Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF191 and GFF192 are separated by 10 nucleotides GFF192 and GFF193 are separated by 105 nucleotides GFF193 and GFF194 are separated by 198 nucleotides
GFF191 - ApaG protein, at 186,430 to 186,807
GFF191
GFF192 - 'Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41)' transl_table=11, at 186,818 to 187,666
GFF192
GFF193 - Dihydrofolate reductase (EC 1.5.1.3), at 187,772 to 188,251
GFF193
GFF194 - Glutathione-regulated potassium-efflux system protein KefC, at 188,450 to 190,312
GFF194
Position (kb) 187
188
189 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 186.811 kb on + strand at 186.855 kb on - strand at 186.892 kb on + strand at 186.892 kb on + strand at 186.963 kb on + strand, within GFF192 at 186.964 kb on - strand, within GFF192 at 186.969 kb on - strand, within GFF192 at 186.989 kb on - strand, within GFF192 at 187.064 kb on - strand, within GFF192 at 187.183 kb on + strand, within GFF192 at 187.336 kb on - strand, within GFF192 at 187.346 kb on - strand, within GFF192 at 187.346 kb on - strand, within GFF192 at 187.348 kb on + strand, within GFF192 at 187.400 kb on + strand, within GFF192 at 187.478 kb on + strand, within GFF192 at 187.479 kb on - strand, within GFF192 at 187.479 kb on - strand, within GFF192 at 187.605 kb on - strand at 187.605 kb on - strand at 188.288 kb on - strand at 188.391 kb on - strand at 188.391 kb on - strand at 188.414 kb on + strand at 188.415 kb on - strand at 188.805 kb on + strand, within GFF194 at 188.996 kb on - strand, within GFF194 at 189.011 kb on - strand, within GFF194 at 189.011 kb on - strand, within GFF194
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 186,811 + -1.7 186,855 - -2.9 186,892 + -0.5 186,892 + -1.5 186,963 + GFF192 0.17 -0.9 186,964 - GFF192 0.17 -3.8 186,969 - GFF192 0.18 -1.8 186,989 - GFF192 0.20 -0.9 187,064 - GFF192 0.29 -3.4 187,183 + GFF192 0.43 -3.1 187,336 - GFF192 0.61 -0.3 187,346 - GFF192 0.62 -4.0 187,346 - GFF192 0.62 -4.5 187,348 + GFF192 0.62 -0.6 187,400 + GFF192 0.69 -1.1 187,478 + GFF192 0.78 -2.8 187,479 - GFF192 0.78 -3.6 187,479 - GFF192 0.78 -1.0 187,605 - -2.3 187,605 - -2.3 188,288 - +0.2 188,391 - +1.1 188,391 - -0.4 188,414 + +0.4 188,415 - +0.3 188,805 + GFF194 0.19 +2.2 188,996 - GFF194 0.29 -0.2 189,011 - GFF194 0.30 +1.2 189,011 - GFF194 0.30 +2.4
Or see this region's nucleotide sequence