Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF596 and GFF597 are separated by 108 nucleotides GFF597 and GFF598 are separated by 2 nucleotides GFF598 and GFF599 are separated by 87 nucleotides GFF599 and GFF600 are separated by 593 nucleotides
GFF596 - 'RNA 3'-terminal phosphate cyclase (EC 6.5.1.4)' transl_table=11, at 331,720 to 332,739
GFF596
GFF597 - DNA-damage-inducible protein J, at 332,848 to 333,108
GFF597
GFF598 - YafQ toxin protein, at 333,111 to 333,386
GFF598
GFF599 - Transcriptional activator of maltose regulon, MalT, at 333,474 to 336,179
GFF599
GFF600 - Maltodextrin phosphorylase (EC 2.4.1.1), at 336,773 to 339,166
GFF600
Position (kb) 333
334
335
336
337 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 332.656 kb on - strand at 332.737 kb on + strand at 332.748 kb on + strand at 332.786 kb on - strand at 332.819 kb on + strand at 332.832 kb on + strand at 333.017 kb on + strand, within GFF597 at 333.018 kb on - strand, within GFF597 at 333.039 kb on + strand, within GFF597 at 333.179 kb on + strand, within GFF598 at 333.180 kb on - strand, within GFF598 at 333.180 kb on - strand, within GFF598 at 333.207 kb on - strand, within GFF598 at 333.251 kb on + strand, within GFF598 at 333.252 kb on - strand, within GFF598 at 333.320 kb on + strand, within GFF598 at 333.321 kb on - strand, within GFF598 at 333.326 kb on + strand, within GFF598 at 333.384 kb on + strand at 333.406 kb on - strand at 333.578 kb on + strand at 333.755 kb on + strand, within GFF599 at 333.915 kb on + strand, within GFF599 at 333.982 kb on - strand, within GFF599 at 334.267 kb on - strand, within GFF599 at 334.604 kb on - strand, within GFF599 at 334.680 kb on - strand, within GFF599 at 334.718 kb on + strand, within GFF599 at 334.719 kb on - strand, within GFF599 at 334.990 kb on - strand, within GFF599 at 334.990 kb on - strand, within GFF599 at 335.024 kb on + strand, within GFF599 at 335.046 kb on - strand, within GFF599 at 335.098 kb on - strand, within GFF599 at 335.098 kb on - strand, within GFF599 at 335.098 kb on - strand, within GFF599 at 335.098 kb on - strand, within GFF599 at 335.480 kb on + strand, within GFF599 at 335.792 kb on - strand, within GFF599 at 335.840 kb on + strand, within GFF599 at 335.841 kb on - strand, within GFF599 at 335.875 kb on + strand, within GFF599 at 335.876 kb on - strand, within GFF599 at 335.947 kb on - strand at 335.947 kb on - strand at 335.947 kb on - strand at 335.947 kb on - strand at 335.947 kb on - strand at 335.995 kb on + strand at 336.052 kb on + strand at 336.134 kb on + strand at 336.159 kb on + strand at 336.242 kb on - strand at 336.247 kb on + strand at 336.292 kb on + strand at 336.462 kb on - strand at 336.470 kb on + strand at 336.592 kb on + strand at 336.627 kb on + strand at 336.637 kb on + strand at 336.637 kb on + strand at 336.637 kb on + strand at 336.638 kb on - strand at 336.659 kb on + strand at 336.659 kb on + strand at 336.843 kb on + strand at 336.963 kb on - strand at 337.044 kb on + strand, within GFF600 at 337.063 kb on + strand, within GFF600
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 332,656 - +0.7 332,737 + +0.0 332,748 + -0.3 332,786 - +0.7 332,819 + +0.9 332,832 + -0.7 333,017 + GFF597 0.65 -1.6 333,018 - GFF597 0.65 -0.4 333,039 + GFF597 0.73 -0.3 333,179 + GFF598 0.25 +0.6 333,180 - GFF598 0.25 +1.2 333,180 - GFF598 0.25 +0.9 333,207 - GFF598 0.35 -0.6 333,251 + GFF598 0.51 -0.0 333,252 - GFF598 0.51 +0.3 333,320 + GFF598 0.76 -0.7 333,321 - GFF598 0.76 +1.5 333,326 + GFF598 0.78 +1.7 333,384 + +0.0 333,406 - -0.7 333,578 + +2.3 333,755 + GFF599 0.10 +1.0 333,915 + GFF599 0.16 +1.9 333,982 - GFF599 0.19 +0.1 334,267 - GFF599 0.29 -0.2 334,604 - GFF599 0.42 -1.8 334,680 - GFF599 0.45 -0.0 334,718 + GFF599 0.46 -0.3 334,719 - GFF599 0.46 -0.2 334,990 - GFF599 0.56 +0.7 334,990 - GFF599 0.56 -0.1 335,024 + GFF599 0.57 -0.6 335,046 - GFF599 0.58 -0.7 335,098 - GFF599 0.60 -0.0 335,098 - GFF599 0.60 +1.9 335,098 - GFF599 0.60 +1.4 335,098 - GFF599 0.60 -0.2 335,480 + GFF599 0.74 +0.1 335,792 - GFF599 0.86 -0.5 335,840 + GFF599 0.87 -0.3 335,841 - GFF599 0.87 +0.2 335,875 + GFF599 0.89 -0.2 335,876 - GFF599 0.89 -0.9 335,947 - -0.6 335,947 - +0.9 335,947 - +0.1 335,947 - -0.5 335,947 - +0.3 335,995 + -0.5 336,052 + -1.3 336,134 + -0.1 336,159 + +1.1 336,242 - +0.1 336,247 + +0.4 336,292 + -0.4 336,462 - -0.1 336,470 + -0.2 336,592 + -0.4 336,627 + -0.1 336,637 + +0.9 336,637 + +0.4 336,637 + +0.6 336,638 - +0.4 336,659 + -0.9 336,659 + -0.4 336,843 + -0.1 336,963 - +2.2 337,044 + GFF600 0.11 -0.3 337,063 + GFF600 0.12 +6.1
Or see this region's nucleotide sequence