Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1546 and GFF1547 are separated by 109 nucleotides GFF1547 and GFF1548 are separated by 31 nucleotides
GFF1546 - ABC transporter protein IroC, at 5,755 to 9,408
GFF1546
GFF1547 - Trilactone hydrolase IroD, at 9,518 to 10,762
GFF1547
GFF1548 - Periplasmic esterase IroE, at 10,794 to 11,729
GFF1548
Position (kb)
9
10
11 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 8.578 kb on - strand, within GFF1546 at 8.909 kb on + strand, within GFF1546 at 9.343 kb on + strand at 9.402 kb on - strand at 9.402 kb on - strand at 9.476 kb on + strand at 9.510 kb on + strand at 9.775 kb on + strand, within GFF1547 at 9.776 kb on - strand, within GFF1547 at 9.803 kb on - strand, within GFF1547 at 9.850 kb on + strand, within GFF1547 at 9.961 kb on - strand, within GFF1547 at 10.106 kb on + strand, within GFF1547 at 10.255 kb on + strand, within GFF1547 at 10.255 kb on + strand, within GFF1547 at 10.588 kb on + strand, within GFF1547 at 10.682 kb on + strand at 10.710 kb on - strand at 10.743 kb on + strand at 10.798 kb on - strand at 10.798 kb on - strand at 10.930 kb on - strand, within GFF1548 at 11.038 kb on + strand, within GFF1548 at 11.040 kb on + strand, within GFF1548 at 11.040 kb on + strand, within GFF1548 at 11.040 kb on + strand, within GFF1548 at 11.040 kb on + strand, within GFF1548 at 11.040 kb on + strand, within GFF1548 at 11.040 kb on + strand, within GFF1548 at 11.041 kb on - strand, within GFF1548 at 11.041 kb on - strand, within GFF1548 at 11.042 kb on + strand, within GFF1548 at 11.042 kb on + strand, within GFF1548 at 11.042 kb on + strand, within GFF1548 at 11.042 kb on + strand, within GFF1548 at 11.042 kb on + strand, within GFF1548 at 11.145 kb on - strand, within GFF1548 at 11.175 kb on + strand, within GFF1548 at 11.176 kb on - strand, within GFF1548 at 11.176 kb on - strand, within GFF1548 at 11.176 kb on - strand, within GFF1548 at 11.285 kb on + strand, within GFF1548 at 11.286 kb on - strand, within GFF1548 at 11.352 kb on + strand, within GFF1548 at 11.402 kb on + strand, within GFF1548 at 11.402 kb on + strand, within GFF1548 at 11.403 kb on - strand, within GFF1548 at 11.406 kb on + strand, within GFF1548 at 11.407 kb on - strand, within GFF1548 at 11.410 kb on - strand, within GFF1548 at 11.410 kb on - strand, within GFF1548 at 11.439 kb on - strand, within GFF1548 at 11.462 kb on + strand, within GFF1548 at 11.507 kb on - strand, within GFF1548
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 8,578 - GFF1546 0.77 +0.5 8,909 + GFF1546 0.86 +0.6 9,343 + +0.0 9,402 - +1.1 9,402 - -0.9 9,476 + +1.2 9,510 + -0.2 9,775 + GFF1547 0.21 -0.1 9,776 - GFF1547 0.21 +4.3 9,803 - GFF1547 0.23 +0.4 9,850 + GFF1547 0.27 -1.0 9,961 - GFF1547 0.36 +1.0 10,106 + GFF1547 0.47 -0.7 10,255 + GFF1547 0.59 -0.0 10,255 + GFF1547 0.59 -0.9 10,588 + GFF1547 0.86 -1.0 10,682 + +0.4 10,710 - -0.3 10,743 + -0.1 10,798 - +0.9 10,798 - +2.6 10,930 - GFF1548 0.15 +0.2 11,038 + GFF1548 0.26 -1.2 11,040 + GFF1548 0.26 +0.8 11,040 + GFF1548 0.26 -0.0 11,040 + GFF1548 0.26 +0.3 11,040 + GFF1548 0.26 -0.2 11,040 + GFF1548 0.26 +0.6 11,040 + GFF1548 0.26 +0.7 11,041 - GFF1548 0.26 +0.4 11,041 - GFF1548 0.26 +0.8 11,042 + GFF1548 0.26 -1.2 11,042 + GFF1548 0.26 +2.5 11,042 + GFF1548 0.26 +1.6 11,042 + GFF1548 0.26 -0.7 11,042 + GFF1548 0.26 -1.7 11,145 - GFF1548 0.38 +0.4 11,175 + GFF1548 0.41 -1.1 11,176 - GFF1548 0.41 -0.8 11,176 - GFF1548 0.41 -0.2 11,176 - GFF1548 0.41 +0.6 11,285 + GFF1548 0.52 -0.4 11,286 - GFF1548 0.53 -1.5 11,352 + GFF1548 0.60 -0.3 11,402 + GFF1548 0.65 -0.6 11,402 + GFF1548 0.65 +0.2 11,403 - GFF1548 0.65 -0.2 11,406 + GFF1548 0.65 +0.9 11,407 - GFF1548 0.65 -0.4 11,410 - GFF1548 0.66 +0.5 11,410 - GFF1548 0.66 +0.3 11,439 - GFF1548 0.69 +1.4 11,462 + GFF1548 0.71 +0.3 11,507 - GFF1548 0.76 +0.6
Or see this region's nucleotide sequence