Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1708 and GFF1709 are separated by 7 nucleotides GFF1709 and GFF1710 are separated by 196 nucleotides GFF1710 and GFF1711 are separated by 347 nucleotides
GFF1708 - Ribose ABC transport system, ATP-binding protein RbsA (TC 3.A.1.2.1), at 6,248 to 7,753
GFF1708
GFF1709 - Ribose ABC transport system, high affinity permease RbsD (TC 3.A.1.2.1), at 7,761 to 8,180
GFF1709
GFF1710 - Kup system potassium uptake protein, at 8,377 to 10,245
GFF1710
GFF1711 - Putative regulator protein, at 10,593 to 12,089
GFF1711
Position (kb)
8
9
10
11 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 7.505 kb on - strand, within GFF1708 at 7.593 kb on + strand, within GFF1708 at 7.593 kb on + strand, within GFF1708 at 7.594 kb on - strand, within GFF1708 at 7.605 kb on - strand at 7.720 kb on + strand at 8.027 kb on + strand, within GFF1709 at 8.028 kb on - strand, within GFF1709 at 8.115 kb on - strand, within GFF1709 at 8.121 kb on - strand, within GFF1709 at 8.163 kb on + strand at 8.163 kb on + strand at 8.222 kb on - strand at 8.240 kb on - strand at 8.307 kb on + strand at 8.322 kb on + strand at 8.394 kb on + strand at 8.399 kb on - strand at 8.496 kb on - strand at 8.535 kb on + strand at 8.565 kb on + strand, within GFF1710 at 8.565 kb on + strand, within GFF1710 at 8.566 kb on - strand, within GFF1710 at 8.566 kb on - strand, within GFF1710 at 8.689 kb on - strand, within GFF1710 at 8.696 kb on + strand, within GFF1710 at 8.696 kb on + strand, within GFF1710 at 8.773 kb on + strand, within GFF1710 at 8.810 kb on + strand, within GFF1710 at 8.810 kb on + strand, within GFF1710 at 8.810 kb on + strand, within GFF1710 at 8.810 kb on + strand, within GFF1710 at 8.811 kb on - strand, within GFF1710 at 8.811 kb on - strand, within GFF1710 at 8.811 kb on - strand, within GFF1710 at 8.811 kb on - strand, within GFF1710 at 8.811 kb on - strand, within GFF1710 at 8.811 kb on - strand, within GFF1710 at 8.813 kb on - strand, within GFF1710 at 8.895 kb on + strand, within GFF1710 at 8.994 kb on + strand, within GFF1710 at 9.063 kb on - strand, within GFF1710 at 9.085 kb on - strand, within GFF1710 at 9.108 kb on + strand, within GFF1710 at 9.147 kb on - strand, within GFF1710 at 9.206 kb on + strand, within GFF1710 at 9.207 kb on - strand, within GFF1710 at 9.233 kb on + strand, within GFF1710 at 9.234 kb on - strand, within GFF1710 at 9.262 kb on - strand, within GFF1710 at 9.268 kb on - strand, within GFF1710 at 9.287 kb on + strand, within GFF1710 at 9.444 kb on + strand, within GFF1710 at 9.445 kb on - strand, within GFF1710 at 9.445 kb on - strand, within GFF1710 at 9.448 kb on + strand, within GFF1710 at 9.551 kb on + strand, within GFF1710 at 9.552 kb on - strand, within GFF1710 at 9.601 kb on - strand, within GFF1710 at 9.616 kb on - strand, within GFF1710 at 9.661 kb on + strand, within GFF1710 at 9.662 kb on - strand, within GFF1710 at 9.684 kb on + strand, within GFF1710 at 9.751 kb on + strand, within GFF1710 at 9.752 kb on - strand, within GFF1710 at 9.937 kb on - strand, within GFF1710 at 9.940 kb on + strand, within GFF1710 at 9.941 kb on - strand, within GFF1710 at 9.966 kb on + strand, within GFF1710 at 9.967 kb on - strand, within GFF1710 at 10.043 kb on + strand, within GFF1710 at 10.156 kb on - strand at 10.157 kb on + strand at 10.158 kb on - strand at 10.198 kb on - strand at 10.198 kb on - strand at 10.262 kb on + strand at 10.264 kb on + strand at 10.386 kb on - strand at 10.387 kb on + strand at 10.388 kb on - strand at 10.388 kb on - strand at 10.388 kb on - strand at 10.388 kb on - strand at 10.388 kb on - strand at 10.391 kb on + strand at 10.392 kb on - strand at 10.406 kb on + strand at 10.446 kb on + strand at 10.481 kb on + strand at 10.521 kb on + strand at 10.544 kb on + strand at 10.545 kb on - strand at 10.556 kb on + strand at 10.557 kb on - strand at 10.667 kb on + strand at 10.668 kb on - strand at 10.668 kb on - strand at 10.690 kb on + strand at 10.958 kb on + strand, within GFF1711 at 11.226 kb on + strand, within GFF1711
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 7,505 - GFF1708 0.83 -0.4 7,593 + GFF1708 0.89 -0.4 7,593 + GFF1708 0.89 -0.5 7,594 - GFF1708 0.89 -0.0 7,605 - +2.1 7,720 + -0.4 8,027 + GFF1709 0.63 +1.8 8,028 - GFF1709 0.64 +0.7 8,115 - GFF1709 0.84 -0.3 8,121 - GFF1709 0.86 +0.1 8,163 + +0.3 8,163 + -1.2 8,222 - -1.8 8,240 - -0.0 8,307 + -0.3 8,322 + +1.8 8,394 + +0.7 8,399 - +2.6 8,496 - +0.1 8,535 + -0.4 8,565 + GFF1710 0.10 -0.2 8,565 + GFF1710 0.10 +0.6 8,566 - GFF1710 0.10 +0.4 8,566 - GFF1710 0.10 +0.8 8,689 - GFF1710 0.17 +1.2 8,696 + GFF1710 0.17 -0.7 8,696 + GFF1710 0.17 +1.6 8,773 + GFF1710 0.21 +0.0 8,810 + GFF1710 0.23 -0.2 8,810 + GFF1710 0.23 -0.7 8,810 + GFF1710 0.23 +0.3 8,810 + GFF1710 0.23 +1.1 8,811 - GFF1710 0.23 +0.8 8,811 - GFF1710 0.23 -0.8 8,811 - GFF1710 0.23 -0.6 8,811 - GFF1710 0.23 -0.3 8,811 - GFF1710 0.23 -0.3 8,811 - GFF1710 0.23 +0.5 8,813 - GFF1710 0.23 +0.2 8,895 + GFF1710 0.28 +0.6 8,994 + GFF1710 0.33 -0.7 9,063 - GFF1710 0.37 +0.5 9,085 - GFF1710 0.38 -1.4 9,108 + GFF1710 0.39 -1.2 9,147 - GFF1710 0.41 +0.2 9,206 + GFF1710 0.44 -0.4 9,207 - GFF1710 0.44 -0.5 9,233 + GFF1710 0.46 +0.5 9,234 - GFF1710 0.46 +0.2 9,262 - GFF1710 0.47 +0.3 9,268 - GFF1710 0.48 -0.5 9,287 + GFF1710 0.49 +1.3 9,444 + GFF1710 0.57 +0.1 9,445 - GFF1710 0.57 -0.6 9,445 - GFF1710 0.57 +0.5 9,448 + GFF1710 0.57 -1.5 9,551 + GFF1710 0.63 +0.8 9,552 - GFF1710 0.63 -0.6 9,601 - GFF1710 0.65 -0.1 9,616 - GFF1710 0.66 +2.6 9,661 + GFF1710 0.69 -0.2 9,662 - GFF1710 0.69 -0.5 9,684 + GFF1710 0.70 -1.0 9,751 + GFF1710 0.74 +0.9 9,752 - GFF1710 0.74 -1.0 9,937 - GFF1710 0.83 -0.0 9,940 + GFF1710 0.84 -0.4 9,941 - GFF1710 0.84 +0.4 9,966 + GFF1710 0.85 -0.1 9,967 - GFF1710 0.85 -0.5 10,043 + GFF1710 0.89 -1.7 10,156 - -0.0 10,157 + -1.0 10,158 - +0.4 10,198 - +0.5 10,198 - -0.1 10,262 + -1.3 10,264 + +4.1 10,386 - +0.3 10,387 + +0.5 10,388 - +0.3 10,388 - +0.2 10,388 - +0.5 10,388 - +0.6 10,388 - +0.0 10,391 + +0.0 10,392 - -0.4 10,406 + -0.6 10,446 + -0.4 10,481 + +0.2 10,521 + +2.5 10,544 + -0.5 10,545 - +1.1 10,556 + +0.2 10,557 - -0.5 10,667 + -0.2 10,668 - +0.5 10,668 - +0.6 10,690 + -0.4 10,958 + GFF1711 0.24 +0.3 11,226 + GFF1711 0.42 -0.8
Or see this region's nucleotide sequence