Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF912 and GFF913 overlap by 17 nucleotides GFF913 and GFF914 overlap by 4 nucleotides GFF914 and GFF915 are separated by 16 nucleotides
GFF912 - Metallo-dependent hydrolases, subgroup B, at 89,820 to 90,953
GFF912
GFF913 - D-Glucosaminate-6-phosphate ammonia-lyase (EC 4.3.1.-), at 90,937 to 92,055
GFF913
GFF914 - 2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14) in D-glucosaminate utilization operon, at 92,052 to 92,792
GFF914
GFF915 - Putative BglB-family transcriptional antiterminator, at 92,809 to 94,722
GFF915
Position (kb)
90
91
92
93 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 90.096 kb on - strand, within GFF912 at 90.142 kb on - strand, within GFF912 at 90.173 kb on + strand, within GFF912 at 90.174 kb on - strand, within GFF912 at 90.254 kb on + strand, within GFF912 at 90.367 kb on + strand, within GFF912 at 90.367 kb on + strand, within GFF912 at 90.368 kb on - strand, within GFF912 at 90.368 kb on - strand, within GFF912 at 90.371 kb on + strand, within GFF912 at 90.371 kb on + strand, within GFF912 at 90.372 kb on - strand, within GFF912 at 90.372 kb on - strand, within GFF912 at 90.372 kb on - strand, within GFF912 at 90.585 kb on - strand, within GFF912 at 90.605 kb on + strand, within GFF912 at 90.605 kb on + strand, within GFF912 at 90.606 kb on - strand, within GFF912 at 90.625 kb on - strand, within GFF912 at 90.625 kb on - strand, within GFF912 at 90.665 kb on + strand, within GFF912 at 90.806 kb on + strand, within GFF912 at 90.807 kb on - strand, within GFF912 at 90.813 kb on - strand, within GFF912 at 90.856 kb on + strand at 90.856 kb on + strand at 90.857 kb on - strand at 90.909 kb on + strand at 91.103 kb on - strand, within GFF913 at 91.227 kb on + strand, within GFF913 at 91.228 kb on - strand, within GFF913 at 91.381 kb on - strand, within GFF913 at 91.405 kb on + strand, within GFF913 at 91.405 kb on + strand, within GFF913 at 91.405 kb on + strand, within GFF913 at 91.408 kb on - strand, within GFF913 at 91.427 kb on + strand, within GFF913 at 91.480 kb on - strand, within GFF913 at 91.554 kb on + strand, within GFF913 at 91.558 kb on + strand, within GFF913 at 91.632 kb on - strand, within GFF913 at 91.752 kb on - strand, within GFF913 at 91.811 kb on + strand, within GFF913 at 91.921 kb on + strand, within GFF913 at 91.930 kb on + strand, within GFF913 at 91.931 kb on - strand, within GFF913 at 92.001 kb on - strand at 92.220 kb on + strand, within GFF914 at 92.232 kb on - strand, within GFF914 at 92.311 kb on + strand, within GFF914 at 92.482 kb on + strand, within GFF914 at 92.568 kb on + strand, within GFF914 at 92.569 kb on - strand, within GFF914 at 92.570 kb on + strand, within GFF914 at 92.571 kb on - strand, within GFF914 at 92.578 kb on + strand, within GFF914 at 92.681 kb on - strand, within GFF914 at 92.703 kb on + strand, within GFF914 at 92.706 kb on - strand, within GFF914 at 92.706 kb on - strand, within GFF914 at 92.720 kb on + strand at 92.720 kb on + strand at 92.761 kb on + strand at 92.761 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 90,096 - GFF912 0.24 +0.4 90,142 - GFF912 0.28 -0.3 90,173 + GFF912 0.31 +0.2 90,174 - GFF912 0.31 +2.7 90,254 + GFF912 0.38 -0.2 90,367 + GFF912 0.48 -0.4 90,367 + GFF912 0.48 +0.3 90,368 - GFF912 0.48 -0.2 90,368 - GFF912 0.48 -2.3 90,371 + GFF912 0.49 +0.3 90,371 + GFF912 0.49 +1.0 90,372 - GFF912 0.49 +0.0 90,372 - GFF912 0.49 +1.3 90,372 - GFF912 0.49 -0.4 90,585 - GFF912 0.67 +0.2 90,605 + GFF912 0.69 -0.3 90,605 + GFF912 0.69 -0.5 90,606 - GFF912 0.69 -0.8 90,625 - GFF912 0.71 -0.1 90,625 - GFF912 0.71 -0.2 90,665 + GFF912 0.75 -0.6 90,806 + GFF912 0.87 +4.1 90,807 - GFF912 0.87 +1.0 90,813 - GFF912 0.88 -0.8 90,856 + +0.4 90,856 + -1.0 90,857 - -0.7 90,909 + -1.6 91,103 - GFF913 0.15 +0.1 91,227 + GFF913 0.26 -0.3 91,228 - GFF913 0.26 -1.5 91,381 - GFF913 0.40 -0.3 91,405 + GFF913 0.42 -0.5 91,405 + GFF913 0.42 -0.1 91,405 + GFF913 0.42 -0.9 91,408 - GFF913 0.42 +0.9 91,427 + GFF913 0.44 +0.3 91,480 - GFF913 0.49 +1.1 91,554 + GFF913 0.55 +1.2 91,558 + GFF913 0.55 -0.2 91,632 - GFF913 0.62 +0.2 91,752 - GFF913 0.73 +0.3 91,811 + GFF913 0.78 +0.1 91,921 + GFF913 0.88 +0.4 91,930 + GFF913 0.89 +0.5 91,931 - GFF913 0.89 +5.3 92,001 - +1.6 92,220 + GFF914 0.23 +0.3 92,232 - GFF914 0.24 -0.5 92,311 + GFF914 0.35 +0.4 92,482 + GFF914 0.58 -0.1 92,568 + GFF914 0.70 +0.6 92,569 - GFF914 0.70 +0.3 92,570 + GFF914 0.70 +0.6 92,571 - GFF914 0.70 +0.5 92,578 + GFF914 0.71 +0.5 92,681 - GFF914 0.85 -1.5 92,703 + GFF914 0.88 +0.4 92,706 - GFF914 0.88 +0.6 92,706 - GFF914 0.88 +0.5 92,720 + +6.4 92,720 + -0.1 92,761 + +0.0 92,761 + +0.3
Or see this region's nucleotide sequence