Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1022 and GFF1023 overlap by 10 nucleotides GFF1023 and GFF1024 are separated by 13 nucleotides GFF1024 and GFF1025 are separated by 375 nucleotides
GFF1022 - FIG001826: putative inner membrane protein, at 207,516 to 207,989
GFF1022
GFF1023 - FIG023911: putative membrane protein, at 207,980 to 208,924
GFF1023
GFF1024 - FIG01045504: hypothetical protein, at 208,938 to 209,093
GFF1024
GFF1025 - Putative regulator, at 209,469 to 210,194
GFF1025
Position (kb) 208
209
210 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 207.969 kb on + strand at 207.981 kb on + strand at 207.981 kb on + strand at 207.983 kb on + strand at 207.984 kb on - strand at 208.004 kb on + strand at 208.084 kb on + strand, within GFF1023 at 208.084 kb on + strand, within GFF1023 at 208.085 kb on - strand, within GFF1023 at 208.087 kb on + strand, within GFF1023 at 208.087 kb on + strand, within GFF1023 at 208.087 kb on + strand, within GFF1023 at 208.087 kb on + strand, within GFF1023 at 208.088 kb on - strand, within GFF1023 at 208.088 kb on - strand, within GFF1023 at 208.103 kb on - strand, within GFF1023 at 208.117 kb on + strand, within GFF1023 at 208.148 kb on + strand, within GFF1023 at 208.149 kb on - strand, within GFF1023 at 208.293 kb on + strand, within GFF1023 at 208.293 kb on + strand, within GFF1023 at 208.294 kb on - strand, within GFF1023 at 208.294 kb on - strand, within GFF1023 at 208.294 kb on - strand, within GFF1023 at 208.301 kb on + strand, within GFF1023 at 208.489 kb on + strand, within GFF1023 at 208.489 kb on + strand, within GFF1023 at 208.516 kb on + strand, within GFF1023 at 208.516 kb on + strand, within GFF1023 at 208.516 kb on + strand, within GFF1023 at 208.517 kb on - strand, within GFF1023 at 208.716 kb on - strand, within GFF1023 at 208.716 kb on - strand, within GFF1023 at 208.716 kb on - strand, within GFF1023 at 208.848 kb on - strand at 208.940 kb on - strand at 208.974 kb on + strand, within GFF1024 at 209.288 kb on + strand at 209.519 kb on + strand at 209.544 kb on - strand, within GFF1025 at 209.609 kb on - strand, within GFF1025 at 209.716 kb on + strand, within GFF1025 at 210.084 kb on + strand, within GFF1025 at 210.085 kb on - strand, within GFF1025
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI remove 207,969 + -0.6 207,981 + -0.5 207,981 + +0.3 207,983 + -0.5 207,984 - -0.8 208,004 + -0.7 208,084 + GFF1023 0.11 -0.6 208,084 + GFF1023 0.11 -0.3 208,085 - GFF1023 0.11 +0.9 208,087 + GFF1023 0.11 +0.2 208,087 + GFF1023 0.11 +0.5 208,087 + GFF1023 0.11 -0.9 208,087 + GFF1023 0.11 +0.3 208,088 - GFF1023 0.11 +1.0 208,088 - GFF1023 0.11 -0.4 208,103 - GFF1023 0.13 -0.2 208,117 + GFF1023 0.14 +0.3 208,148 + GFF1023 0.18 +0.1 208,149 - GFF1023 0.18 +1.0 208,293 + GFF1023 0.33 -0.3 208,293 + GFF1023 0.33 -0.8 208,294 - GFF1023 0.33 -0.9 208,294 - GFF1023 0.33 +0.6 208,294 - GFF1023 0.33 -0.6 208,301 + GFF1023 0.34 +0.2 208,489 + GFF1023 0.54 -2.5 208,489 + GFF1023 0.54 -0.3 208,516 + GFF1023 0.57 -2.3 208,516 + GFF1023 0.57 +0.2 208,516 + GFF1023 0.57 +0.0 208,517 - GFF1023 0.57 +0.6 208,716 - GFF1023 0.78 +2.8 208,716 - GFF1023 0.78 +1.0 208,716 - GFF1023 0.78 -0.3 208,848 - +0.1 208,940 - -0.1 208,974 + GFF1024 0.23 +1.0 209,288 + -0.5 209,519 + +3.3 209,544 - GFF1025 0.10 +2.3 209,609 - GFF1025 0.19 +0.2 209,716 + GFF1025 0.34 +1.6 210,084 + GFF1025 0.85 +1.8 210,085 - GFF1025 0.85 -0.1
Or see this region's nucleotide sequence