Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1104 and GFF1105 are separated by 31 nucleotides GFF1105 and GFF1106 are separated by 64 nucleotides GFF1106 and GFF1107 are separated by 38 nucleotides GFF1107 and GFF1108 are separated by 99 nucleotides
GFF1104 - type 1 fimbrae adaptor subunit FimF, at 290,778 to 291,296
GFF1104
GFF1105 - Hypothetical fimbrial chaperone ycbF precursor, at 291,328 to 291,993
GFF1105
GFF1106 - FIG01200701: possible membrane protein, at 292,058 to 292,903
GFF1106
GFF1107 - Putative membrane protein, at 292,942 to 293,229
GFF1107
GFF1108 - Putative membrane protein, at 293,329 to 293,778
GFF1108
Position (kb) 292
293 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 291.060 kb on - strand, within GFF1104 at 291.071 kb on - strand, within GFF1104 at 291.071 kb on - strand, within GFF1104 at 291.134 kb on + strand, within GFF1104 at 291.294 kb on + strand at 291.317 kb on + strand at 291.320 kb on - strand at 291.320 kb on - strand at 291.381 kb on + strand at 291.386 kb on - strand at 291.505 kb on - strand, within GFF1105 at 291.577 kb on + strand, within GFF1105 at 291.577 kb on + strand, within GFF1105 at 291.743 kb on - strand, within GFF1105 at 291.805 kb on + strand, within GFF1105 at 291.805 kb on + strand, within GFF1105 at 291.806 kb on - strand, within GFF1105 at 291.825 kb on - strand, within GFF1105 at 291.825 kb on - strand, within GFF1105 at 291.944 kb on - strand at 291.944 kb on - strand at 292.054 kb on + strand at 292.058 kb on - strand at 292.061 kb on + strand at 292.062 kb on - strand at 292.064 kb on + strand at 292.065 kb on - strand at 292.074 kb on + strand at 292.229 kb on + strand, within GFF1106 at 292.310 kb on + strand, within GFF1106 at 292.310 kb on + strand, within GFF1106 at 292.311 kb on - strand, within GFF1106 at 292.311 kb on - strand, within GFF1106 at 292.332 kb on - strand, within GFF1106 at 292.567 kb on + strand, within GFF1106 at 292.567 kb on + strand, within GFF1106 at 292.568 kb on - strand, within GFF1106 at 292.656 kb on - strand, within GFF1106 at 292.728 kb on + strand, within GFF1106 at 292.728 kb on + strand, within GFF1106 at 292.747 kb on + strand, within GFF1106 at 292.827 kb on + strand at 292.827 kb on + strand at 292.828 kb on - strand at 292.828 kb on - strand at 292.828 kb on - strand at 292.950 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 291,060 - GFF1104 0.54 +0.0 291,071 - GFF1104 0.56 +1.0 291,071 - GFF1104 0.56 +0.6 291,134 + GFF1104 0.69 -0.3 291,294 + +1.1 291,317 + -1.3 291,320 - -0.3 291,320 - +0.1 291,381 + +0.1 291,386 - +0.1 291,505 - GFF1105 0.27 +0.2 291,577 + GFF1105 0.37 -0.1 291,577 + GFF1105 0.37 +1.1 291,743 - GFF1105 0.62 +2.5 291,805 + GFF1105 0.72 -0.2 291,805 + GFF1105 0.72 +1.3 291,806 - GFF1105 0.72 -0.9 291,825 - GFF1105 0.75 +1.0 291,825 - GFF1105 0.75 -0.5 291,944 - -2.3 291,944 - -0.1 292,054 + +0.5 292,058 - +1.5 292,061 + +0.4 292,062 - -0.5 292,064 + +0.7 292,065 - +1.4 292,074 + -0.1 292,229 + GFF1106 0.20 +0.5 292,310 + GFF1106 0.30 +4.2 292,310 + GFF1106 0.30 +0.4 292,311 - GFF1106 0.30 +1.8 292,311 - GFF1106 0.30 -0.7 292,332 - GFF1106 0.32 +1.0 292,567 + GFF1106 0.60 -0.5 292,567 + GFF1106 0.60 +0.3 292,568 - GFF1106 0.60 -1.1 292,656 - GFF1106 0.71 +0.2 292,728 + GFF1106 0.79 +3.5 292,728 + GFF1106 0.79 +0.3 292,747 + GFF1106 0.81 +0.7 292,827 + +0.2 292,827 + -1.7 292,828 - +0.3 292,828 - -0.1 292,828 - +2.3 292,950 + -0.7
Or see this region's nucleotide sequence