Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1377 and GFF1378 overlap by 4 nucleotides GFF1378 and GFF1379 are separated by 298 nucleotides GFF1379 and GFF1380 are separated by 8 nucleotides GFF1380 and GFF1381 are separated by 9 nucleotides
GFF1377 - Polymyxin resistance protein ArnC, glycosyl transferase (EC 2.4.-.-), at 26,121 to 27,050
GFF1377
GFF1378 - putative phage glucose translocase, at 27,047 to 27,304
GFF1378
GFF1379 - hypothetical protein, at 27,603 to 27,728
GFF1379
GFF1380 - Putative phage baseplate component, at 27,737 to 28,459
GFF1380
GFF1381 - Gene D protein, at 28,469 to 29,512
GFF1381
Position (kb) 27
28
29 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 26.943 kb on - strand, within GFF1377 at 27.143 kb on - strand, within GFF1378 at 27.201 kb on + strand, within GFF1378 at 27.210 kb on + strand, within GFF1378 at 27.224 kb on + strand, within GFF1378 at 27.226 kb on - strand, within GFF1378 at 27.388 kb on - strand at 27.399 kb on - strand at 27.536 kb on + strand at 27.540 kb on + strand at 27.540 kb on + strand at 27.812 kb on - strand, within GFF1380 at 27.984 kb on - strand, within GFF1380 at 27.984 kb on - strand, within GFF1380 at 28.062 kb on - strand, within GFF1380 at 28.075 kb on + strand, within GFF1380 at 28.076 kb on - strand, within GFF1380 at 28.076 kb on - strand, within GFF1380 at 28.076 kb on - strand, within GFF1380 at 28.084 kb on + strand, within GFF1380 at 28.233 kb on + strand, within GFF1380 at 28.360 kb on + strand, within GFF1380 at 28.459 kb on + strand at 28.459 kb on + strand at 28.460 kb on - strand at 28.595 kb on + strand, within GFF1381 at 28.596 kb on - strand, within GFF1381 at 28.864 kb on + strand, within GFF1381 at 28.865 kb on - strand, within GFF1381 at 28.879 kb on - strand, within GFF1381 at 28.980 kb on + strand, within GFF1381 at 28.981 kb on - strand, within GFF1381 at 28.981 kb on - strand, within GFF1381 at 29.126 kb on - strand, within GFF1381 at 29.241 kb on + strand, within GFF1381 at 29.381 kb on - strand, within GFF1381 at 29.384 kb on - strand, within GFF1381 at 29.413 kb on - strand at 29.413 kb on - strand at 29.427 kb on + strand at 29.441 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 26,943 - GFF1377 0.88 +3.5 27,143 - GFF1378 0.37 +0.8 27,201 + GFF1378 0.60 -0.4 27,210 + GFF1378 0.63 -0.1 27,224 + GFF1378 0.69 -0.5 27,226 - GFF1378 0.69 +1.0 27,388 - -0.5 27,399 - +5.4 27,536 + -1.8 27,540 + +0.8 27,540 + +2.1 27,812 - GFF1380 0.10 -1.8 27,984 - GFF1380 0.34 +0.8 27,984 - GFF1380 0.34 +0.5 28,062 - GFF1380 0.45 +0.1 28,075 + GFF1380 0.47 -0.1 28,076 - GFF1380 0.47 +0.4 28,076 - GFF1380 0.47 -0.7 28,076 - GFF1380 0.47 -0.1 28,084 + GFF1380 0.48 -0.9 28,233 + GFF1380 0.69 +0.1 28,360 + GFF1380 0.86 +0.1 28,459 + +3.4 28,459 + -1.6 28,460 - +0.1 28,595 + GFF1381 0.12 +0.2 28,596 - GFF1381 0.12 +1.8 28,864 + GFF1381 0.38 +1.9 28,865 - GFF1381 0.38 +0.6 28,879 - GFF1381 0.39 +0.2 28,980 + GFF1381 0.49 +1.9 28,981 - GFF1381 0.49 -0.2 28,981 - GFF1381 0.49 -0.1 29,126 - GFF1381 0.63 -1.6 29,241 + GFF1381 0.74 -0.6 29,381 - GFF1381 0.87 +0.5 29,384 - GFF1381 0.88 +0.1 29,413 - -0.8 29,413 - -0.4 29,427 + +0.2 29,441 - +1.0
Or see this region's nucleotide sequence