Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1846 and GFF1847 overlap by 4 nucleotides GFF1847 and GFF1848 are separated by 425 nucleotides
GFF1846 - Anaerobic glycerol-3-phosphate dehydrogenase subunit B (EC 1.1.5.3), at 38,247 to 39,506
GFF1846
GFF1847 - Anaerobic glycerol-3-phosphate dehydrogenase subunit C (EC 1.1.5.3), at 39,503 to 40,693
GFF1847
GFF1848 - FIG074102: hypothetical protein, at 41,119 to 42,141
GFF1848
Position (kb)
39
40
41 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 38.531 kb on + strand, within GFF1846 at 38.619 kb on - strand, within GFF1846 at 38.779 kb on + strand, within GFF1846 at 38.860 kb on - strand, within GFF1846 at 38.878 kb on + strand, within GFF1846 at 39.170 kb on - strand, within GFF1846 at 39.279 kb on + strand, within GFF1846 at 39.382 kb on - strand at 39.388 kb on + strand at 39.389 kb on - strand at 39.389 kb on - strand at 39.418 kb on + strand at 39.418 kb on + strand at 39.418 kb on + strand at 39.419 kb on - strand at 39.531 kb on + strand at 39.674 kb on - strand, within GFF1847 at 39.724 kb on + strand, within GFF1847 at 40.135 kb on + strand, within GFF1847 at 40.204 kb on + strand, within GFF1847 at 40.234 kb on + strand, within GFF1847 at 40.235 kb on - strand, within GFF1847 at 40.301 kb on - strand, within GFF1847 at 40.385 kb on + strand, within GFF1847 at 40.385 kb on + strand, within GFF1847 at 40.385 kb on + strand, within GFF1847 at 40.391 kb on - strand, within GFF1847 at 40.473 kb on + strand, within GFF1847 at 40.639 kb on + strand at 40.695 kb on + strand at 40.763 kb on - strand at 40.772 kb on - strand at 40.772 kb on - strand at 40.812 kb on + strand at 40.848 kb on - strand at 40.906 kb on + strand at 40.916 kb on + strand at 41.174 kb on + strand at 41.562 kb on - strand, within GFF1848
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 38,531 + GFF1846 0.23 -0.1 38,619 - GFF1846 0.30 -0.2 38,779 + GFF1846 0.42 +0.5 38,860 - GFF1846 0.49 -0.8 38,878 + GFF1846 0.50 +0.8 39,170 - GFF1846 0.73 +0.3 39,279 + GFF1846 0.82 -0.3 39,382 - +0.5 39,388 + +0.3 39,389 - -0.3 39,389 - +0.9 39,418 + +1.6 39,418 + -0.3 39,418 + +2.3 39,419 - -0.8 39,531 + +2.2 39,674 - GFF1847 0.14 +0.6 39,724 + GFF1847 0.19 -1.1 40,135 + GFF1847 0.53 +0.2 40,204 + GFF1847 0.59 -0.4 40,234 + GFF1847 0.61 +1.5 40,235 - GFF1847 0.61 -0.1 40,301 - GFF1847 0.67 +0.5 40,385 + GFF1847 0.74 +1.0 40,385 + GFF1847 0.74 -0.1 40,385 + GFF1847 0.74 +0.3 40,391 - GFF1847 0.75 +0.5 40,473 + GFF1847 0.81 +0.5 40,639 + +1.8 40,695 + +2.4 40,763 - +0.2 40,772 - -1.8 40,772 - -0.3 40,812 + -0.3 40,848 - -0.0 40,906 + -0.5 40,916 + +1.7 41,174 + +0.9 41,562 - GFF1848 0.43 +0.0
Or see this region's nucleotide sequence