Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1080 and GFF1081 are separated by 93 nucleotides GFF1081 and GFF1082 are separated by 78 nucleotides
GFF1080 - Threonine synthase (EC 4.2.3.1), at 264,518 to 265,804
GFF1080
GFF1081 - UPF0246 protein YaaA, at 265,898 to 266,671
GFF1081
GFF1082 - Putative alanine/glycine transport protein, at 266,750 to 268,207
GFF1082
Position (kb) 265
266
267 Strain fitness (log2 ratio) -2
-1
0
1
2 at 264.987 kb on - strand, within GFF1080 at 265.162 kb on + strand, within GFF1080 at 265.191 kb on - strand, within GFF1080 at 265.193 kb on + strand, within GFF1080 at 265.517 kb on - strand, within GFF1080 at 265.519 kb on + strand, within GFF1080 at 265.520 kb on - strand, within GFF1080 at 265.561 kb on + strand, within GFF1080 at 265.625 kb on + strand, within GFF1080 at 266.061 kb on - strand, within GFF1081 at 266.303 kb on + strand, within GFF1081 at 266.304 kb on - strand, within GFF1081 at 266.304 kb on - strand, within GFF1081 at 266.329 kb on - strand, within GFF1081 at 266.329 kb on - strand, within GFF1081 at 266.345 kb on - strand, within GFF1081 at 266.399 kb on + strand, within GFF1081 at 266.409 kb on + strand, within GFF1081 at 266.671 kb on + strand at 266.672 kb on - strand at 266.834 kb on + strand at 266.834 kb on + strand at 266.835 kb on - strand at 266.860 kb on - strand at 266.877 kb on + strand at 266.877 kb on + strand at 266.877 kb on + strand at 266.878 kb on - strand at 266.878 kb on - strand at 266.878 kb on - strand at 266.878 kb on - strand at 266.926 kb on + strand, within GFF1082 at 266.958 kb on - strand, within GFF1082 at 267.073 kb on - strand, within GFF1082 at 267.077 kb on - strand, within GFF1082 at 267.092 kb on - strand, within GFF1082 at 267.273 kb on - strand, within GFF1082 at 267.400 kb on + strand, within GFF1082 at 267.401 kb on - strand, within GFF1082 at 267.522 kb on - strand, within GFF1082 at 267.543 kb on - strand, within GFF1082 at 267.608 kb on + strand, within GFF1082
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 264,987 - GFF1080 0.36 -0.3 265,162 + GFF1080 0.50 +0.3 265,191 - GFF1080 0.52 +0.6 265,193 + GFF1080 0.52 -0.7 265,517 - GFF1080 0.78 +2.5 265,519 + GFF1080 0.78 -0.8 265,520 - GFF1080 0.78 -1.6 265,561 + GFF1080 0.81 -1.7 265,625 + GFF1080 0.86 -0.7 266,061 - GFF1081 0.21 -0.9 266,303 + GFF1081 0.52 +0.1 266,304 - GFF1081 0.52 +0.7 266,304 - GFF1081 0.52 +0.6 266,329 - GFF1081 0.56 -0.6 266,329 - GFF1081 0.56 -0.2 266,345 - GFF1081 0.58 +0.3 266,399 + GFF1081 0.65 -0.8 266,409 + GFF1081 0.66 +0.6 266,671 + +0.6 266,672 - +0.6 266,834 + +0.5 266,834 + -1.4 266,835 - -2.4 266,860 - -1.0 266,877 + -2.0 266,877 + -0.4 266,877 + -1.0 266,878 - -1.3 266,878 - -1.4 266,878 - -1.2 266,878 - -0.6 266,926 + GFF1082 0.12 +0.9 266,958 - GFF1082 0.14 -0.1 267,073 - GFF1082 0.22 -1.6 267,077 - GFF1082 0.22 -0.6 267,092 - GFF1082 0.23 +0.8 267,273 - GFF1082 0.36 -1.3 267,400 + GFF1082 0.45 -1.8 267,401 - GFF1082 0.45 -0.5 267,522 - GFF1082 0.53 -1.9 267,543 - GFF1082 0.54 -1.0 267,608 + GFF1082 0.59 -0.6
Or see this region's nucleotide sequence