Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF864 and GFF865 are separated by 87 nucleotides GFF865 and GFF866 are separated by 57 nucleotides GFF866 and GFF867 are separated by 12 nucleotides GFF867 and GFF868 are separated by 33 nucleotides
GFF864 - NADPH:quinone oxidoreductase 2, at 41,529 to 42,377
GFF864
GFF865 - Redox-sensing transcriptional regulator QorR, at 42,465 to 42,851
GFF865
GFF866 - '2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16)' transl_table=11, at 42,909 to 44,852
GFF866
GFF867 - FIG01048799: hypothetical protein, at 44,865 to 45,086
GFF867
GFF868 - '3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7)' transl_table=11, at 45,120 to 45,860
GFF868
GFF869 - Protein ytfJ precursor, at 45,850 to 46,407
GFF869
Position (kb) 42
43
44
45 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7 at 41.973 kb on + strand, within GFF864 at 41.974 kb on - strand, within GFF864 at 41.991 kb on - strand, within GFF864 at 42.024 kb on - strand, within GFF864 at 42.063 kb on + strand, within GFF864 at 42.064 kb on - strand, within GFF864 at 42.075 kb on - strand, within GFF864 at 42.077 kb on - strand, within GFF864 at 42.077 kb on - strand, within GFF864 at 42.162 kb on + strand, within GFF864 at 42.164 kb on + strand, within GFF864 at 42.165 kb on - strand, within GFF864 at 42.165 kb on - strand, within GFF864 at 42.165 kb on - strand, within GFF864 at 42.165 kb on - strand, within GFF864 at 42.165 kb on - strand, within GFF864 at 42.177 kb on - strand, within GFF864 at 42.177 kb on - strand, within GFF864 at 42.319 kb on - strand at 42.368 kb on + strand at 42.411 kb on + strand at 42.476 kb on + strand at 42.477 kb on - strand at 42.664 kb on + strand, within GFF865 at 42.693 kb on - strand, within GFF865 at 42.768 kb on - strand, within GFF865 at 42.814 kb on + strand at 42.910 kb on + strand at 42.911 kb on - strand at 42.932 kb on - strand at 43.039 kb on + strand at 43.079 kb on + strand at 43.080 kb on - strand at 43.080 kb on - strand at 43.269 kb on - strand, within GFF866 at 43.293 kb on - strand, within GFF866 at 43.334 kb on - strand, within GFF866 at 43.445 kb on + strand, within GFF866 at 43.446 kb on - strand, within GFF866 at 43.458 kb on - strand, within GFF866 at 43.491 kb on + strand, within GFF866 at 43.610 kb on + strand, within GFF866 at 43.626 kb on - strand, within GFF866 at 43.696 kb on + strand, within GFF866 at 43.697 kb on - strand, within GFF866 at 43.725 kb on + strand, within GFF866 at 43.726 kb on - strand, within GFF866 at 43.757 kb on - strand, within GFF866 at 43.762 kb on + strand, within GFF866 at 43.763 kb on - strand, within GFF866 at 43.847 kb on + strand, within GFF866 at 43.848 kb on - strand, within GFF866 at 43.889 kb on - strand, within GFF866 at 43.889 kb on - strand, within GFF866 at 43.893 kb on - strand, within GFF866 at 44.093 kb on + strand, within GFF866 at 44.155 kb on + strand, within GFF866 at 44.190 kb on + strand, within GFF866 at 44.191 kb on - strand, within GFF866 at 44.389 kb on + strand, within GFF866 at 44.495 kb on + strand, within GFF866 at 44.594 kb on + strand, within GFF866 at 44.594 kb on + strand, within GFF866 at 44.594 kb on + strand, within GFF866 at 44.597 kb on - strand, within GFF866 at 44.695 kb on - strand at 44.695 kb on - strand at 44.729 kb on - strand at 44.846 kb on + strand at 44.846 kb on + strand at 44.867 kb on - strand at 44.878 kb on - strand at 44.901 kb on - strand, within GFF867 at 45.023 kb on + strand, within GFF867 at 45.073 kb on + strand at 45.092 kb on + strand at 45.133 kb on + strand at 45.134 kb on - strand at 45.177 kb on + strand at 45.177 kb on + strand at 45.489 kb on + strand, within GFF868 at 45.490 kb on - strand, within GFF868 at 45.490 kb on - strand, within GFF868 at 45.507 kb on - strand, within GFF868 at 45.537 kb on - strand, within GFF868 at 45.808 kb on - strand at 45.851 kb on + strand at 45.852 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 41,973 + GFF864 0.52 +1.2 41,974 - GFF864 0.52 +0.8 41,991 - GFF864 0.54 -0.1 42,024 - GFF864 0.58 -0.0 42,063 + GFF864 0.63 +0.0 42,064 - GFF864 0.63 -1.6 42,075 - GFF864 0.64 -0.2 42,077 - GFF864 0.65 +0.3 42,077 - GFF864 0.65 -0.6 42,162 + GFF864 0.75 -3.2 42,164 + GFF864 0.75 +0.0 42,165 - GFF864 0.75 +0.9 42,165 - GFF864 0.75 +0.8 42,165 - GFF864 0.75 -0.5 42,165 - GFF864 0.75 -1.9 42,165 - GFF864 0.75 +1.7 42,177 - GFF864 0.76 +0.6 42,177 - GFF864 0.76 -0.6 42,319 - +0.7 42,368 + -0.6 42,411 + +7.0 42,476 + -1.2 42,477 - +0.3 42,664 + GFF865 0.51 +0.2 42,693 - GFF865 0.59 -2.1 42,768 - GFF865 0.78 -0.0 42,814 + -0.8 42,910 + +2.2 42,911 - -1.6 42,932 - +0.2 43,039 + +0.4 43,079 + -1.2 43,080 - +0.3 43,080 - -0.2 43,269 - GFF866 0.19 +0.8 43,293 - GFF866 0.20 +3.7 43,334 - GFF866 0.22 +0.3 43,445 + GFF866 0.28 +0.6 43,446 - GFF866 0.28 +0.3 43,458 - GFF866 0.28 +0.7 43,491 + GFF866 0.30 +0.1 43,610 + GFF866 0.36 +0.0 43,626 - GFF866 0.37 -0.7 43,696 + GFF866 0.40 +7.6 43,697 - GFF866 0.41 -0.2 43,725 + GFF866 0.42 -0.0 43,726 - GFF866 0.42 +1.3 43,757 - GFF866 0.44 -0.6 43,762 + GFF866 0.44 +0.1 43,763 - GFF866 0.44 +1.8 43,847 + GFF866 0.48 +0.5 43,848 - GFF866 0.48 -1.2 43,889 - GFF866 0.50 +0.8 43,889 - GFF866 0.50 -0.3 43,893 - GFF866 0.51 +0.8 44,093 + GFF866 0.61 +0.4 44,155 + GFF866 0.64 -0.8 44,190 + GFF866 0.66 +0.0 44,191 - GFF866 0.66 -1.5 44,389 + GFF866 0.76 +0.0 44,495 + GFF866 0.82 +0.1 44,594 + GFF866 0.87 +1.4 44,594 + GFF866 0.87 -0.3 44,594 + GFF866 0.87 +5.2 44,597 - GFF866 0.87 +1.3 44,695 - +1.1 44,695 - -0.9 44,729 - +0.4 44,846 + +1.0 44,846 + -0.5 44,867 - +0.1 44,878 - +0.5 44,901 - GFF867 0.16 -1.6 45,023 + GFF867 0.71 -0.4 45,073 + +0.1 45,092 + -0.1 45,133 + -0.4 45,134 - +0.2 45,177 + +0.3 45,177 + -0.0 45,489 + GFF868 0.50 -1.0 45,490 - GFF868 0.50 +0.2 45,490 - GFF868 0.50 +1.0 45,507 - GFF868 0.52 +0.1 45,537 - GFF868 0.56 +0.3 45,808 - +0.5 45,851 + +0.4 45,852 - -0.3
Or see this region's nucleotide sequence