Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1684 and GFF1685 are separated by 237 nucleotides GFF1685 and GFF1686 are separated by 212 nucleotides GFF1686 and GFF1687 are separated by 218 nucleotides GFF1687 and GFF1688 are separated by 264 nucleotides GFF1688 and GFF1689 are separated by 11 nucleotides
GFF1684 - FIG00637942: hypothetical protein, at 68,107 to 68,772
GFF1684
GFF1685 - FIG01045540: hypothetical protein, at 69,010 to 69,336
GFF1685
GFF1686 - FIG01200701: possible membrane protein, at 69,549 to 70,982
GFF1686
GFF1687 - Putative inner membrane protein, at 71,201 to 71,518
GFF1687
GFF1688 - tRNA-Leu-TAA, at 71,783 to 71,869
GFF1688
GFF1689 - tRNA-Cys-GCA, at 71,881 to 71,954
GFF1689
Position (kb)
69
70
71 Strain fitness (log2 ratio)
-2
-1
0
1 at 68.588 kb on + strand, within GFF1684 at 68.712 kb on - strand at 68.804 kb on + strand at 68.993 kb on - strand at 69.033 kb on - strand at 69.091 kb on + strand, within GFF1685 at 69.219 kb on - strand, within GFF1685 at 69.322 kb on - strand at 69.393 kb on + strand at 69.491 kb on + strand at 69.493 kb on + strand at 69.559 kb on + strand at 69.666 kb on - strand at 69.668 kb on + strand at 69.668 kb on + strand at 69.749 kb on - strand, within GFF1686 at 70.154 kb on + strand, within GFF1686 at 70.235 kb on + strand, within GFF1686 at 70.236 kb on - strand, within GFF1686 at 70.331 kb on - strand, within GFF1686 at 70.365 kb on - strand, within GFF1686 at 70.402 kb on - strand, within GFF1686 at 70.519 kb on + strand, within GFF1686 at 70.523 kb on + strand, within GFF1686 at 70.578 kb on - strand, within GFF1686 at 70.580 kb on - strand, within GFF1686 at 70.793 kb on - strand, within GFF1686 at 70.820 kb on + strand, within GFF1686 at 71.011 kb on - strand at 71.061 kb on + strand at 71.191 kb on - strand at 71.767 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 68,588 + GFF1684 0.72 +0.9 68,712 - +1.7 68,804 + +0.0 68,993 - -0.3 69,033 - +0.2 69,091 + GFF1685 0.25 -0.3 69,219 - GFF1685 0.64 +0.6 69,322 - -0.5 69,393 + -1.9 69,491 + -1.6 69,493 + -0.8 69,559 + +0.0 69,666 - +1.3 69,668 + -0.5 69,668 + +0.5 69,749 - GFF1686 0.14 +1.3 70,154 + GFF1686 0.42 +0.2 70,235 + GFF1686 0.48 +0.2 70,236 - GFF1686 0.48 -0.7 70,331 - GFF1686 0.55 +0.9 70,365 - GFF1686 0.57 -1.9 70,402 - GFF1686 0.59 +0.7 70,519 + GFF1686 0.68 -1.1 70,523 + GFF1686 0.68 +0.0 70,578 - GFF1686 0.72 -0.3 70,580 - GFF1686 0.72 -1.7 70,793 - GFF1686 0.87 +1.0 70,820 + GFF1686 0.89 -1.6 71,011 - -1.8 71,061 + -1.5 71,191 - -2.7 71,767 - +0.5
Or see this region's nucleotide sequence