1958 0.76 -0.7 150,761 - GFF1958 0.80 -2.4 150,761 - GFF1958 0.80 +0.2 150,790 + GFF1958 0.84 -0.2 150,791 - GFF1958 0.84 -0.3 150,908 + -0.0 150,928 - +0.5 150,928 - +1.1 150,928 - +1.1 150,928 - -0.8 150,928 - +1.0 151,018 + +0.1 151,018 + -0.0 151,019 - +1.1 151,019 - +0.6 151,019 - +0.7 151,019 - +0.6 151,077 - -0.9 151,172 + -0.4 151,499 + GFF1959 0.32 -1.4 151,499 + GFF1959 0.32 +0.8 151,500 - GFF1959 0.32 -0.2 151,511 + GFF1959 0.33 -0.9 151,541 - GFF1959 0.37 -1.1 151,683 + GFF1959 0.52 -0.1 151,683 + GFF1959 0.52 -0.2 151,684 - GFF1959 0.52 +0.2 151,685 + GFF1959 0.52 +0.5 151,685 + GFF1959 0.52 -0.2 151,686 - GFF1959 0.52 -0.1 151,686 - GFF1959 0.52 -0.3 151,693 + GFF1959 0.53 -0.4 151,693 + GFF1959 0.53 +0.6 151,895 + GFF1959 0.74 -0.5 151,896 - GFF1959 0.74 -0.7
Or see this region's nucleotide sequence