Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1338 and GFF1339 overlap by 4 nucleotides GFF1339 and GFF1340 are separated by 40 nucleotides GFF1340 and GFF1341 are separated by 119 nucleotides
GFF1338 - Putative outer membrane protein, at 45,462 to 46,538
GFF1338
GFF1339 - Probable fimbrial chaperone protein, at 46,535 to 47,278
GFF1339
GFF1340 - FIG036507: Fimbriae usher protein StdB, at 47,319 to 49,808
GFF1340
GFF1341 - Putative fimbrial-like protein, at 49,928 to 50,512
GFF1341
Position (kb)
47
48
49
50 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 46.439 kb on + strand at 46.439 kb on + strand at 46.443 kb on + strand at 46.443 kb on + strand at 46.621 kb on + strand, within GFF1339 at 46.621 kb on + strand, within GFF1339 at 46.622 kb on - strand, within GFF1339 at 46.622 kb on - strand, within GFF1339 at 46.623 kb on + strand, within GFF1339 at 46.668 kb on - strand, within GFF1339 at 46.668 kb on - strand, within GFF1339 at 46.730 kb on - strand, within GFF1339 at 46.761 kb on + strand, within GFF1339 at 46.764 kb on + strand, within GFF1339 at 46.850 kb on - strand, within GFF1339 at 46.867 kb on - strand, within GFF1339 at 46.867 kb on - strand, within GFF1339 at 46.981 kb on + strand, within GFF1339 at 46.981 kb on + strand, within GFF1339 at 46.981 kb on + strand, within GFF1339 at 46.982 kb on - strand, within GFF1339 at 46.982 kb on - strand, within GFF1339 at 47.038 kb on + strand, within GFF1339 at 47.179 kb on + strand, within GFF1339 at 47.180 kb on - strand, within GFF1339 at 47.314 kb on + strand at 47.321 kb on - strand at 47.681 kb on + strand, within GFF1340 at 47.681 kb on + strand, within GFF1340 at 47.787 kb on + strand, within GFF1340 at 47.787 kb on + strand, within GFF1340 at 47.788 kb on - strand, within GFF1340 at 47.788 kb on - strand, within GFF1340 at 47.788 kb on - strand, within GFF1340 at 48.088 kb on + strand, within GFF1340 at 48.088 kb on + strand, within GFF1340 at 48.089 kb on - strand, within GFF1340 at 48.089 kb on - strand, within GFF1340 at 48.120 kb on - strand, within GFF1340 at 48.163 kb on + strand, within GFF1340 at 48.244 kb on + strand, within GFF1340 at 48.245 kb on - strand, within GFF1340 at 48.245 kb on - strand, within GFF1340 at 48.245 kb on - strand, within GFF1340 at 48.248 kb on + strand, within GFF1340 at 48.332 kb on - strand, within GFF1340 at 48.332 kb on - strand, within GFF1340 at 48.398 kb on - strand, within GFF1340 at 48.468 kb on - strand, within GFF1340 at 48.485 kb on - strand, within GFF1340 at 48.485 kb on - strand, within GFF1340 at 48.496 kb on + strand, within GFF1340 at 48.582 kb on - strand, within GFF1340 at 48.659 kb on + strand, within GFF1340 at 48.659 kb on + strand, within GFF1340 at 48.782 kb on + strand, within GFF1340 at 48.866 kb on + strand, within GFF1340 at 48.959 kb on + strand, within GFF1340 at 48.960 kb on - strand, within GFF1340 at 48.992 kb on - strand, within GFF1340 at 49.067 kb on + strand, within GFF1340 at 49.081 kb on + strand, within GFF1340 at 49.082 kb on - strand, within GFF1340 at 49.082 kb on - strand, within GFF1340 at 49.135 kb on + strand, within GFF1340 at 49.136 kb on - strand, within GFF1340 at 49.136 kb on - strand, within GFF1340 at 49.136 kb on - strand, within GFF1340 at 49.259 kb on - strand, within GFF1340 at 49.291 kb on + strand, within GFF1340 at 49.650 kb on - strand at 49.651 kb on + strand at 49.652 kb on - strand at 49.652 kb on - strand at 49.704 kb on - strand at 49.868 kb on + strand at 49.869 kb on - strand at 49.947 kb on + strand at 49.948 kb on - strand at 49.948 kb on - strand at 50.080 kb on + strand, within GFF1341 at 50.080 kb on + strand, within GFF1341 at 50.148 kb on + strand, within GFF1341 at 50.165 kb on - strand, within GFF1341 at 50.193 kb on - strand, within GFF1341 at 50.228 kb on - strand, within GFF1341 at 50.303 kb on - strand, within GFF1341 at 50.466 kb on + strand at 50.466 kb on + strand at 50.467 kb on - strand at 50.467 kb on - strand at 50.517 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 46,439 + +2.1 46,439 + -0.9 46,443 + +0.2 46,443 + -0.1 46,621 + GFF1339 0.12 +3.3 46,621 + GFF1339 0.12 -0.2 46,622 - GFF1339 0.12 +0.0 46,622 - GFF1339 0.12 +0.4 46,623 + GFF1339 0.12 -0.5 46,668 - GFF1339 0.18 -0.5 46,668 - GFF1339 0.18 +0.3 46,730 - GFF1339 0.26 +0.6 46,761 + GFF1339 0.30 +0.9 46,764 + GFF1339 0.31 -0.3 46,850 - GFF1339 0.42 -0.5 46,867 - GFF1339 0.45 +0.8 46,867 - GFF1339 0.45 -0.8 46,981 + GFF1339 0.60 +0.9 46,981 + GFF1339 0.60 -0.1 46,981 + GFF1339 0.60 -0.3 46,982 - GFF1339 0.60 -2.2 46,982 - GFF1339 0.60 +0.6 47,038 + GFF1339 0.68 -0.2 47,179 + GFF1339 0.87 -1.3 47,180 - GFF1339 0.87 -1.5 47,314 + -0.2 47,321 - +0.3 47,681 + GFF1340 0.15 -0.4 47,681 + GFF1340 0.15 +1.0 47,787 + GFF1340 0.19 +0.8 47,787 + GFF1340 0.19 +0.1 47,788 - GFF1340 0.19 +0.2 47,788 - GFF1340 0.19 -1.4 47,788 - GFF1340 0.19 -0.3 48,088 + GFF1340 0.31 +0.8 48,088 + GFF1340 0.31 +0.3 48,089 - GFF1340 0.31 -0.2 48,089 - GFF1340 0.31 -0.1 48,120 - GFF1340 0.32 -0.3 48,163 + GFF1340 0.34 +0.2 48,244 + GFF1340 0.37 -0.7 48,245 - GFF1340 0.37 -0.3 48,245 - GFF1340 0.37 +2.8 48,245 - GFF1340 0.37 +2.3 48,248 + GFF1340 0.37 +1.4 48,332 - GFF1340 0.41 -0.2 48,332 - GFF1340 0.41 +2.3 48,398 - GFF1340 0.43 +1.3 48,468 - GFF1340 0.46 -0.8 48,485 - GFF1340 0.47 -0.6 48,485 - GFF1340 0.47 -0.4 48,496 + GFF1340 0.47 -0.0 48,582 - GFF1340 0.51 +0.4 48,659 + GFF1340 0.54 -0.3 48,659 + GFF1340 0.54 +0.8 48,782 + GFF1340 0.59 +0.3 48,866 + GFF1340 0.62 +0.1 48,959 + GFF1340 0.66 -0.2 48,960 - GFF1340 0.66 -0.6 48,992 - GFF1340 0.67 -0.1 49,067 + GFF1340 0.70 +0.7 49,081 + GFF1340 0.71 -0.3 49,082 - GFF1340 0.71 -0.0 49,082 - GFF1340 0.71 -0.2 49,135 + GFF1340 0.73 +1.2 49,136 - GFF1340 0.73 +3.0 49,136 - GFF1340 0.73 -0.4 49,136 - GFF1340 0.73 +1.1 49,259 - GFF1340 0.78 +0.6 49,291 + GFF1340 0.79 -1.3 49,650 - +0.0 49,651 + -0.1 49,652 - +2.9 49,652 - -0.0 49,704 - +1.0 49,868 + -0.2 49,869 - +0.5 49,947 + -0.7 49,948 - -0.2 49,948 - +0.1 50,080 + GFF1341 0.26 +0.2 50,080 + GFF1341 0.26 +0.2 50,148 + GFF1341 0.38 -0.9 50,165 - GFF1341 0.41 +1.2 50,193 - GFF1341 0.45 -0.8 50,228 - GFF1341 0.51 -1.0 50,303 - GFF1341 0.64 +0.7 50,466 + +3.5 50,466 + -0.2 50,467 - +0.5 50,467 - +0.8 50,517 - +0.3
Or see this region's nucleotide sequence