Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1653 and GFF1654 are separated by 19 nucleotides GFF1654 and GFF1655 are separated by 104 nucleotides GFF1655 and GFF1656 are separated by 15 nucleotides GFF1656 and GFF1657 are separated by 188 nucleotides
GFF1653 - Cytoplasmic copper homeostasis protein CutC, at 37,446 to 38,192
GFF1653
GFF1654 - Protein yecM, at 38,212 to 38,781
GFF1654
GFF1655 - hypothetical protein, at 38,886 to 39,002
GFF1655
GFF1656 - Arginyl-tRNA synthetase (EC 6.1.1.19), at 39,018 to 40,751
GFF1656
GFF1657 - Penicillin-binding protein 2 (PBP-2), at 40,940 to 42,811
GFF1657
Position (kb) 39
40
41 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 38.031 kb on + strand, within GFF1653 at 38.042 kb on + strand, within GFF1653 at 38.046 kb on - strand, within GFF1653 at 38.065 kb on - strand, within GFF1653 at 38.185 kb on - strand at 38.301 kb on + strand, within GFF1654 at 38.347 kb on - strand, within GFF1654 at 38.367 kb on + strand, within GFF1654 at 38.571 kb on + strand, within GFF1654 at 38.584 kb on + strand, within GFF1654 at 38.585 kb on - strand, within GFF1654 at 38.865 kb on - strand at 38.876 kb on - strand at 40.749 kb on + strand at 40.825 kb on - strand at 40.858 kb on + strand at 40.860 kb on + strand at 41.047 kb on + strand at 41.076 kb on - strand at 41.171 kb on - strand, within GFF1657 at 41.176 kb on - strand, within GFF1657 at 41.184 kb on - strand, within GFF1657 at 41.184 kb on - strand, within GFF1657 at 41.207 kb on - strand, within GFF1657 at 41.408 kb on + strand, within GFF1657 at 41.408 kb on + strand, within GFF1657 at 41.408 kb on + strand, within GFF1657 at 41.409 kb on - strand, within GFF1657 at 41.489 kb on - strand, within GFF1657 at 41.545 kb on + strand, within GFF1657 at 41.629 kb on + strand, within GFF1657
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 38,031 + GFF1653 0.78 +0.2 38,042 + GFF1653 0.80 +0.2 38,046 - GFF1653 0.80 -0.1 38,065 - GFF1653 0.83 +1.0 38,185 - +3.1 38,301 + GFF1654 0.16 -0.8 38,347 - GFF1654 0.24 +0.4 38,367 + GFF1654 0.27 +0.9 38,571 + GFF1654 0.63 +0.1 38,584 + GFF1654 0.65 +1.4 38,585 - GFF1654 0.65 -0.3 38,865 - +0.7 38,876 - +0.9 40,749 + -0.8 40,825 - -0.1 40,858 + -0.7 40,860 + -0.3 41,047 + -0.2 41,076 - -0.6 41,171 - GFF1657 0.12 +0.2 41,176 - GFF1657 0.13 -1.4 41,184 - GFF1657 0.13 -1.1 41,184 - GFF1657 0.13 +0.0 41,207 - GFF1657 0.14 +0.3 41,408 + GFF1657 0.25 -0.2 41,408 + GFF1657 0.25 -2.2 41,408 + GFF1657 0.25 -1.3 41,409 - GFF1657 0.25 -0.2 41,489 - GFF1657 0.29 +4.3 41,545 + GFF1657 0.32 -1.1 41,629 + GFF1657 0.37 -0.7
Or see this region's nucleotide sequence