Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1002

Experiment: LB_plus_SM_buffer with ES18h1_phage 0.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1001 and GFF1002 are separated by 157 nucleotidesGFF1002 and GFF1003 are separated by 197 nucleotides GFF1001 - Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72), at 182,364 to 183,953 GFF1001 GFF1002 - Type I restriction-modification system, restriction subunit R (EC 3.1.21.3), at 184,111 to 187,620 GFF1002 GFF1003 - Mrr restriction system protein, at 187,818 to 188,732 GFF1003 Position (kb) 184 185 186 187 188Strain fitness (log2 ratio) -2 -1 0 1 2 3at 183.170 kb on + strand, within GFF1001at 183.170 kb on + strand, within GFF1001at 183.508 kb on + strand, within GFF1001at 183.562 kb on + strand, within GFF1001at 183.562 kb on + strand, within GFF1001at 183.562 kb on + strand, within GFF1001at 183.562 kb on + strand, within GFF1001at 183.563 kb on - strand, within GFF1001at 183.608 kb on - strand, within GFF1001at 183.721 kb on - strand, within GFF1001at 184.054 kb on - strandat 184.054 kb on - strandat 184.104 kb on + strandat 184.113 kb on - strandat 184.133 kb on + strandat 184.133 kb on + strandat 184.648 kb on + strand, within GFF1002at 184.864 kb on + strand, within GFF1002at 184.890 kb on - strand, within GFF1002at 184.890 kb on - strand, within GFF1002at 184.890 kb on - strand, within GFF1002at 184.983 kb on - strand, within GFF1002at 185.082 kb on - strand, within GFF1002at 185.086 kb on - strand, within GFF1002at 185.107 kb on - strand, within GFF1002at 185.202 kb on + strand, within GFF1002at 185.211 kb on + strand, within GFF1002at 185.255 kb on - strand, within GFF1002at 185.627 kb on + strand, within GFF1002at 185.718 kb on - strand, within GFF1002at 185.913 kb on + strand, within GFF1002at 185.913 kb on + strand, within GFF1002at 185.914 kb on - strand, within GFF1002at 186.172 kb on + strand, within GFF1002at 186.284 kb on + strand, within GFF1002at 186.285 kb on - strand, within GFF1002at 186.285 kb on - strand, within GFF1002at 186.441 kb on + strand, within GFF1002at 186.549 kb on - strand, within GFF1002at 186.724 kb on + strand, within GFF1002at 186.724 kb on + strand, within GFF1002at 186.728 kb on + strand, within GFF1002at 186.762 kb on + strand, within GFF1002at 186.763 kb on - strand, within GFF1002at 186.830 kb on - strand, within GFF1002at 186.950 kb on + strand, within GFF1002at 186.951 kb on - strand, within GFF1002at 187.527 kb on + strandat 187.528 kb on - strandat 187.571 kb on + strandat 187.789 kb on + strandat 187.790 kb on - strandat 187.795 kb on - strandat 187.834 kb on - strandat 188.186 kb on - strand, within GFF1003at 188.344 kb on + strand, within GFF1003at 188.344 kb on + strand, within GFF1003at 188.345 kb on - strand, within GFF1003at 188.432 kb on + strand, within GFF1003at 188.432 kb on + strand, within GFF1003at 188.524 kb on - strand, within GFF1003at 188.524 kb on - strand, within GFF1003at 188.602 kb on - strand, within GFF1003at 188.602 kb on - strand, within GFF1003at 188.607 kb on - strand, within GFF1003

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.75 MOI
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183,170 + GFF1001 0.51 -0.6
183,170 + GFF1001 0.51 -1.1
183,508 + GFF1001 0.72 +0.8
183,562 + GFF1001 0.75 +0.3
183,562 + GFF1001 0.75 +0.1
183,562 + GFF1001 0.75 +1.0
183,562 + GFF1001 0.75 -0.4
183,563 - GFF1001 0.75 -1.0
183,608 - GFF1001 0.78 -1.5
183,721 - GFF1001 0.85 -0.3
184,054 - -0.5
184,054 - -0.9
184,104 + -0.2
184,113 - -2.7
184,133 + +0.2
184,133 + +1.8
184,648 + GFF1002 0.15 -1.2
184,864 + GFF1002 0.21 +2.0
184,890 - GFF1002 0.22 -0.7
184,890 - GFF1002 0.22 -0.3
184,890 - GFF1002 0.22 -0.3
184,983 - GFF1002 0.25 +0.3
185,082 - GFF1002 0.28 -0.0
185,086 - GFF1002 0.28 -0.0
185,107 - GFF1002 0.28 +3.0
185,202 + GFF1002 0.31 -0.6
185,211 + GFF1002 0.31 +1.2
185,255 - GFF1002 0.33 +0.0
185,627 + GFF1002 0.43 +0.3
185,718 - GFF1002 0.46 +0.7
185,913 + GFF1002 0.51 -0.3
185,913 + GFF1002 0.51 +1.0
185,914 - GFF1002 0.51 -0.2
186,172 + GFF1002 0.59 +1.0
186,284 + GFF1002 0.62 -0.8
186,285 - GFF1002 0.62 -0.2
186,285 - GFF1002 0.62 -1.3
186,441 + GFF1002 0.66 +0.0
186,549 - GFF1002 0.69 +0.9
186,724 + GFF1002 0.74 -1.0
186,724 + GFF1002 0.74 -0.2
186,728 + GFF1002 0.75 +1.5
186,762 + GFF1002 0.76 +0.5
186,763 - GFF1002 0.76 -0.9
186,830 - GFF1002 0.77 +0.7
186,950 + GFF1002 0.81 +0.0
186,951 - GFF1002 0.81 +0.6
187,527 + -0.7
187,528 - +0.1
187,571 + -0.2
187,789 + -0.9
187,790 - +1.8
187,795 - -0.3
187,834 - +0.4
188,186 - GFF1003 0.40 -1.0
188,344 + GFF1003 0.57 +0.9
188,344 + GFF1003 0.57 +2.8
188,345 - GFF1003 0.58 -1.8
188,432 + GFF1003 0.67 +0.9
188,432 + GFF1003 0.67 +0.0
188,524 - GFF1003 0.77 -1.5
188,524 - GFF1003 0.77 -0.6
188,602 - GFF1003 0.86 +1.2
188,602 - GFF1003 0.86 +0.8
188,607 - GFF1003 0.86 +1.3

Or see this region's nucleotide sequence