Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1084 and GFF1085 are separated by 56 nucleotides GFF1085 and GFF1086 are separated by 149 nucleotides GFF1086 and GFF1087 are separated by 35 nucleotides GFF1087 and GFF1088 are separated by 20 nucleotides GFF1088 and GFF1089 are separated by 168 nucleotides
GFF1084 - Molybdopterin biosynthesis molybdochelatase MogA, at 269,513 to 270,103
GFF1084
GFF1085 - YaaH protein, at 270,160 to 270,726
GFF1085
GFF1086 - FIG00553873: hypothetical protein, at 270,876 to 271,589
GFF1086
GFF1087 - FIG00638537: hypothetical protein, at 271,625 to 272,020
GFF1087
GFF1088 - FIG01045785: hypothetical protein, at 272,041 to 272,208
GFF1088
GFF1089 - Chaperone protein DnaK, at 272,377 to 274,293
GFF1089
Position (kb) 270
271
272 Strain fitness (log2 ratio) -2
-1
0
1
2 at 269.950 kb on - strand, within GFF1084 at 270.009 kb on + strand, within GFF1084 at 270.010 kb on - strand, within GFF1084 at 270.030 kb on - strand, within GFF1084 at 270.119 kb on - strand at 270.150 kb on + strand at 270.231 kb on - strand, within GFF1085 at 270.231 kb on - strand, within GFF1085 at 270.280 kb on - strand, within GFF1085 at 270.280 kb on - strand, within GFF1085 at 270.301 kb on - strand, within GFF1085 at 270.390 kb on + strand, within GFF1085 at 270.391 kb on - strand, within GFF1085 at 270.422 kb on + strand, within GFF1085 at 270.433 kb on + strand, within GFF1085 at 270.520 kb on - strand, within GFF1085 at 270.541 kb on - strand, within GFF1085 at 270.705 kb on + strand at 270.714 kb on + strand at 270.715 kb on - strand at 270.746 kb on - strand at 270.802 kb on + strand at 270.806 kb on - strand at 270.829 kb on - strand at 270.829 kb on - strand at 270.829 kb on - strand at 270.835 kb on + strand at 270.836 kb on - strand at 271.461 kb on - strand, within GFF1086 at 271.461 kb on - strand, within GFF1086 at 271.497 kb on - strand, within GFF1086 at 271.539 kb on - strand at 271.557 kb on + strand at 271.558 kb on - strand at 271.573 kb on - strand at 271.573 kb on - strand at 271.576 kb on + strand at 271.576 kb on + strand at 271.646 kb on - strand at 271.727 kb on - strand, within GFF1087 at 271.979 kb on + strand, within GFF1087 at 271.980 kb on - strand, within GFF1087 at 272.062 kb on - strand, within GFF1088 at 272.258 kb on + strand at 272.258 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 269,950 - GFF1084 0.74 -0.4 270,009 + GFF1084 0.84 +0.2 270,010 - GFF1084 0.84 -1.0 270,030 - GFF1084 0.87 +0.9 270,119 - -0.2 270,150 + -0.4 270,231 - GFF1085 0.13 +0.2 270,231 - GFF1085 0.13 -0.4 270,280 - GFF1085 0.21 +0.4 270,280 - GFF1085 0.21 -0.2 270,301 - GFF1085 0.25 +1.0 270,390 + GFF1085 0.41 -0.4 270,391 - GFF1085 0.41 -1.7 270,422 + GFF1085 0.46 -1.7 270,433 + GFF1085 0.48 -0.7 270,520 - GFF1085 0.63 -2.2 270,541 - GFF1085 0.67 -1.0 270,705 + -0.2 270,714 + -0.4 270,715 - -1.1 270,746 - +0.3 270,802 + -0.2 270,806 - +2.3 270,829 - -0.8 270,829 - -0.2 270,829 - -1.4 270,835 + +0.4 270,836 - +0.5 271,461 - GFF1086 0.82 +0.1 271,461 - GFF1086 0.82 -0.4 271,497 - GFF1086 0.87 -0.2 271,539 - +0.0 271,557 + -0.7 271,558 - -0.0 271,573 - +0.9 271,573 - +0.0 271,576 + -0.1 271,576 + +1.0 271,646 - +0.0 271,727 - GFF1087 0.26 +0.8 271,979 + GFF1087 0.89 -0.7 271,980 - GFF1087 0.90 -0.1 272,062 - GFF1088 0.12 +1.7 272,258 + -0.6 272,258 + -0.2
Or see this region's nucleotide sequence